(Rac)-Brassinazole
(Rac)-Brassinazole, triazole-type compound, is a brassinosteroid (BR) biosynthesis inhibitor. (Rac)-Brassinazole increases inhibition of CYP90B in BR biosynthesis
For research use only. We do not sell to patients.
- CAS No.: 224047-41-0
- Formula: C18H18ClN3O
- Molecular Weight:327.81
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
(Rac)-Brassinazole (compound 5) does not retard rice stem elongation, and retardation is recovered by the addition of gibberellin (GA3), suggesting that such retardation is due to the inhibition of gibberellin biosynthesis[1].
(Rac)-Brassinazol (compound 31) results in a reduction in the effect on AtKO in gibberellin (GA) biosynthesis[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 224047-41-0
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Appearance Solid
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Molecular Weight 327.81
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Formula C18H18ClN3O
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SMILES
OC(C1=CC=CC=C1)(C)C(CC2=CC=C(Cl)C=C2)N3N=CN=C3
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 50 mg/mL (152.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (7.63 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Y K Min, et al. New Lead Compounds for Brassinosteroid Biosynthesis Inhibitors. Bioorg Med Chem Lett. 1999 Feb 8;9(3):425-30. [Content Brief]
[2]. Eriko Sasaki, et al. Uniconazole, a Cytochrome P450 Inhibitor, Inhibits Trans-Zeatin Biosynthesis in Arabidopsis. Phytochemistry. 2013 Mar;87:30-8. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.0505 mL | 15.2527 mL | 30.5055 mL | 76.2637 mL |
| 5 mM | 0.6101 mL | 3.0505 mL | 6.1011 mL | 15.2527 mL | |
| 10 mM | 0.3051 mL | 1.5253 mL | 3.0505 mL | 7.6264 mL | |
| 15 mM | 0.2034 mL | 1.0168 mL | 2.0337 mL | 5.0842 mL | |
| 20 mM | 0.1525 mL | 0.7626 mL | 1.5253 mL | 3.8132 mL | |
| 25 mM | 0.1220 mL | 0.6101 mL | 1.2202 mL | 3.0505 mL | |
| 30 mM | 0.1017 mL | 0.5084 mL | 1.0168 mL | 2.5421 mL | |
| 40 mM | 0.0763 mL | 0.3813 mL | 0.7626 mL | 1.9066 mL | |
| 50 mM | 0.0610 mL | 0.3051 mL | 0.6101 mL | 1.5253 mL | |
| 60 mM | 0.0508 mL | 0.2542 mL | 0.5084 mL | 1.2711 mL | |
| 80 mM | 0.0381 mL | 0.1907 mL | 0.3813 mL | 0.9533 mL | |
| 100 mM | 0.0305 mL | 0.1525 mL | 0.3051 mL | 0.7626 mL |
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