ERCC1-XPF-IN-2
Based on 1 Customer Validation
ERCC1-XPF-IN-2 is a potent ERCC1-XPF endonuclease inhibitor with an IC50 value of 0.6 μM. ERCC1-XPF-IN-2 shows activity in nucleotide excision repair, cisplatin enhancement and γH2AX assays.
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- Reinheit: 98.54%
- CAS. Nr.: 1808986-37-9
- Formel: C15H13Cl2NO3
- Molecular Weight:326.17
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Speicherung:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
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Biologische Aktivität
ERCC1-XPF[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A-375 | IC50 |
15.6 μM
Compound: 13
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Inhibition of ERCC1-XPF in human A375 cells assessed as reduction in direct nucleotide excision repair of UV-damaged pEGFP plasmid incubated for 234 hrs by recombinant GFP reporter assay
Inhibition of ERCC1-XPF in human A375 cells assessed as reduction in direct nucleotide excision repair of UV-damaged pEGFP plasmid incubated for 234 hrs by recombinant GFP reporter assay
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[PMID: 26318993] |
ERCC1-XPF-IN-2 (compound 13) (0-100 μM) shows FEN-1 and DNase I activity with IC50s of >100, >100 μM, respectively[1].
ERCC1-XPF-IN-2 slow binding kinetics with an Kd value of ~30 μM[1].
ERCC1-XPF-IN-2 shows not toxic to Hep-G2 cells at 10 μM and relatively short mouse and human microsomal half-lives with t1/2 value of 23 min and 28 min for mouse and human, respectively.
ERCC1-XPF-IN-2 (0-60 μM; 24 h) shows inhibition of nucleotide excision repair (NER) with an IC50 value of 15.6 μM in A375 cells[1].
ERCC1-XPF-IN-2 (0-60 μM) increases the cisplatin activity with no toxicity[1].
ERCC1-XPF-IN-2 (10 μM; 6h) causes a delay in DNA repair by a right shift towards higher numbers of γH2AX foci per cell[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:A375 cells
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Concentration:0-60 µM
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Incubation Time:
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Result:Showed no toxicity and increased the cisplatin activity up to 1.5-fold (PF50).
Chemical Information
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CAS. Nr. 1808986-37-9
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Appearance Solid
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Molecular Weight 326.17
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Formel C15H13Cl2NO3
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Color White to off-white
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SMILES
OC1=C(O)C=C(CNC(CC2=C(Cl)C=C(Cl)C=C2)=O)C=C1
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Lösungsmittel & Löslichkeit
DMSO : ≥ 250 mg/mL (766.47 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Reinheit & Dokumentation
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Data Sheet (271 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.0659 mL | 15.3294 mL | 30.6589 mL | 76.6471 mL |
| 5 mM | 0.6132 mL | 3.0659 mL | 6.1318 mL | 15.3294 mL | |
| 10 mM | 0.3066 mL | 1.5329 mL | 3.0659 mL | 7.6647 mL | |
| 15 mM | 0.2044 mL | 1.0220 mL | 2.0439 mL | 5.1098 mL | |
| 20 mM | 0.1533 mL | 0.7665 mL | 1.5329 mL | 3.8324 mL | |
| 25 mM | 0.1226 mL | 0.6132 mL | 1.2264 mL | 3.0659 mL | |
| 30 mM | 0.1022 mL | 0.5110 mL | 1.0220 mL | 2.5549 mL | |
| 40 mM | 0.0766 mL | 0.3832 mL | 0.7665 mL | 1.9162 mL | |
| 50 mM | 0.0613 mL | 0.3066 mL | 0.6132 mL | 1.5329 mL | |
| 60 mM | 0.0511 mL | 0.2555 mL | 0.5110 mL | 1.2775 mL | |
| 80 mM | 0.0383 mL | 0.1916 mL | 0.3832 mL | 0.9581 mL | |
| 100 mM | 0.0307 mL | 0.1533 mL | 0.3066 mL | 0.7665 mL |
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