Barbadin

Name: Barbadin
Brand: MedChemExpress
SKU: HY-119706
Rating: 5.0 (16 reviews)

Cat. No.: HY-119706 Purity: 98.93%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Barbadin is a novel and selective β-arrestin/β2-adaptin interaction inhibitor, has IC₅₀ values of 19.1 μM for β-arrestin1 and 15.6 μM for β-arrestin2. Barbadin blocks agonist-promoted endocytosis of the prototypical β2-adrenergic, V2-vasopressin and angiotensin-II type-1 receptors. Barbadin can induce apoptosis.

For research use only. We do not sell to patients.

CAS No. : 356568-70-2

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
2 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 16 publication(s) in Google Scholar

16 Publications Citing Use of MCE Barbadin

Bio/Physico-chemical Assay

Flow Cytometry

Cell Migration/Invasion Assay

: Barbadin purchased from MedChemExpress. Usage Cited in: Neuron. 2025 Nov 26:S0896-6273(25)00842-6. [Abstract]; Barbadin (100 μM; 2 h) was used to inhibit β-arrestin signaling and could indeed eliminate the inhibitory effect of MF-8 on LTP.

: Barbadin purchased from MedChemExpress. Usage Cited in: Drug Des Devel Ther. 2024 Mar 11:18:781-799.; Barbadin (50μM) inhibited β-arr2 expression compared with the blank control, together with the inhibition of SRECA2a and SUMO1 protein expression, and blocked the effect of QFYXF, which upregulated SERCA2a and SUMO1 expression in AngII-induced hypertrophic NRCMs.

: Barbadin purchased from MedChemExpress. Usage Cited in: Sci Signal. 2023 Apr 4;16(779):eabl4283. [Abstract]; Migration of sDCs toward 100 nM 7α,25-OHC upon preincubation with DMSO (vehicle) or Barbadin (50-100 μM; 30 min). Barbadin significantly decreased the migration of sDCs toward 7α,25-OHC in a concentration-dependent manner.

: Barbadin purchased from MedChemExpress. Usage Cited in: Sci Signal. 2023 Apr 4;16(779):eabl4283. [Abstract]; Analysis of 7α,25-OHC-induced signaling by GPR183 upon preincubation with either DMSO (vehicle) or Barbadin (50 μM; 1 h). Barbadin decreased ligand-induced β-arrestin1 and β-arrestin2 recruitment to GPR183 but not its G protein signaling.

: Barbadin purchased from MedChemExpress. Usage Cited in: Cell Chem Biol. 2022 Jan 20;29(1):67-73.e3. [Abstract]; The β-arrestin inhibitor Barbadin (100 μM; 30 min) prolongs PLD activity downstream of PTHR1–G_q signaling in hPTHR1-293 cells.

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Arrestin Isoform Specific Products:

View All Isoforms

Arrestin-2/β-Arrestin 1 Arrestin-3/β-Arrestin 2

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Arrestin-3/β-Arrestin 2

In Vitro

Barbadin (4 h) treatment reduces cell viability and induces apoptosis^[2].
Barbadin (2 h) treatment arrests breast cancer cells in G0/G1 phase^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[2]

Cell Line:	MDA MB-231 cells
Concentration:
Incubation Time:	4 hours
Result:	Exhibited morphological characteristics of apoptosis including shrinkage, rounding and detachment, the percent of cell viability was reduced to 69.1% and apoptosis was developed in 29.9% of cells starved with EBSS (Earle’s balanced salt solution).

Cell Cycle Analysis^[2]

Cell Line:	MDA MB-231 cells
Concentration:
Incubation Time:	2 hours
Result:	Arrested 63.7% of cells in G0/G1 phase.

Molecular Weight

333.41

Formula

C₁₉H₁₅N₃OS

CAS No.

356568-70-2

Appearance

Solid

Color

White to light yellow

SMILES

O=C1C(C(C2=CC=C(CC3=CC=CC=C3)C=C2)=CS4)=C4N=CN1N

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (149.97 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9993 mL	14.9966 mL	29.9931 mL
5 mM	0.5999 mL	2.9993 mL	5.9986 mL
10 mM	0.2999 mL	1.4997 mL	2.9993 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.50 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.50 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.93%

Select Batch:

Data Sheet (278 KB) SDS (251 KB)

COA (254 KB) HNMR (262 KB) LCMS (281 KB)

Handling Instructions (2659 KB)

References

[1]. Beautrait A, et al. A new inhibitor of the β-arrestin/AP2 endocytic complex reveals interplay between GPCR internalization and signalling. Nat Commun. 2017 Apr 18;8:15054. doi: 10.1038/ncomms15054. [Content Brief]

[2]. Thoria Donia, et al. β-Arrestin inhibition induces autophagy, apoptosis, G0/G1 cell cycle arrest in agonist-activated V2R receptor in breast cancer cells. Med Oncol. 2021 Mar 15;38(4):38. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.9993 mL	14.9966 mL	29.9931 mL	74.9828 mL
	5 mM	0.5999 mL	2.9993 mL	5.9986 mL	14.9966 mL
	10 mM	0.2999 mL	1.4997 mL	2.9993 mL	7.4983 mL
	15 mM	0.2000 mL	0.9998 mL	1.9995 mL	4.9989 mL
	20 mM	0.1500 mL	0.7498 mL	1.4997 mL	3.7491 mL
	25 mM	0.1200 mL	0.5999 mL	1.1997 mL	2.9993 mL
	30 mM	0.1000 mL	0.4999 mL	0.9998 mL	2.4994 mL
	40 mM	0.0750 mL	0.3749 mL	0.7498 mL	1.8746 mL
	50 mM	0.0600 mL	0.2999 mL	0.5999 mL	1.4997 mL
	60 mM	0.0500 mL	0.2499 mL	0.4999 mL	1.2497 mL
	80 mM	0.0375 mL	0.1875 mL	0.3749 mL	0.9373 mL
	100 mM	0.0300 mL	0.1500 mL	0.2999 mL	0.7498 mL

Barbadin Related Classifications

Others

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Barbadin356568-70-2ArrestinApoptosisendocytosisV2-vasopressinangiotensin-II type-1MDA MB-231 cell,Inhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Requested Quantity *

Applicant Name *

Salutation

Email Address *

Phone Number *

Department

Organization Name *

City

State

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

Barbadin

Customer Review

Barbadin Related Antibodies

16 Publications Citing Use of MCE Barbadin

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Arrestin Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Barbadin Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Barbadin Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report