CIGB-552
Based on 1 Customer Validation
CIGB-552 is a cell-penetrating peptide with anti-tumor properties with the IC50 of 23 μM in H460 cells. CIGB-552 can increase the level of protein COMMD1. CIGB-552 significantly inhibits the NF-κB signaling pathway. CIGB-552 can promote apoptosis of the tumor cells. CIGB-552 can induce the accumulation of reactive oxygen species (ROS) in tumor cells. CIGB-552 has anti-inflammatory and anti-angiogenic effects. CIGB-552 can be used for the research of the lung cancer and colon cancer.
For research use only. We do not sell to patients.
- Purity: 98.66%
- CAS No.: 1630763-23-3
- Formula: C131H198N38O24
- Molecular Weight:2689.21
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
CIGB-552 (20-60 μM, 5 h) can increase the protein content of the anti-tumor related protein COMMD1[1].
CIGB-552 (25 μM, 0-12 h) promotes the ubiquitination degradation of RelA and inhibits NF-κB signaling in H460 cells[1].
CIGB-552 (25 μM, 0-24 h) can increase the level of pro apoptotic proteins and reduce the level of anti apoptotic proteins in H460 cells[1].
CIGB-552 (25 μM, 24-48 h) can induce apoptosis in lung cancer cells[1].
CIGB-552 (25 μM, 8 h) reduces cellular antioxidant capacity, leading to protein and lipid oxidative damage in H460 cells [1].
CIGB-552 (37.5 μM, 1 h) induces the accumulation of reactive oxygen species (ROS) by inhibiting SOD1 activity, selectively killing tumor cells[2].
CIGB-552 (75-150 μM, 24 h) can significantly inhibit TNF-α-induced NF-κB activation[3].
CIGB-552 (2.5-25 μM, 24 h) exerts anti angiogenic effects by inhibiting hypoxia induced HIF-1 activation through COMMD1[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:H460, H-125, H-82, LS174T, MDA-231 and PBMC cells
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Concentration:0-200 μM
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Incubation Time:48 h
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Result:Had better cytotoxicity against H460 (IC50 = 23 μM, H-125 (IC50 = 42 μM), H-82 (IC50 = 15 μM), LS174T (IC50 = 22 μM), MDA-231 (IC50 = 40 μM) and PBMC (IC50 = 249 μM) cells compared to the control group.
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Cell Line:H460, MCF7, and HT29 cells
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Concentration:20-60 μM, MG132 (HY-13259) as a control group
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Incubation Time:5 h
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Result:Increased the protein level of COMMD1 in three types of cancer cells.
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Cell Line:H460 cells
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Concentration:25 μM
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Incubation Time:24 h
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Result:Increased the level of protein Bax, decreased the amount of protein Bcl-2, and activated Caspase-3 and PARP cleavage.
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Cell Line:H460 cells
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Concentration:25 μM
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Incubation Time:24, 48 h
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Result:Significantly increased the apoptosis rate of cells compared to the control group.
| Species | Dose | Route | Tmax | AUC0-∞ | Vd/F | MRT | T1/2 | Cmax |
|---|---|---|---|---|---|---|---|---|
| Mice[4] | 5 ug | s.c. | 0.33 h | 161.30 ng·h/mL | 362.29 mL | 7 h | 8.10 h | 82.2 ng/mL |
CIGB-552 (0.2-1.4 mg/kg; s.c.; two times a week; for two weeks) significantly inhibits tumor growth in mice with colon cancer and has anti angiogenic effects[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:5×10<4/sup> TC-1 cells injected the C57/BL6 female mice (8 weeks, 18-20g)[2]
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Dosage:1 mg/kg, combination with 0.4 mg/kg Cisplatin (HY-17394)
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Administration:Subcutaneous injection (s.c.); three times a week for three weeks
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Result:Significantly reduced tumor volume of the cancer mice.
Improved the survival rate of the cancer mice.
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Animal Model:7×104 CT-26 cells injected the BALB/c mice[4]
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Dosage:0.2 or 0.7 mg/kg
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Administration:Subcutaneous injection (s.c.); two times a week for two weeks
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Result:Inhibited the tumor volume of tumor mice and caused apoptosis of cells at the tumor site.
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Animal Model:1×107 HT-29 cells injected the female athymic nu/nu(nude) mice[4]
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Dosage:0.7 or 1.4 mg/kg
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Administration:Subcutaneous injection (s.c.); two times a week for two weeks
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Result:Inhibited the tumor volume of tumor mice and reduced tumor microvascular density.
Chemical Information
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CAS No. 1630763-23-3
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Appearance Solid
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Molecular Weight 2689.21
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Formula C131H198N38O24
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Color White to off-white
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Sequence
Ac-His-Ala-Arg-Ile-Lys-{d-Pro}-Thr-Phe-Arg-Arg-{d-Leu}-Lys-Trp-Lys-Tyr-Lys-Gly-Lys-Phe-Trp
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Sequence Shortening
Ac-HARIK-{d-Pro}-TFRR-{d-Leu}-KWKYKGKFW
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
H2O : 17.3 mg/mL (6.43 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (284 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Fernández Massó JR, et al. The Antitumor Peptide CIGB-552 Increases COMMD1 and Inhibits Growth of Human Lung Cancer Cells. J Amino Acids. 2013;2013:251398. [Content Brief]
[2]. Gomez Rodriguez Y, et al. Synergic effect of anticancer peptide CIGB-552 and Cisplatin in lung cancer models. Mol Biol Rep. 2022 Apr;49(4):3197-3212. [Content Brief]
[3]. Daghero H, et al. The Anticancer Peptide CIGB-552 Exerts Anti-Inflammatory and Anti-Angiogenic Effects through COMMD1. Molecules. 2020 Dec 31;26(1):152. [Content Brief]
[4]. Vallespí MG, et al. Antitumor efficacy, pharmacokinetic and biodistribution studies of the anticancer peptide CIGB-552 in mouse models. J Pept Sci. 2014 Nov;20(11):850-9. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 0.3719 mL | 1.8593 mL | 3.7186 mL | 9.2964 mL |
| 5 mM | 0.0744 mL | 0.3719 mL | 0.7437 mL | 1.8593 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
CIGB-552 Related Classifications
HY-P11115 Related Classifications
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