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Art. -Nr.: HY-117612

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KS-99

KS-99 is a dual inhibitor with both tubulin polymerization inhibitory activity and Akt pathway inhibitory activity. KS-99 inhibits cancer cell proliferation and induces cancer cell apoptosis. KS-99 can be used in research related to colorectal cancer, breast cancer, lung epithelial cancer and melanoma.

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CAS. Nr.: 1344698-28-7
Formel: C₁₇H₁₀Br₂N₂O₂S
Molecular Weight:466.15

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

KS-99 Chemical Structure

CAS. Nr.: 1344698-28-7

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Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
Chemical Information
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Alle Akt Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	2.53 μM Compound: 11	Cytotoxicity against human A549 cells after 48 hrs by MTS assay Cytotoxicity against human A549 cells after 48 hrs by MTS assay	[PMID: 21920762]
HT-29	IC₅₀	1.14 μM Compound: 11	Cytotoxicity against human HT-29 cells after 48 hrs by MTS assay Cytotoxicity against human HT-29 cells after 48 hrs by MTS assay	[PMID: 21920762]
MCF7	IC₅₀	24.7 μM Compound: 11	Cytotoxicity against human MCF7 cells after 48 hrs by MTS assay Cytotoxicity against human MCF7 cells after 48 hrs by MTS assay	[PMID: 21920762]
MEF	IC₅₀	>20 μM Compound: 11	Cytotoxicity against mouse MEF cells after 48 hrs by MTS assay Cytotoxicity against mouse MEF cells after 48 hrs by MTS assay	[PMID: 21920762]
RPMI-8226	IC₅₀	0.5 μM Compound: 12; KS99	Cytotoxicity against human RPMI-8226 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay Cytotoxicity against human RPMI-8226 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay	[PMID: 35247756]
U-266	IC₅₀	0.5 μM Compound: 12; KS99	Cytotoxicity against human U-266 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay Cytotoxicity against human U-266 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay	[PMID: 35247756]
UACC-903	IC₅₀	2.23 μM Compound: 11	Cytotoxicity against human UACC903 cells after 48 hrs by MTS assay Cytotoxicity against human UACC903 cells after 48 hrs by MTS assay	[PMID: 21920762]

In Vitro

KS-99 (Compound 11) (0.5-50 μM; 48 h) potently inhibits the viability of HT29, A549 and UACC903 cancer cells, with IC₅₀ values of 1.14 μM, 2.53 μM and 2.23 μM, respectively; it shows significantly lower activity against MCF-7 cells and exhibits low toxicity toward normal MEF cells after 48 h of treatment^[1].
KS-99 (1.25-5.0 μM; 24 h) induces dose-dependent apoptosis in human colon cancer cell line HT29, with 66.98% of cells undergoing apoptosis after treatment with 5.0 μM for 24 h^[1].
KS-99 (1.25-5.0 μM; 24 h) dose-dependently activates caspase-3/7 in human colon cancer cell line HT29, confirming that it induces the apoptotic pathway^[1].
KS-99 (5 μM; 48 h) induces DNA fragmentation in human colon cancer cell line HT29^[1].
KS-99 (1-2 μM; 24 h) downregulates the total Akt protein level in human HT29 colon cancer cells, and completely abolishes Akt phosphorylation following 24 h of treatment at concentrations of 1 μM and 2 μM, acting as a potent inhibitor of the Akt pathway^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[1]

Cell Line:

human colorectal cancer HT29 cells, human breast cancer MCF-7 cells, human lung epithelial carcinoma A549 cells, human melanoma UACC903 cells, mouse embryonic fibroblasts (MEF) cells
Concentration:

0.5-50 μM
Incubation Time:

48 h
Result:

Exhibited an IC₅₀ of 1.14 μM against HT29 cells, 24.70 μM against MCF-7 cells, 2.53 μM against A549 cells, 2.23 μM against UACC903 cells, and an IC₅₀ > 20 μM against MEF cells.

Apoptosis Analysis^[1]

Cell Line:

human colorectal cancer HT29 cells
Concentration:

1.25 μM, 2.5 μM, 5.0 μM
Incubation Time:

24 h
Result:

Induced apoptosis in 11.47% of HT29 cells at 1.25 μM, 39.46% at 2.5 μM, and 66.98% at 5.0 μM.

Apoptosis Analysis^[1]

Cell Line:

human colorectal cancer HT29 cells
Concentration:

5 μM
Incubation Time:

48 h
Result:

Resulted in a significant number of TUNEL-positive HT29 cells, indicating DNA fragmentation as a hallmark of apoptosis.

Western Blot Analysis^[1]

Cell Line:

human colorectal cancer HT29 cells
Concentration:

1 μM, 2 μM
Incubation Time:

24 h
Result:

Reduced Akt protein levels and strongly suppressed the phosphorylation of Akt, eliminating detectable phosphorylated Akt at 1 μM and 2 μM.

Chemical Information

CAS. Nr. 1344698-28-7
Molecular Weight 466.15
Formel C₁₇H₁₀Br₂N₂O₂S
SMILES

N#CSCC1=CC=C(C=C1)CN2C(=O)C(=O)C=3C=C(Br)C=C(Br)C32
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Reinheit & Dokumentation

Handling Instructions (2659 KB)

Verweise

[1]. Krishnegowda G, et al. Synthesis and biological evaluation of a novel class of isatin analogs as dual inhibitors of tubulin polymerization and Akt pathway. Bioorg Med Chem. 2011;19(20):6006-6014. [Content Brief]

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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Biologische Aktivität
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KS-99

Alle Akt Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Reinheit & Dokumentation

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KS-99 Related Classifications

HY-117612 Related Classifications