cGAMP (Synonyms: Cyclic GMP-AMP; 3',3'-cGAMP)

Name: cGAMP
Brand: MedChemExpress
SKU: HY-12512
Rating: 5.0 (26 reviews)

Cat. No.: HY-12512 Purity: 98.47%: Data Sheet
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cGAMP (Cyclic GMP-AMP) functions as an endogenous second messenger in metazoans and triggers interferon production in response to cytosolic DNA. cGAMP activates stimulator of interferon genes (STING), which activates a signaling cascade leading to the production of type I interferons and other immune mediators.

For research use only. We do not sell to patients.

CAS No. : 849214-04-6

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Solid
500 μg	In-stock	Estimated Time of Arrival: December 31
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 26 publication(s) in Google Scholar

Other Forms of cGAMP:

cGAMP disodium In-stock
cGAMP diammonium In-stock

26 Publications Citing Use of MCE cGAMP

Cell Imaging/Staining

RT-PCR

Bio/Physico-chemical Assay

: cGAMP purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2025 Oct 1;10(1):321. [Abstract]; cGAMP accelerated calcium deposition in VSMCs.

: cGAMP purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2025 Oct 1;10(1):321. [Abstract]; cGAMP increased the calcium content in VSMCs.

: cGAMP purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Dec 4;15(1):10543. [Abstract]; cGAMP (2.5 or 10 μM, 24 h) increased OLFM4 protein expression in HGC-27 cells.

: cGAMP purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Dec 4;15(1):10543. [Abstract]; cGAMP (10 μM, 6, 12, and 24 h) increased OLFM4 mRNA expression in HGC-27 cells.

: cGAMP purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2025 Jun;32(6):1167-1179. [Abstract]; cGAMP (0, 2, and 200 nM; 3 or 6 h) increased STING phosphorylation at 3 h with 200 nM, but not at 6 h in THP-1 cells.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
H69AR	EC₅₀	4.74 μM Compound: 3',3'-cGAMP	Activation of wild-type human STING expressed in human THP1-Dual cells co-expressing IRF3-activated ISRE assessed as IRF3 reporter activation measured after 7 hrs by luciferase reporter gene assay Activation of wild-type human STING expressed in human THP1-Dual cells co-expressing IRF3-activated ISRE assessed as IRF3 reporter activation measured after 7 hrs by luciferase reporter gene assay	[PMID: 34019405]
H69AR	EC₅₀	58.46 μM Compound: 3',3'-cGAMP	Activation of wild-type human STING expressed in digitonin treated human THP1-Dual cells co-expressing IRF3-activated ISRE assessed as IRF3 reporter activation measured after 30 mins by luciferase reporter gene assay Activation of wild-type human STING expressed in digitonin treated human THP1-Dual cells co-expressing IRF3-activated ISRE assessed as IRF3 reporter activation measured after 30 mins by luciferase reporter gene assay	[PMID: 34019405]
L929	EC₅₀	20 nM Compound: 3',3'-cGAMP	Inhibition of STING in mouse L929 cells assessed as reduction in cGAMP induce IFNbeta expression measured after 4 hrs by qRT-PCR Inhibition of STING in mouse L929 cells assessed as reduction in cGAMP induce IFNbeta expression measured after 4 hrs by qRT-PCR	[PMID: 32206239]
PBMC	EC₅₀	15.14 μM Compound: 3',3'-cGAMP	Induction of INF-gamma production in healthy human PBMC incubated for 16 hrs Induction of INF-gamma production in healthy human PBMC incubated for 16 hrs	[PMID: 34019405]
PBMC	EC₅₀	17.25 μM Compound: 3'3'-cGAMP	Induction of IFNgamma secretion in human PBMC incubated for 16 hrs Induction of IFNgamma secretion in human PBMC incubated for 16 hrs	[PMID: 36201304]
PBMC	EC₅₀	18.72 μM Compound: 3'3'-cGAMP	Induction of TNFalpha secretion in human PBMC incubated for 16 hrs Induction of TNFalpha secretion in human PBMC incubated for 16 hrs	[PMID: 36201304]
PBMC	EC₅₀	26.71 μM Compound: 3',3'-cGAMP	Induction of INF-alpha production in healthy human PBMC incubated for 16 hrs Induction of INF-alpha production in healthy human PBMC incubated for 16 hrs	[PMID: 34019405]
PBMC	EC₅₀	7.65 μM Compound: 3'3'-cGAMP	Induction of IFNalpha secretion in human PBMC incubated for 16 hrs Induction of IFNalpha secretion in human PBMC incubated for 16 hrs	[PMID: 36201304]
PBMC	EC₅₀	> 300 μM Compound: 3',3'-cGAMP	Induction of TNF-alpha production in healthy human PBMC incubated for 16 hrs Induction of TNF-alpha production in healthy human PBMC incubated for 16 hrs	[PMID: 34019405]

In Vitro

cGAMP promotes the antigen-specific proliferation capacity of spleen cells in mice^[2].
cGAMP directly activates murine and human dendritic cells in vitro^[2].
On stimulation with cGAMP, fibroblasts from the patients showed increased transcription of IFNB1 but not of the genes encoding interleukin-1 (IL1), interleukin-6 (IL6), or tumor necrosis factor (TNF)^[3].
cGAMP activates the endoplasmic reticulum (ER)-resident receptor STING, thereby inducing an antiviral state and the secretion of type I IFNs^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

cGAMP (5 μg; nostril mucosal adjuvant) promotes the antigen-specific cytokine production by spleen cells of immunized mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female C57BL/6 (H-2b) mice 6–8 weeks old^[2]
Dosage:	5 µg
Administration:	Nostril mucosal adjuvant
Result:	Higher titers of ovalbumin (OVA)-specific IgA and total IgG as well as IgG1 and IgG2c in the sera of mice immunized with cGAMP-adjuvanted OVA as compared to sera from OVA-immunized mice.

Molecular Weight

674.41

Formula

C₂₀H₂₄N₁₀O₁₃P₂

CAS No.

849214-04-6

Appearance

Solid

Color

White to yellow

SMILES

O=P(O[C@H]1[C@@H](O)[C@H](N2C=NC3=C2N=CN=C3N)O[C@@H]1COP4(O)=O)(O)OC[C@@H]5[C@@H](O4)[C@@H](O)[C@H](N6C=NC7=C6N=C(N)NC7=O)O5

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

H₂O : 180 mg/mL (266.90 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.4828 mL	7.4139 mL	14.8278 mL
5 mM	0.2966 mL	1.4828 mL	2.9656 mL
10 mM	0.1483 mL	0.7414 mL	1.4828 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

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Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.22%

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Data Sheet (278 KB) SDS (393 KB)

COA (252 KB) HNMR (258 KB) RP-HPLC (243 KB) MS (127 KB)

Handling Instructions (2659 KB)

References

[1]. Wu J, et al. Cyclic GMP-AMP is an endogenous second messenger in innate immune signaling by cytosolic DNA. Science. 2013 Feb 15;339(6121):826-30. [Content Brief]

[2]. Skrnjug I, et al. Cyclic GMP-AMP displays mucosal adjuvant activity in mice. PLoS One. 2014 Oct 8;9(10):e110150. [Content Brief]

[3]. Liu Y, et al. Activated STING in a vascular and pulmonary syndrome. N Engl J Med. 2014 Aug 7;371(6):507-18. [Content Brief]

[4]. Ablasser A, et al. Cell intrinsic immunity spreads to bystander cells via the intercellular transfer of cGAMP. Nature. 2013 Nov 28;503(7477):530-4. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	1.4828 mL	7.4139 mL	14.8278 mL	37.0694 mL
	5 mM	0.2966 mL	1.4828 mL	2.9656 mL	7.4139 mL
	10 mM	0.1483 mL	0.7414 mL	1.4828 mL	3.7069 mL
	15 mM	0.0989 mL	0.4943 mL	0.9885 mL	2.4713 mL
	20 mM	0.0741 mL	0.3707 mL	0.7414 mL	1.8535 mL
	25 mM	0.0593 mL	0.2966 mL	0.5931 mL	1.4828 mL
	30 mM	0.0494 mL	0.2471 mL	0.4943 mL	1.2356 mL
	40 mM	0.0371 mL	0.1853 mL	0.3707 mL	0.9267 mL
	50 mM	0.0297 mL	0.1483 mL	0.2966 mL	0.7414 mL
	60 mM	0.0247 mL	0.1236 mL	0.2471 mL	0.6178 mL
	80 mM	0.0185 mL	0.0927 mL	0.1853 mL	0.4634 mL
	100 mM	0.0148 mL	0.0741 mL	0.1483 mL	0.3707 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

cGAMP Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

cGAMP849214-04-6Cyclic GMP-AMP 3',3'-cGAMPSTINGStimulator of Interferon GenesTMEM173MITAERISMPYSsecond messengerantigencytosolic DNAinterferonsimmune mediatorsInhibitorinhibitorinhibit

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cGAMP (Synonyms: Cyclic GMP-AMP; 3',3'-cGAMP)

Customer Review

Other Forms of cGAMP:

cGAMP Related Antibodies

26 Publications Citing Use of MCE cGAMP

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

cGAMP Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

cGAMP Related Classifications

Keywords:

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Customer Review

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