2’3’-c-di-AM(PS)2 (Rp,Rp) disodium salt (Synonyms: ADU-S100 disodium salt; MIW815 disodium salt; ML RR-S2 CDA disodium salt)

Name: 2’3’-c-di-AM(PS)2 (Rp,Rp) disodium salt
Brand: MedChemExpress
SKU: HY-12885A
Rating: 5.0 (87 reviews)

Cat. No.: HY-12885A Purity: 99.46%: Data Sheet
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2’3’-c-di-AM(PS)2 (Rp,Rp) disodium salt (ADU-S100 disodium salt) is an activator of stimulator of interferon genes (STING).

For research use only. We do not sell to patients.

CAS No. : 1638750-95-4

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
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Based on 87 publication(s) in Google Scholar

Other Forms of 2’3’-c-di-AM(PS)2 (Rp,Rp) disodium salt:

2’3’-c-di-AM(PS)2 (Rp,Rp) enantiomer ammonium salt In-stock
2’3’-c-di-AM(PS)2 (Rp,Rp) In-stock
2’3’-c-di-AM(PS)2 (Rp,Rp) ammonium salt In-stock

87 Publications Citing Use of MCE 2’3’-c-di-AM(PS)2 (Rp,Rp) disodium salt

Cell Proliferation/Viability Assay

In Vivo Efficacy Study

IHC

Flow Cytometry

RT-PCR

: 2’3’-c-di-AM(PS)2 (Rp,Rp) disodium salt purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2025 Oct;26(10):1766-1780. [Abstract]; 2’3’-c-di-AM(PS)2 (Rp,Rp) (ADU-S100) (10 μM) was added to the culture of KPC cells or endothelial cells (HUVEC) for indicated time, and the activation of STING signaling was analyzed by IRF3 Ser385 phosphorylation. The quantification of phosphorylated IRF3 was normalized by total IRF3. β-actin was used as a loading control.

: 2’3’-c-di-AM(PS)2 (Rp,Rp) disodium salt purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2025 Oct;26(10):1766-1780. [Abstract]; Leukocytes were isolated from peripheral blood or the peritoneum of normal mice and incubated in vitro with 7.2 μg/mL STING agonist 2’3’-c-di-AM(PS)2 (Rp,Rp) (ADU-S100) for 2 h at 37 °C. Phosphorylation of IRF3 in leukocyte subsets (CD11b⁺ myeloid cells, CD3⁺ cells, CD19⁺ cells, and NK1.1⁺ cells) was detected by α-phospho-IRF3 Ser385 antibody staining. Cells were permeabilized using eBioscience™ Transcription Factor Staining Buffer Set before staining. The percent fraction of phospho-IRF3⁺ cells in each subset of blood or peritoneal leukocytes was determined by flow cytometry.

: 2’3’-c-di-AM(PS)2 (Rp,Rp) disodium salt purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2025 Oct;26(10):1766-1780. [Abstract]; STING agonist 2’3’-c-di-AM(PS)2 (Rp,Rp) (ADU-S100) (2 μg intratumoral) and/or anti-LTβR agonistic antibody (100 μg, intraperitoneal (i.p.)) were administered to mice bearing subcutaneous KPC tumors as monotherapy or in combination as indicated. Immunohistochemistry of B cells (CD19, brown) and HEVs (MECA-79, magenta) shows numerous TLS induced by combination therapy (day 14). Scale bars, 1 mm (low magnification) and 100 μm (high magnification).

: 2’3’-c-di-AM(PS)2 (Rp,Rp) disodium salt purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Apr;12(16):e2414110. [Abstract]; The relative mRNA expression of Ifnβ1 in 4T1 cells, MC38 cells and B16F10 cells after treatment with TACTIC, CS or ADU‐S100 (2’3’-c-di-AM(PS)2 (Rp,Rp) disodium salt, 20 µg/mL; 24 h) (n = 3).

: 2’3’-c-di-AM(PS)2 (Rp,Rp) disodium salt purchased from MedChemExpress. Usage Cited in: Mol Ther. 2025 Sep 22:S1525-0016(25)00761-0. [Abstract]; Titration of the agonist ADU-S100 in B16-OVA tumor model. OVA mRNA, 10 μg; C1, 1.6 mg; ADU-S100 (2’3’-c-di-AM(PS)2 (Rp,Rp) disodium salt): 10/20/40 μg. n=5 mice per group. The results indicated that the ADU-S100 dosage used (20 μg/mice) was likely optimal for the C1-mRNA+ADU-S100(nano) cancer vaccine.

: 2’3’-c-di-AM(PS)2 (Rp,Rp) disodium salt purchased from MedChemExpress. Usage Cited in: Mol Ther. 2025 Sep 22:S1525-0016(25)00761-0. [Abstract]; Representative images of tumor-infiltrating CD8⁺ T cells by immunohistochemistry (left) and quantification of CD8⁺ T cells (right). Scale bars: 100 μm (upper) and 50 μm (lower). Tumor-infiltrating immune cell analysis by immunohistochemistry demonstrated that CD8⁺ T cells were enriched in tumors from Sting^f/f Itgax-Cre^– mice immunized with C1-OVA mRNA+ADU-S100(nano), but not in tumors from Sting^f/f Itgax-Cre⁺ mice. The doses of mRNA vaccine per mouse in vivo were as follows: mRNA, 10 μg; C1, 1.6 mg; ADU-S100, 20 μg; c-di-AMP, 20 μg. C1-mRNA + ADU-S100(nano) was prepared by encapsulating ADU-S100 into the C1-mRNA nanovaccine during nanoparticle preparation.

: 2’3’-c-di-AM(PS)2 (Rp,Rp) disodium salt purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2024 May 13;42(5):850-868.e9. [Abstract]; Cell viability assays of isogenic NTC, Trp53 KO, and Bak/Bax KO Eμ-Myc lymphoma cell lines treated with S63845 in combination with ADU-S100 (2’3’-c-di-AM(PS)2 (Rp,Rp) disodium salt, 2-10 μg/mL), MSA-2 (12-34 μM), or diABZI for 24 h.

: 2’3’-c-di-AM(PS)2 (Rp,Rp) disodium salt purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2024 May 13;42(5):850-868.e9. [Abstract]; RT-qPCR for BH3-only and STING target genes in three independent isogenic NTC and Trp53 KO Em-Myc lymphoma cell lines treated with ADU-S100 (2’3’-c-di-AM(PS)2 (Rp,Rp) disodium salt, 10 μg/mL) for 24 h.Fold change is relative to the AH15A DMSO-treated NTC sample

: 2’3’-c-di-AM(PS)2 (Rp,Rp) disodium salt purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2024 May 13;42(5):850-868.e9. [Abstract]; Cell viability assays of Eμ-Myc lymphoma cell lines treated with ADU-S100 (HY-12885A; AH15A/560: 0.5 μg/mL; 47A: 5 μg/mL) and/or TPCA-1 (HY-10074) for 24 h. Error bars represent SD for three independent experiments. Diagram illustrates how TPCA-1 acts to inhibit NF-κB signaling.

: 2’3’-c-di-AM(PS)2 (Rp,Rp) disodium salt purchased from MedChemExpress. Usage Cited in: Nat Cell Biol. 2024 Sep;26(9):1585-1596. [Abstract]; Cell viability assays measuring 5-FU response in cGAS knockdown HCT116 cells stably expressing shRNA-resistant cGAS-S213D mutants or pretreated with 10 µM 2’3’-c-di-AM(PS)2 (Rp,Rp) (ADU-S100) for 6 hours.

: 2’3’-c-di-AM(PS)2 (Rp,Rp) disodium salt purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Mar 13;14(1):1390. [Abstract]; ADU-S100 (10 μM; 48 h; labeled as STING) enhances type I interferon gene expression in human dendritic cells (DCs).

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Description

2’3’-c-di-AM(PS)2 (Rp,Rp) disodium salt (ADU-S100 disodium salt) is an activator of stimulator of interferon genes (STING).

IC₅₀ & Target

STING^[1]

In Vitro

2’3’-c-di-AM(PS)2 (Rp,Rp) shows enhanced type I IFN production over CDA in THP-1 human monocytes. In contrast, the dithio, mixed-linkage cyclic dinucleotide (CDN) derivatives (ML RR-CDA, ML RR-S2 CDG, and ML RR-S2 cGAMP) potently activate all five hSTING alleles, including the refractory hSTING^REF and hSTING^Q alleles. 2’3’-c-di-AM(PS)2 (Rp,Rp) induces the highest expression of IFN-β and the pro-inflammatory cytokines TNF-α, IL-6, and MCP-1 on a molar equivalent basis, as compared to endogenous ML cGAMP and the TLR3 agonist poly I:C. 2’3’-c-di-AM(PS)2 (Rp,Rp) is also found to induce aggregation of STING and induce phosphorylation of TBK1 and IRF3 in mouse bone marrow macrophage (BMM). 2’3’-c-di-AM(PS)2 (Rp,Rp) induces significantly higher levels of IFN-α when compared to ML cGAMP^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

2’3’-c-di-AM(PS)2 (Rp,Rp) shows higher anti-tumor control than the endogenous ML cGAMP. A dose response of the 2’3’-c-di-AM(PS)2 (Rp,Rp) compound is performed in B16 tumor-bearing mice, which identifies an optimal antitumor dose level that also elicites maximum tumor antigen-specific CD8⁺ T cell responses, and improves long-term survival to 50%^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

734.51

Formula

C₂₀H₂₂N₁₀Na₂O₁₀P₂S₂

CAS No.

1638750-95-4

Appearance

Solid

Color

White to off-white

SMILES

O[C@H]1[C@@H](O[P@@](OC[C@H]2O[C@@H](N3C(N=CN=C4N)=C4N=C3)[C@H](O)[C@@H]2O5)(S[Na])=O)[C@H](N6C(N=CN=C7N)=C7N=C6)O[C@@H]1CO[P@]5(S[Na])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (136.15 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.3615 mL	6.8073 mL	13.6145 mL
5 mM	0.2723 mL	1.3615 mL	2.7229 mL
10 mM	0.1361 mL	0.6807 mL	1.3615 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: ≥ 25 mg/mL (34.04 mM); Clear solution

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.91%

Select Batch:

Data Sheet (283 KB) SDS (394 KB)

COA (259 KB) HNMR (250 KB) CNMR (196 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Corrales L, et al. Direct Activation of STING in the Tumor Microenvironment Leads to Potent and Systemic Tumor Regression and Immunity. Cell Rep. 2015 May 19;11(7):1018-30. [Content Brief]

Cell Assay ^[1]	Cryopreserved hPBMCs are thawed and 1×10⁶ cells per well are plated in a 96 well plate in RPMI media supplemented with 10% FBS, 1% non-essential amino acids, 1% penicillin/streptomycin, L-glutamine, 10 mM HEPES buffer, 1 mM Sodium Pyruvate, 0.055 mM β-ME at 37°C with 5% CO₂. Cells are stimulated with 10 μM ADU-S100 or ML cGAMP for 6 hours and supernatants are harvested. Supernatants are diluted 1:2 and assayed for IFN-α protein using Cytometric Bead Array (CBA) Human Flex Set. Data is collected using a FACSVerse cytometer and analyzed by FCAP Array Software^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[1]	Mice^[1] WT C57BL/6 mice are inoculated with 5×10⁴ B16.F10 cells in the left flank (n=8). When tumor volumes are 100 mm³ mice receive three IT doses of either ML RR-S2 CDG (25 μg), ADU-S100 (50 μg), or HBSS as control. WT C57BL/6 mice are inoculated with 5×10⁴ B16.F10 cells in the left flank (n=5). When tumor volumes are 100 mm³ they received three IT doses of ADU-S100 at 5, 25, 50 or 100 μg or HBSS as control. WT C57BL/6 mice are inoculated with 5×10⁴ B16.F10 cells in the left flank (n=8). When tumor volumes are 100 mm³ they receive three IT doses of 100 μg ADU-S100 or HBSS as control. Treatments are administered on days 13, 17 and 20 and tumor measurements are taken twice weekly. Results are shown as percent survival by Log-rank (Mantel-Cox) test (A and C)^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Corrales L, et al. Direct Activation of STING in the Tumor Microenvironment Leads to Potent and Systemic Tumor Regression and Immunity. Cell Rep. 2015 May 19;11(7):1018-30. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	1.3615 mL	6.8073 mL	13.6145 mL	34.0363 mL
	5 mM	0.2723 mL	1.3615 mL	2.7229 mL	6.8073 mL
	10 mM	0.1361 mL	0.6807 mL	1.3615 mL	3.4036 mL
	15 mM	0.0908 mL	0.4538 mL	0.9076 mL	2.2691 mL
	20 mM	0.0681 mL	0.3404 mL	0.6807 mL	1.7018 mL
	25 mM	0.0545 mL	0.2723 mL	0.5446 mL	1.3615 mL
	30 mM	0.0454 mL	0.2269 mL	0.4538 mL	1.1345 mL
	40 mM	0.0340 mL	0.1702 mL	0.3404 mL	0.8509 mL
	50 mM	0.0272 mL	0.1361 mL	0.2723 mL	0.6807 mL
	60 mM	0.0227 mL	0.1135 mL	0.2269 mL	0.5673 mL
	80 mM	0.0170 mL	0.0851 mL	0.1702 mL	0.4255 mL
	100 mM	0.0136 mL	0.0681 mL	0.1361 mL	0.3404 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

2’3’-c-di-AM(PS)2 (Rp,Rp) disodium salt Related Classifications

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Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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2’3’-c-di-AM(PS)2 (Rp,Rp) disodium1638750-95-4ADU-S100 disodium MIW815 disodium ML RR-S2 CDA disodiumSTINGStimulator of Interferon GenesTMEM173MITAERISMPYSInhibitorinhibitorinhibit

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2’3’-c-di-AM(PS)2 (Rp,Rp) disodium salt (Synonyms: ADU-S100 disodium salt; MIW815 disodium salt; ML RR-S2 CDA disodium salt)

Customer Review

Other Forms of 2’3’-c-di-AM(PS)2 (Rp,Rp) disodium salt:

2’3’-c-di-AM(PS)2 (Rp,Rp) disodium salt Related Antibodies

87 Publications Citing Use of MCE 2’3’-c-di-AM(PS)2 (Rp,Rp) disodium salt

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•Related Screening Libraries:

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Biological Activity

Protocol

Purity & Documentation

References

Customer Review

2’3’-c-di-AM(PS)2 (Rp,Rp) disodium salt Related Antibodies

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Complete Stock Solution Preparation Table

2’3’-c-di-AM(PS)2 (Rp,Rp) disodium salt Related Classifications

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