Art. -Nr.: HY-108903A

Hyaluronidase, Ovine testes

15 Cited Publications

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Based on 15 publication(s) in Google Scholar

Hyaluronidase, Ovine testes is an endoglycosidase. Hyaluronidase, Ovine testes specifically degrades Hyaluronic acid (HY-B0633A) and Chondroitin sulfate (HY-B2162) by hydrolyzing β-glycosidic bonds in acidic mucopolysaccharides. Hyaluronidase, Ovine testes disperses follicular cells during fertilization by breaking down the hyaluronic acid-rich cumulus. Hyaluronidase, Ovine testes can be used in the study of fertility-related diseases.

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Reinheit: 95.00%
CAS. Nr.: 37326-33-3
Molecular Weight:55000 (average)

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

Hyaluronidase, Ovine testes Chemical Structure

CAS. Nr.: 37326-33-3

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- In Vitro
- EC Number
- Specific Activity
- Unit Definition
Chemical Information
Publications (15)
Lösungsmittel & Löslichkeit
- In Vitro
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

In Vitro

Hyaluronidase inhibition test
Mix 10 μL of 8 mg/mL Hyaluronidase with the sample in a buffer solution (0.1 M acetate buffer, pH 3.6) and add it to a test tube. Incubate at 37 °C for 20 minutes.
(2) Add 20 μL of 12.5 mM calcium chloride to the mixture and incubate for another 20 minutes at 37°C.
(3) The activated Ca2+ mixture was treated with 50 μL of 2.4 μg/mL hyaluronic acid in the buffer and further incubated at 37°C for 40 minutes.
(4) Add 2 μL of 0.4 N sodium hydroxide and 20 μL of 0.4 N potassium tetraborate tetrahydrate, and then incubate in a water bath at 100°C for 3 minutes.
After cooling to room temperature, add 600 μL of DMAB solution to the mixture and incubate at 37°C for 20 minutes.
(6) Measure the absorbance at 585 nm using an microplate reader.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

EC Number

3.2.1.35

Specific Activity

≥400 U/mg protein

Unit Definition

One unit is defined as the amount of enzyme that can cause a change in A600 of 0.330 per minute at pH 5.35, 37°C in a 2.0 mL reaction mixture (45 min assay).

Chemical Information

CAS. Nr. 37326-33-3
Appearance Solid
Molecular Weight 55000 (average)
Color Off-white to yellow
SMILES

[Hyaluronidase, Ovine testes]
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Publications (15)

All IHCIn Vivo Efficacy Study

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Mol Cancer

Organoids derived from patients provide a new opportunity for research and individualized treatment of malignant peritoneal mesothelioma. [Abstract]

2024 Jan 10;23(1):12. PMID: 38200517
Cancer Res

Pancreatic Neuroendocrine Tumors Secrete Apolipoprotein E to Induce Tip Endothelial Cells That Remodel the Tumor-Stroma Ratio and Promote Cancer Progression. [Abstract]

2025 Aug 1;85(15):2805-2819. PMID: 40623046

Hyaluronidase, Ovine testes purchased from MedChemExpress. Usage Cited in: Cancer Res. 2025 Aug 1;85(15):2805-2819. [Abstract]

B, Growth curves of PDX mice treated with PBS, everolimus, PEGPH20 (Hyaluronidase), and everolimus combined with PEGPH20 (n = 5 per group). C, H&E staining and IHC were used to demonstrate the effects of different drug administration strategies on tumor stroma and CD31+ cell and αSMA⁺ cell number

Hyaluronidase, Ovine testes purchased from MedChemExpress. Usage Cited in: Cancer Res. 2025 Aug 1;85(15):2805-2819. [Abstract]

E and F, Flow cytometry analysis was used to demonstrate the effects of different drug administration strategies on the proportion of TipECs. Hyaluronidase (PEGPH20)

Hyaluronidase, Ovine testes purchased from MedChemExpress. Usage Cited in: Cancer Res. 2025 Aug 1;85(15):2805-2819. [Abstract]

The effects of different drug administration strategies in the BON1+ HUVEC (4:1) model. Hyaluronidase (PEGPH20)
Nat Commun

TRABID inhibition activates cGAS/STING-mediated anti-tumor immunity through mitosis and autophagy dysregulation. [Abstract]

2023 May 26;14(1):3050. PMID: 37237031
Theranostics

Platelets promote CRC by activating the C5a/C5aR1 axis via PSGL-1/JNK/STAT1 signaling in tumor-associated macrophages. [Abstract]

2023 Mar 27;13(6):2040-2056. PMID: 37064877
Adv Sci (Weinh)

Screening and Identification of Novel DNA Aptamer for Targeted Delivery to Injured Podocytes in Glomerular Diseases. [Abstract]

2025 May;12(20):e2412356. PMID: 40178289
J Control Release

Hydrogel-immobilized coacervate droplets enable microenvironment-responsive sustained drug release to accelerate diabetic foot ulcers healing. [Abstract]

2026 Jun 10:394:114858. PMID: 41881075
Mater Today Bio

Mannosylated graphene oxide nanotherapeutics co-delivering docetaxel and a STING agonist reprogram myeloid cells and potentiate antitumor immunity. [Abstract]

2026 Mar 31:38:103086. PMID: 42006712
Cell Biosci

Tumor-derived interleukin-1 receptor antagonist exhibits immunosuppressive functions and promotes pancreatic cancer. [Abstract]

2023 Aug 10;13(1):147. PMID: 37563620
J Mol Cell Biol

Microtubule-associated CCDC112 is essential for spermiogenesis and male fertility in mice. [Abstract]

2026 Jun 25:mjag019. PMID: 42348320
Int J Mol Sci

Melatonin Rescues Heat Stress-Induced Suppression of TCA Cycle and Mitochondrial Damage in Goat Sertoli Cells. [Abstract]

2025 Nov 27;26(23):11475. PMID: 41373630
Cell Oncol (Dordr)

GALNT6 drives lenvatinib resistance in hepatocellular carcinoma through autophagy and cancer-associated fibroblast activation. [Abstract]

2024 Dec;47(6):2439-2460. PMID: 39718738
Reproduction

Dynamic regulation of astral microtubule growth in the meiotic spindle of mouse oocyte. [Abstract]

2026 Jan 15;171(1):xaaf013. PMID: 41575147
Oral Dis

Thy1+ Periosteal Osteoprogenitors Contribute to Mandibular Bone Defect Healing in Mice. [Abstract]

2026 Apr 15. PMID: 41986910
Drug Dev Ind Pharm

Multiple stimulus-response berberine plus baicalin micelles with particle size-charge-release triple variable properties for breast cancer therapy. [Abstract]

2023 Feb;49(2):189-206. PMID: 36971392
Vet Med Int

Sericin Supplementation Prior to and During In Vitro Maturation Mitigates H2O2-Induced Oxidative Stress in Bovine Oocytes. [Abstract]

2026 May 21:2026:6119294. PMID: 42180161

Lösungsmittel & Löslichkeit

In Vitro:

H₂O : ≥ 100 mg/mL

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (270 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Krishnapillai A M, et al. Characterisation of Norway lobster (Nephrops norvegicus) hyaluronidase and comparison with sheep and bovine testicular hyaluronidase. Food chemistry, 1999, 65(4): 515-521.

[2]. SAKAI Y, et al. Immunohistochemical demonstration of hyaluronidase in the sheep testis. Acta Histochemica et Cytochemica, 1979, 12(2): 141-150.

[3]. Modarressi SM, et al. Enhancing hyaluronidase enzyme activity: Insights from advancement in bovine and ovine testicular hyaluronidase purification. J Chromatogr B Analyt Technol Biomed Life Sci. 2024 Feb 15;1234:124031. [Content Brief]

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

Hyaluronidase, Ovine testes

15 Cited Publications

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Hyaluronidase, Ovine testes

Publications Citing Use of MedChemExpress (MCE) Hyaluronidase, Ovine testes

Biologische Aktivität

Chemical Information

Publications (15)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Calculators

Help & FAQs

Hyaluronidase, Ovine testes Related Classifications

HY-108903A Related Classifications