2’3’-c-di-AM(PS)2 (Rp,Rp) (Synonyms: ADU-S100; MIW815; ML RR-S2 CDA)

Name: 2’3’-c-di-AM(PS)2 (Rp,Rp)
Brand: MedChemExpress
SKU: HY-12885
Rating: 5.0 (87 reviews)

Cat. No.: HY-12885 Purity: 99.59%: Data Sheet
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2’3’-c-di-AM(PS)2 (Rp,Rp) (ADU-S100), an activator of stimulator of interferon genes (STING), leads to potent and systemic tumor regression and immunity.

For research use only. We do not sell to patients.

CAS No. : 1638241-89-0

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
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Based on 87 publication(s) in Google Scholar

Other Forms of 2’3’-c-di-AM(PS)2 (Rp,Rp):

2’3’-c-di-AM(PS)2 (Rp,Rp) enantiomer ammonium salt In-stock
2’3’-c-di-AM(PS)2 (Rp,Rp) disodium salt In-stock
2’3’-c-di-AM(PS)2 (Rp,Rp) ammonium salt In-stock

87 Publications Citing Use of MCE 2’3’-c-di-AM(PS)2 (Rp,Rp)

Cell Proliferation/Viability Assay

In Vivo Efficacy Study

IHC

Flow Cytometry

RT-PCR

: 2’3’-c-di-AM(PS)2 (Rp,Rp) purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2025 Oct;26(10):1766-1780. [Abstract]; 2’3’-c-di-AM(PS)2 (Rp,Rp) (ADU-S100) (10 μM) was added to the culture of KPC cells or endothelial cells (HUVEC) for indicated time, and the activation of STING signaling was analyzed by IRF3 Ser385 phosphorylation. The quantification of phosphorylated IRF3 was normalized by total IRF3. β-actin was used as a loading control.

: 2’3’-c-di-AM(PS)2 (Rp,Rp) purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2025 Oct;26(10):1766-1780. [Abstract]; Leukocytes were isolated from peripheral blood or the peritoneum of normal mice and incubated in vitro with 7.2 μg/mL STING agonist 2’3’-c-di-AM(PS)2 (Rp,Rp) (ADU-S100) for 2 h at 37 °C. Phosphorylation of IRF3 in leukocyte subsets (CD11b⁺ myeloid cells, CD3⁺ cells, CD19⁺ cells, and NK1.1⁺ cells) was detected by α-phospho-IRF3 Ser385 antibody staining. Cells were permeabilized using eBioscience™ Transcription Factor Staining Buffer Set before staining. The percent fraction of phospho-IRF3⁺ cells in each subset of blood or peritoneal leukocytes was determined by flow cytometry.

: 2’3’-c-di-AM(PS)2 (Rp,Rp) purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2025 Oct;26(10):1766-1780. [Abstract]; STING agonist 2’3’-c-di-AM(PS)2 (Rp,Rp) (ADU-S100) (2 μg intratumoral) and/or anti-LTβR agonistic antibody (100 μg, intraperitoneal (i.p.)) were administered to mice bearing subcutaneous KPC tumors as monotherapy or in combination as indicated. Immunohistochemistry of B cells (CD19, brown) and HEVs (MECA-79, magenta) shows numerous TLS induced by combination therapy (day 14). Scale bars, 1 mm (low magnification) and 100 μm (high magnification).

: 2’3’-c-di-AM(PS)2 (Rp,Rp) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Apr;12(16):e2414110. [Abstract]; The relative mRNA expression of Ifnβ1 in 4T1 cells, MC38 cells and B16F10 cells after treatment with TACTIC, CS or ADU‐S100 (2’3’-c-di-AM(PS)2 (Rp,Rp) disodium salt, 20 µg/mL; 24 h) (n = 3).

: 2’3’-c-di-AM(PS)2 (Rp,Rp) purchased from MedChemExpress. Usage Cited in: Mol Ther. 2025 Sep 22:S1525-0016(25)00761-0. [Abstract]; Titration of the agonist ADU-S100 in B16-OVA tumor model. OVA mRNA, 10 μg; C1, 1.6 mg; ADU-S100 (2’3’-c-di-AM(PS)2 (Rp,Rp) disodium salt): 10/20/40 μg. n=5 mice per group. The results indicated that the ADU-S100 dosage used (20 μg/mice) was likely optimal for the C1-mRNA+ADU-S100(nano) cancer vaccine.

: 2’3’-c-di-AM(PS)2 (Rp,Rp) purchased from MedChemExpress. Usage Cited in: Mol Ther. 2025 Sep 22:S1525-0016(25)00761-0. [Abstract]; Representative images of tumor-infiltrating CD8⁺ T cells by immunohistochemistry (left) and quantification of CD8⁺ T cells (right). Scale bars: 100 μm (upper) and 50 μm (lower). Tumor-infiltrating immune cell analysis by immunohistochemistry demonstrated that CD8⁺ T cells were enriched in tumors from Sting^f/f Itgax-Cre^– mice immunized with C1-OVA mRNA+ADU-S100(nano), but not in tumors from Sting^f/f Itgax-Cre⁺ mice. The doses of mRNA vaccine per mouse in vivo were as follows: mRNA, 10 μg; C1, 1.6 mg; ADU-S100, 20 μg; c-di-AMP, 20 μg. C1-mRNA + ADU-S100(nano) was prepared by encapsulating ADU-S100 into the C1-mRNA nanovaccine during nanoparticle preparation.

: 2’3’-c-di-AM(PS)2 (Rp,Rp) purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2024 May 13;42(5):850-868.e9. [Abstract]; Cell viability assays of isogenic NTC, Trp53 KO, and Bak/Bax KO Eμ-Myc lymphoma cell lines treated with S63845 in combination with ADU-S100 (2’3’-c-di-AM(PS)2 (Rp,Rp) disodium salt, 2-10 μg/mL), MSA-2 (12-34 μM), or diABZI for 24 h.

: 2’3’-c-di-AM(PS)2 (Rp,Rp) purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2024 May 13;42(5):850-868.e9. [Abstract]; RT-qPCR for BH3-only and STING target genes in three independent isogenic NTC and Trp53 KO Em-Myc lymphoma cell lines treated with ADU-S100 (2’3’-c-di-AM(PS)2 (Rp,Rp) disodium salt, 10 μg/mL) for 24 h.Fold change is relative to the AH15A DMSO-treated NTC sample

: 2’3’-c-di-AM(PS)2 (Rp,Rp) purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2024 May 13;42(5):850-868.e9. [Abstract]; Cell viability assays of Eμ-Myc lymphoma cell lines treated with ADU-S100 (HY-12885A; AH15A/560: 0.5 μg/mL; 47A: 5 μg/mL) and/or TPCA-1 (HY-10074) for 24 h. Error bars represent SD for three independent experiments. Diagram illustrates how TPCA-1 acts to inhibit NF-κB signaling.

: 2’3’-c-di-AM(PS)2 (Rp,Rp) purchased from MedChemExpress. Usage Cited in: Nat Cell Biol. 2024 Sep;26(9):1585-1596. [Abstract]; Cell viability assays measuring 5-FU response in cGAS knockdown HCT116 cells stably expressing shRNA-resistant cGAS-S213D mutants or pretreated with 10 µM 2’3’-c-di-AM(PS)2 (Rp,Rp) (ADU-S100) for 6 hours.

: 2’3’-c-di-AM(PS)2 (Rp,Rp) purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Mar 13;14(1):1390. [Abstract]; ADU-S100 (10 μM; 48 h; labeled as STING) enhances type I interferon gene expression in human dendritic cells (DCs).

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Description

2’3’-c-di-AM(PS)2 (Rp,Rp) (ADU-S100), an activator of stimulator of interferon genes (STING), leads to potent and systemic tumor regression and immunity^[1].

IC₅₀ & Target

STING^[1]

In Vitro

2’3’-c-di-AM(PS)2 (Rp,Rp) is unstable in its free base form. 2’3’-c-di-AM(PS)2 (Rp,Rp) ammonium salt (HY-12885B) improves both stability and lipophilicity, promoting significantly increased STING signaling as compared to endogenous and pathogen-derived cyclic dinucleotides (CDNs)^[1].
2’3’-c-di-AM(PS)2 (Rp,Rp) shows enhanced type I IFN production over CDA in THP-1 human monocytes. In contrast, the dithio, mixed-linkage CDN derivatives (ML RR-CDA, ML RR-S2 CDG, and ML RR-S2 cGAMP) potently activate all five hSTING alleles, including the refractory hSTING^REF and hSTING^Q alleles. 2’3’-c-di-AM(PS)2 (Rp,Rp) induces the highest expression of IFN-β and the pro-inflammatory cytokines TNF-α, IL-6, and MCP-1 on a molar equivalent basis, as compared to endogenous ML cGAMP and the TLR3 agonist poly I:C. 2’3’-c-di-AM(PS)2 (Rp,Rp) is also found to induce aggregation of STING and induce phosphorylation of TBK1 and IRF3 in mouse bone marrow macrophage (BMM). 2’3’-c-di-AM(PS)2 (Rp,Rp) induces significantly higher levels of IFN-α when compared to ML cGAMP^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

2’3’-c-di-AM(PS)2 (Rp,Rp) shows higher anti-tumor control than the endogenous ML cGAMP. A dose response of the 2’3’-c-di-AM(PS)2 (Rp,Rp) compound is performed in B16 tumor-bearing mice, which identifies an optimal antitumor dose level that also elicites maximum tumor antigen-specific CD8⁺ T cell responses, and improves long-term survival to 50%^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

690.54

Formula

C₂₀H₂₄N₁₀O₁₀P₂S₂

CAS No.

1638241-89-0

Appearance

Solid

Color

White to off-white

SMILES

O[C@H]1[C@@H](O[P@@](OC[C@H]2O[C@@H](N3C(N=CN=C4N)=C4N=C3)[C@H](O)[C@@H]2O5)(S)=O)[C@H](N6C(N=CN=C7N)=C7N=C6)O[C@@H]1CO[P@]5(S)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

H₂O : 20 mg/mL (28.96 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.4481 mL	7.2407 mL	14.4814 mL
5 mM	0.2896 mL	1.4481 mL	2.8963 mL
10 mM	0.1448 mL	0.7241 mL	1.4481 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

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Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.85%

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Data Sheet (282 KB) SDS (394 KB)

COA (258 KB) HNMR (487 KB) LCMS (95 KB)

Handling Instructions (2659 KB)

References

[1]. Corrales L, et al. Direct Activation of STING in the Tumor Microenvironment Leads to Potent and Systemic Tumor Regression and Immunity. Cell Rep. 2015 May 19;11(7):1018-30. [Content Brief]

Cell Assay ^[1]	Cryopreserved hPBMCs are thawed and 1×10⁶ cells per well are plated in a 96 well plate in RPMI media. Cells are stimulated with 10 μM ADU-S100 or ML cGAMP for 6 hours and supernatants are harvested. Supernatants are diluted 1:2 and assayed for IFN-α protein using Cytometric Bead Array (CBA) Human Flex Set. Data is collected using a FACSVerse cytometer and analyzed by FCAP Array Software^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[1]	Mice^[1] WT C57BL/6 mice are inoculated with 5×10⁴ B16.F10 cells in the left flank (n=8). When tumor volumes are 100 mm³ mice receive three IT doses of either ML RR-S2 CDG (25 μg), ADU-S100 (50 μg), or HBSS as control. WT C57BL/6 mice are inoculated with 5×10⁴ B16.F10 cells in the left flank (n=5). When tumor volumes are 100 mm³ they received three IT doses of ADU-S100 at 5, 25, 50 or 100 μg or HBSS as control. WT C57BL/6 mice are inoculated with 5×10⁴ B16.F10 cells in the left flank (n=8). When tumor volumes are 100 mm³ they receive three IT doses of 100 μg ADU-S100 or HBSS as control. Treatments are administered on days 13, 17 and 20 and tumor measurements are taken twice weekly. Results are shown as percent survival by Log-rank (Mantel-Cox) test (A and C)^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Corrales L, et al. Direct Activation of STING in the Tumor Microenvironment Leads to Potent and Systemic Tumor Regression and Immunity. Cell Rep. 2015 May 19;11(7):1018-30. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	1.4481 mL	7.2407 mL	14.4814 mL	36.2036 mL
	5 mM	0.2896 mL	1.4481 mL	2.8963 mL	7.2407 mL
	10 mM	0.1448 mL	0.7241 mL	1.4481 mL	3.6204 mL
	15 mM	0.0965 mL	0.4827 mL	0.9654 mL	2.4136 mL
	20 mM	0.0724 mL	0.3620 mL	0.7241 mL	1.8102 mL
	25 mM	0.0579 mL	0.2896 mL	0.5793 mL	1.4481 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

2’3’-c-di-AM(PS)2 (Rp,Rp) Related Classifications

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Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

2’3’-c-di-AM(PS)2 (Rp,Rp)1638241-89-0ADU-S100 MIW815 ML RR-S2 CDAMIW815MIW 815MIW-815STINGStimulator of Interferon GenesTMEM173MITAERISMPYSInhibitorinhibitorinhibit

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2’3’-c-di-AM(PS)2 (Rp,Rp) (Synonyms: ADU-S100; MIW815; ML RR-S2 CDA)

Customer Review

Other Forms of 2’3’-c-di-AM(PS)2 (Rp,Rp):

2’3’-c-di-AM(PS)2 (Rp,Rp) Related Antibodies

87 Publications Citing Use of MCE 2’3’-c-di-AM(PS)2 (Rp,Rp)

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Biological Activity

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References

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2’3’-c-di-AM(PS)2 (Rp,Rp) Related Antibodies

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Complete Stock Solution Preparation Table

2’3’-c-di-AM(PS)2 (Rp,Rp) Related Classifications

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