PD150606

Name: PD150606
Brand: MedChemExpress
SKU: HY-100529
Rating: 5.0 (14 reviews)

Cat. No.: HY-100529 Purity: 98.61%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

PD 150606 is a selective, cell-permeable non-peptide calpain inhibitor with K_i values of 0.21 μM and 0.37 μM for μ- and m-calpains respectively, which is neuroprotective.

For research use only. We do not sell to patients.

CAS No. : 179528-45-1

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 14 publication(s) in Google Scholar

Other Forms of PD150606:

PD150606 (Standard) Get quote

14 Publications Citing Use of MCE PD150606

Cell Imaging/Staining

: PD150606 purchased from MedChemExpress. Usage Cited in: Mol Cancer. 2024 Sep 30;23(1):215. [Abstract]; Western blot analysis of RXRα expression after 24 h of treatment with PD150606 (200 nM, m-calpain inhibitor) or ZFY-CHO (10 µM, CTSL inhibitor).

: PD150606 purchased from MedChemExpress. Usage Cited in: Cell Rep. 2023 Dec 2;42(12):113522. [Abstract]; Representative immunofluorescence images of DRG neurons seeded on laminin-coated glass slides and treated with culture medium (control), 10 μM PD150606 or 30 μM Yoda1 for 24 hours.

: PD150606 purchased from MedChemExpress. Usage Cited in: Cell Rep. 2023 Dec 2;42(12):113522. [Abstract]; Western blots of integrin-β1, SN25, and GAPDH in DRG neurons seeded on glass slides and treated with 10 μM PD150606 for 30 min at DIV1.

: PD150606 purchased from MedChemExpress. Usage Cited in: Cell Rep. 2023 Dec 2;42(12):113522. [Abstract]; Representative images were cultured on substrates at 5.0 and 20 kPa and incubated for 24 hours with medium (control) or 10 μM PD150606.

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Biological Activity
Purity & Documentation
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Description

PD 150606 is a selective, cell-permeable non-peptide calpain inhibitor with K_i values of 0.21 μM and 0.37 μM for μ- and m-calpains respectively, which is neuroprotective^[1].

IC₅₀ & Target

Ki: 0.21 μM (μ-calpains), 0.37 μM (m-calpains)^[1]

In Vitro

PD150606 interacts with both calcium-binding domains (μ- and m-calpains) of Calpain^[1].
PD150606 attenuates hypoxic/hypoglycemic injury to cerebrocortical neurons in culture and excitotoxic injury to Purkinje cells in cerebellar slices^[1].
PD 150606 (25 μM; 0-12 hours) reduces Cycloheximide (10 mg/ml) -triggered apoptosis of neutrophils^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

306.12

Formula

C₉H₇IO₂S

CAS No.

179528-45-1

Appearance

Solid

Color

Off-white to yellow

SMILES

O=C(O)/C(S)=C/C1=CC=C(I)C=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : ≥ 28 mg/mL (91.47 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2667 mL	16.3335 mL	32.6669 mL
5 mM	0.6533 mL	3.2667 mL	6.5334 mL
10 mM	0.3267 mL	1.6333 mL	3.2667 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.17 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (8.17 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.17 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.61%

Select Batch:

Data Sheet (273 KB) SDS (641 KB)

COA (246 KB) HNMR (233 KB) RP-HPLC (247 KB)

Handling Instructions (2659 KB)

References

[1]. 1. Wang KK et al. An alpha-mercaptoacrylic acid derivative is a selective nonpeptide cell-permeable calpain inhibitor and is neuroprotective. Proc Natl Acad Sci U S A. 1996 Jun 25;93(13):6687-92. [Content Brief]

[2]. 2. Squier MK , et al. Calpain and calpastatin regulate neutrophil apoptosis. J Cell Physiol. 1999 Mar;178(3):311-9. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.2667 mL	16.3335 mL	32.6669 mL	81.6673 mL
	5 mM	0.6533 mL	3.2667 mL	6.5334 mL	16.3335 mL
	10 mM	0.3267 mL	1.6333 mL	3.2667 mL	8.1667 mL
	15 mM	0.2178 mL	1.0889 mL	2.1778 mL	5.4445 mL
	20 mM	0.1633 mL	0.8167 mL	1.6333 mL	4.0834 mL
	25 mM	0.1307 mL	0.6533 mL	1.3067 mL	3.2667 mL
	30 mM	0.1089 mL	0.5444 mL	1.0889 mL	2.7222 mL
	40 mM	0.0817 mL	0.4083 mL	0.8167 mL	2.0417 mL
	50 mM	0.0653 mL	0.3267 mL	0.6533 mL	1.6333 mL
	60 mM	0.0544 mL	0.2722 mL	0.5444 mL	1.3611 mL
	80 mM	0.0408 mL	0.2042 mL	0.4083 mL	1.0208 mL

PD150606 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PD150606179528-45-1PD 150606PD-150606ProteasomeInhibitorinhibitorinhibit

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PD150606

Customer Review

Other Forms of PD150606:

PD150606 Related Antibodies

14 Publications Citing Use of MCE PD150606

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

PD150606 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

PD150606 Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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