Crotonyl-CoA tetralithium 

Crotonyl-CoA tetralithium, a high-energy acyl donor, is an intermediate in the fermentation of butyric acid, and in the metabolism of lysine and tryptophan. Crotonyl-CoA tetralithium is important in the metabolism of fatty acids and amino acids. Crotonyl-CoA tetralithium acts as a substrate for p300’s histone crotonyltransferase activity, competing with acetyl-CoA for p300-mediated histone acylation reactions. Crotonyl-CoA tetralithium regulates global and gene-specific histone crotonylation levels in cells, with cellular concentration changes altering histone crotonylation at regulatory elements of activated genes. Crotonyl-CoA tetralithium serves as the substrate for crotonyl-CoA reductase/carboxylase (CCRC)-catalyzed NADPH-mediated reduction and carbon dioxide trapping to form unusual alkylmalonyl-CoA polyketide synthase extender units. Crotonyl-CoA tetralithium can be used for the research of LPS-induced inflammatory response^[1][2].

Crotonyl-CoA tetralithium directly stimulates transcription in a cell-free system more potently than acetylation, with a 1.66-fold greater transcript production^[1].
Crotonyl-CoA tetralithium, when cellular concentrations are increased via 2.5 mM, 5 mM, 10 mM sodium crotonate treatment (12 h), leads to dose-dependent increases in global H3K18Cr levels in HeLa S3 cells^[1].
Crotonyl-CoA tetralithium, when cellular concentrations are increased via 0 mM, 2.5 mM, 5 mM, 10 mM sodium crotonate pretreatment (6 h prior to 2 h LPS stimulation), enhanced H3K18Cr at de novo-activated gene promoters and increased their expression upon LPS stimulation in RAW 264.7 cells^[1].
Crotonyl-CoA tetralithium, when its cellular levels are reduced via ACSS2 knockdown (72 h prior to 2 h LPS stimulation), reduced H3K18Cr at de novo-activated gene promoters and attenuated their expression upon LPS stimulation in RAW 264.7 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

863.37

C₂₅H₄₀Li₄N₇O₁₇P₃S

116912-55-1

O[C@H]1[C@@H](O[C@@H]([C@H]1OP(O)(O)=O)COP(OP(OCC(C)(C)[C@@H](O)C(NCCC(NCCSC(/C=C/C)=O)=O)=O)(O)=O)(O)=O)N2C3=NC=NC(N)=C3N=C2.[4Li]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Sabari BR, et al. Intracellular crotonyl-CoA stimulates transcription through p300-catalyzed histone crotonylation. Mol Cell. 2015;58(2):203-215.  [Content Brief]

  [2]. Ray L, et al. A crotonyl-CoA reductase-carboxylase independent pathway for assembly of unusual alkylmalonyl-CoA polyketide synthase extender units. Nat Commun. 2016;7:13609. Published 2016 Dec 21.  [Content Brief]