Itaconate-alkyne
Based on 5 publication(s) in Google Scholar
Itaconate-alkyne (ITalk) is a specific bioorthogonal probe for quantitative and site-specific chemoproteomic profiling of Itaconation in living cells. Itaconate-alkyne, a functional analogue of Itaconate, exhibits comparable antiinflammatory effect with Itaconate and enables the labeling of bona fide targets of Itaconate. Itaconate-alkyne is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
For research use only. We do not sell to patients.
- Purity: 98.01%
- CAS No.: 2454181-83-8
- Formula: C13H18O4
- Molecular Weight:238.28
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Itaconate-alkyne
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Customer Validation & Images
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IP
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IP
Biological Activity
Itaconate-alkyne (ITalk; 0.005, 0.01, 0.05, 0.1, 0.5, 1, 5, 10 mM; 0-4 hours) has the concentration- and time-dependent labeling in the lysates of Raw264.7 cells[1].
Itaconate-alkyne (100 μM; for 4 hours) significantly increases the protein level of NRF-2 in LPS (100 ng/mL)-stimulated Raw264.7 cells and significantly inhibits lactate production in LPS-stimulated Raw264.7 cells. Itaconate-alkyne significantly inhibits the secretion of interlukin-1β (IL-1β) in LPS-timulated Raw264.7 cells[1].
Itaconate-alkyne (100 μM; for 12 hours) does not affect the cell viability of Raw264.7 cells[1].
Itaconate-alkyne can capture itaconate targets including DNA damage-binding protein 1 (DDB1), Gelsolin (GSN), ATP-citrate synthase (ACLY) and Adenylate kinase 2 (AK2) in inflammatory macrophages[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2454181-83-8
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Appearance Solid
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Molecular Weight 238.28
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Formula C13H18O4
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Color White to off-white
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SMILES
O=C(O)C(CC(OCCCCCCC#C)=O)=C
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Synonyms
ITalk
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (5)
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Journal Impact Factor
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Most Recent
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Cell Rep
4-octyl itaconate inhibits cytokine-mediated inflammation via alkylation of TYK2 and JAK1. [Abstract]2026 Mar 30;45(4):117179. PMID: 41915469 -
Cell Rep
IRG1 catalyzed energy metabolite itaconic acid restrains type I interferon-dependent immune responses by alkylation of TBK1. [Abstract]2025 Sep 26;44(10):116336. PMID: 41014555
Itaconate-alkyne purchased from MedChemExpress. Usage Cited in: Cell Rep. 2025 Sep 26;44(10):116336. [Abstract]
Immunoblot analysis of the alkylation of TBK1 by selective labeling with Itaconate-alkyne (ITalk) in PMs or TBK1-MYC-overexpressing HEK293T cells treated with ITA.
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Int Immunopharmacol
Neutrophil aconitate decarboxylase 1/itaconate axis suppresses extracellular traps via alkylating GSDMD and alleviates murine liver injury. [Abstract]2026 Jun 1:178:116578. PMID: 41915992 -
STAR Protoc
Click-chemistry-based protocol for detecting 4-octyl-itaconate-alkylated proteins in primary mouse macrophages. [Abstract]2024 Sep 18;5(4):103314. PMID: 39298323
Itaconate-alkyne purchased from MedChemExpress. Usage Cited in: STAR Protoc. 2024 Sep 18;5(4):103314. [Abstract]
Immunoblot analysis of proteins in streptavidin pull-down from clicked lysates of BMDMs treated with Itaconate-alkyne (ITalk) or DMSO for 12 h. Streptavidin-HRP is used to probe the labeling protein. STING is an example of the probe-modified protein.
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Solvent & Solubility
DMSO : 250 mg/mL (1049.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (8.73 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (8.73 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (269 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 4.1967 mL | 20.9837 mL | 41.9674 mL | 104.9186 mL |
| 5 mM | 0.8393 mL | 4.1967 mL | 8.3935 mL | 20.9837 mL | |
| 10 mM | 0.4197 mL | 2.0984 mL | 4.1967 mL | 10.4919 mL | |
| 15 mM | 0.2798 mL | 1.3989 mL | 2.7978 mL | 6.9946 mL | |
| 20 mM | 0.2098 mL | 1.0492 mL | 2.0984 mL | 5.2459 mL | |
| 25 mM | 0.1679 mL | 0.8393 mL | 1.6787 mL | 4.1967 mL | |
| 30 mM | 0.1399 mL | 0.6995 mL | 1.3989 mL | 3.4973 mL | |
| 40 mM | 0.1049 mL | 0.5246 mL | 1.0492 mL | 2.6230 mL | |
| 50 mM | 0.0839 mL | 0.4197 mL | 0.8393 mL | 2.0984 mL | |
| 60 mM | 0.0699 mL | 0.3497 mL | 0.6995 mL | 1.7486 mL | |
| 80 mM | 0.0525 mL | 0.2623 mL | 0.5246 mL | 1.3115 mL | |
| 100 mM | 0.0420 mL | 0.2098 mL | 0.4197 mL | 1.0492 mL |
Itaconate-alkyne Related Classifications
HY-133870 Related Classifications
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