4-Methylumbelliferyl phosphate
Based on 1 publication(s) in Google Scholar
4-Methylumbelliferyl phosphate (4-MUP), an anionic organophosphate, is a acid and alkaline phosphatase fluorogenic substrate. 4-Methylumbelliferyl phosphate is also a nerve agent simulant.
For research use only. We do not sell to patients.
- Purity: 99.84%
- CAS No.: 3368-04-5
- Formula: C10H9O6P
- Molecular Weight:256.15
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) 4-Methylumbelliferyl phosphate
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Biological Activity
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
(1) Substrate Preparation: Prepare 4-MUP at a 0.6 mM to 1 mg/mL concentration in a buffer with an alkaline pH (e.g., Tris-NaCl pH 10, or Diethanolamine buffer).
(2) Sample Preparation: Use samples (e.g., lysates, supernatant) diluted in appropriate buffers.
(3) Incubation: Mix 1 part sample with 1-2 parts 4-MUP substrate solution.
(4) Reaction Conditions: Incubate at 37°C or room temperature for 30–60 minutes, protected from light.
(5) Detection: Measure fluorescence at excitation ~ 360 nm and emission ~ 440 nm.
(6) Stopping the Reaction: The reaction can be stopped by adding high pH buffers, such as 0.2 M Glycine/NaOH.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 3368-04-5
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Appearance Solid
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Molecular Weight 256.15
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Formula C10H9O6P
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Color White to off-white
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SMILES
O=C1C=C(C)C2=CC=C(OP(O)(O)=O)C=C2O1
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Synonyms
4-MUP; MUP
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (1)
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Journal Impact Factor
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Most Recent
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Research (Wash D C)
A Noninvasive Menstrual Blood-Based Diagnostic Platform for Endometriosis Using Digital Droplet Enzyme-Linked Immunosorbent Assay and Single-Cell RNA Sequencing. [Abstract]2025 Apr 1:8:0652. PMID: 40171018
Solvent & Solubility
H2O : 17.5 mg/mL (68.32 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Chambers JP, et al. Determination of serum acid phosphatase in Gaucher's disease using 4-methylumbelliferyl phosphate. Clin Chim Acta. 1977 Oct 1;80(1):67-77. [Content Brief]
[2]. Watanabe F, et al. The analysis of alkaline phosphatase isoenzyme using 4-methylumbelliferyl phosphate as substrate on a cellulose acetate membrane. Clin Chim Acta. 1979 Feb 1;91(3):273-6. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 3.9040 mL | 19.5198 mL | 39.0396 mL | 97.5991 mL |
| 5 mM | 0.7808 mL | 3.9040 mL | 7.8079 mL | 19.5198 mL | |
| 10 mM | 0.3904 mL | 1.9520 mL | 3.9040 mL | 9.7599 mL | |
| 15 mM | 0.2603 mL | 1.3013 mL | 2.6026 mL | 6.5066 mL | |
| 20 mM | 0.1952 mL | 0.9760 mL | 1.9520 mL | 4.8800 mL | |
| 25 mM | 0.1562 mL | 0.7808 mL | 1.5616 mL | 3.9040 mL | |
| 30 mM | 0.1301 mL | 0.6507 mL | 1.3013 mL | 3.2533 mL | |
| 40 mM | 0.0976 mL | 0.4880 mL | 0.9760 mL | 2.4400 mL | |
| 50 mM | 0.0781 mL | 0.3904 mL | 0.7808 mL | 1.9520 mL | |
| 60 mM | 0.0651 mL | 0.3253 mL | 0.6507 mL | 1.6267 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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