2. Cell Cycle/DNA Damage Anti-infection
  3. Nucleoside Antimetabolite/Analog HIV
  4. Pseudothymidine

Pseudothymidine  (Synonyms: 5-Methyl-2'-Deoxypseudouridin)

Cat. No.: HY-101969 Purity: 99.44%
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Pseudothymidine is a C-nucleoside analog of thymidine.

For research use only. We do not sell to patients.

Pseudothymidine Chemical Structure

Pseudothymidine Chemical Structure

CAS No. : 65358-15-8

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 605 In-stock
Solution
10 mM * 1 mL in DMSO USD 605 In-stock
Solid
1 mg USD 185 In-stock
5 mg USD 550 In-stock
10 mg USD 880 In-stock
25 mg USD 1750 In-stock
50 mg USD 2800 In-stock
100 mg USD 4480 In-stock
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Based on 1 publication(s) in Google Scholar

Pseudothymidine Related Antibodies

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  • Biological Activity

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  • Purity & Documentation

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Description

Pseudothymidine is a C-nucleoside analog of thymidine.

In Vitro

Pseudothymidine is a C-nucleoside analog of thymidine[1]. The calculated ΔΔG°50/mod is -0.5 kcal/mol, with a ΔTm/mod of 0.82°C. For the duplexes containing nine dA-T/ψT pairs, the ΔTm/mod is -0.9°C and a ΔΔG°50/mod is +1.1 kcal/mol. The modification of the duplex containing 12 consecutive dA-T/ψT base pairs produces a ΔTm/mod of -0.9°C and a ΔΔG°50/mod of +1.2 kcal/mol[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Pseudothymidine Related Antibodies
Molecular Weight

242.23

Formula

C10H14N2O5
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(NC1=O)N(C)C=C1[C@H]2C[C@H](O)[C@@H](CO)O2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 61.17 mg/mL (252.53 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.1283 mL 20.6415 mL 41.2831 mL
5 mM 0.8257 mL 4.1283 mL 8.2566 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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Volume (start)

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C2

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V2

In Vivo Dissolution Calculator
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References
Kinase Assay

Thermal DNA duplex denaturation studies are performed with templates containing up to twelve consecutive dA residues that are paired with its complement template containing consecutive T or Pseudothymidine (ψT) residues. Experiments are performed in a buffer (45 mM NaCl, 45 mM sodium citrate, pH 8.1, final vol. 1.5 mL) containing template and its complement (1.5 μM of each). Absorbance (260 nm) is monitored over a range of 25.0 to 90.0°C with a change in temperature of 0.5°C/min for five heating cycles. The initial heating cycle is discarded and the Tm is determined by averaging the temperatures of the remaining four cycles. The ΔTm between similar duplexes is calculated by subtracting the Tm of the duplex containing standard bases from the Tm of the duplex containing C-glycosides (including Pseudothymidine)[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.1283 mL 20.6415 mL 41.2831 mL 103.2077 mL
5 mM 0.8257 mL 4.1283 mL 8.2566 mL 20.6415 mL
10 mM 0.4128 mL 2.0642 mL 4.1283 mL 10.3208 mL
15 mM 0.2752 mL 1.3761 mL 2.7522 mL 6.8805 mL
20 mM 0.2064 mL 1.0321 mL 2.0642 mL 5.1604 mL
25 mM 0.1651 mL 0.8257 mL 1.6513 mL 4.1283 mL
30 mM 0.1376 mL 0.6881 mL 1.3761 mL 3.4403 mL
40 mM 0.1032 mL 0.5160 mL 1.0321 mL 2.5802 mL
50 mM 0.0826 mL 0.4128 mL 0.8257 mL 2.0642 mL
60 mM 0.0688 mL 0.3440 mL 0.6881 mL 1.7201 mL
80 mM 0.0516 mL 0.2580 mL 0.5160 mL 1.2901 mL
100 mM 0.0413 mL 0.2064 mL 0.4128 mL 1.0321 mL
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Pseudothymidine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Pseudothymidine65358-15-85-Methyl-2'-DeoxypseudouridinNucleoside Antimetabolite/AnalogHIVHuman immunodeficiency virusInhibitorinhibitorinhibit

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