1. Cell Cycle/DNA Damage
  2. Nucleoside Antimetabolite/Analog

Pseudothymidine (Synonyms: 5-Methyl-2'-Deoxypseudouridin)

Cat. No.: HY-101969 Purity: 99.85%
Handling Instructions

Pseudothymidine is a C-nucleoside analog of thymidine.

For research use only. We do not sell to patients.

Pseudothymidine Chemical Structure

Pseudothymidine Chemical Structure

CAS No. : 65358-15-8

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 608 In-stock
Estimated Time of Arrival: December 31
1 mg USD 228 In-stock
Estimated Time of Arrival: December 31
5 mg USD 780 In-stock
Estimated Time of Arrival: December 31
10 mg USD 1140 In-stock
Estimated Time of Arrival: December 31
25 mg USD 2340 In-stock
Estimated Time of Arrival: December 31
50 mg USD 3540 In-stock
Estimated Time of Arrival: December 31
100 mg USD 5640 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Pseudothymidine is a C-nucleoside analog of thymidine.

In Vitro

Pseudothymidine is a C-nucleoside analog of thymidine[1]. The calculated ΔΔG°50/mod is -0.5 kcal/mol, with a ΔTm/mod of 0.82°C. For the duplexes containing nine dA-T/ψT pairs, the ΔTm/mod is -0.9°C and a ΔΔG°50/mod is +1.1 kcal/mol. The modification of the duplex containing 12 consecutive dA-T/ψT base pairs produces a ΔTm/mod of -0.9°C and a ΔΔG°50/mod of +1.2 kcal/mol[2].

Solvent & Solubility
In Vitro: 

DMSO : ≥ 61.17 mg/mL (252.53 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.1283 mL 20.6415 mL 41.2831 mL
5 mM 0.8257 mL 4.1283 mL 8.2566 mL
10 mM 0.4128 mL 2.0642 mL 4.1283 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Kinase Assay

Thermal DNA duplex denaturation studies are performed with templates containing up to twelve consecutive dA residues that are paired with its complement template containing consecutive T or Pseudothymidine (ψT) residues. Experiments are performed in a buffer (45 mM NaCl, 45 mM sodium citrate, pH 8.1, final vol. 1.5 mL) containing template and its complement (1.5 μM of each). Absorbance (260 nm) is monitored over a range of 25.0 to 90.0°C with a change in temperature of 0.5°C/min for five heating cycles. The initial heating cycle is discarded and the Tm is determined by averaging the temperatures of the remaining four cycles. The ΔTm between similar duplexes is calculated by subtracting the Tm of the duplex containing standard bases from the Tm of the duplex containing C-glycosides (including Pseudothymidine)[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

242.23

Formula

C₁₀H₁₄N₂O₅

CAS No.

65358-15-8

SMILES

O=C(N1)C([[email protected]@H]2O[[email protected]](CO)[[email protected]@H](O)C2)=CN(C)C1=O

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
Pseudothymidine
Cat. No.:
HY-101969
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Pseudothymidine

Cat. No.: HY-101969