EG01377 dihydrochloride
Based on 5 publication(s) in Google Scholar
EG01377 dihydrochloride is a potent, bioavailable and selective inhibitor of neuropilin-1 (NRP1), with a Kd of 1.32 μM, and IC50s of 609 nM for both NRP1-a1 and NRP1-b1. EG01377 dihydrochloride has antiangiogenic, antimigratory, and antitumor effects.
For research use only. We do not sell to patients.
- Purity: 98.98%
- CAS No.: 2749438-61-5
- Formula: C26H32Cl2N6O6S2
- Molecular Weight:659.60
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Storage:
-20°C, stored under nitrogen, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) EG01377 dihydrochloride
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Customer Validation & Images
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WB
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Cell Proliferation/Viability Assay
Biological Activity
IC50: 609 nM (NRP1-a1 and NRP1-b)[1]
EG01377 (3-30 μM; 30 minutes) inhibits vascular endothelial growth factor A (VEGF-A) stimulated tyrosine phosphorylation of VEGF-R2/KDR[1].
EG01377 (30 μM) is able to significantly reduce HUVEC cell migration in response to VEGFA[1].
EG01377 (30 μM; 5 days) can delay the VEGF-induced wound closure[1].
EG01377 (30 μM) reduces network area, length, and branching points[1].
EG01377 (30 μM; 7 days) reduces VEGF-induced angiogenesis[1].
EG01377 (30 μM; 7 days) in combination with VEGFA reduces A375P (malignant melanoma) spheroid outgrowth[1].
EG01377 (500 nM; 2 hours) blocks the production of transforming growth factor beta (TGFβ) by Nrp1+ regulatory T-cell SMAD3/AKT (Tregs) in the presence of tumor cell-derived factors[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Human umbilical vein endothelial cells (HUVECs)
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Concentration:3, 10, 30 μM
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Incubation Time:30 minutes
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Result:Inhibited VEGF-A stimulated tyrosine phosphorylation of VEGF-R2/KDR with an IC50 of 30 μM.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:6-8 week-old BABL/c female mice[1]
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Dosage:2 mg/kg (Pharmacokinetic Analysis)
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Administration:I.v. administration
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Result:The half time (T1/2) of 4.29 h.
Chemical Information
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CAS No. 2749438-61-5
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Appearance Solid
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Molecular Weight 659.60
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Formula C26H32Cl2N6O6S2
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Color White to off-white
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SMILES
O=S(C1=CC(C2=CC=C(CN)C=C2)=CC3=C1OCC3)(NC4=C(C(N[C@@H](CCC/N=C(N)/N)C(O)=O)=O)SC=C4)=O.Cl.Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, stored under nitrogen, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
Publications (5)
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Journal Impact Factor
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Most Recent
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Nat Cancer
MEF2D-expressing cancer precursors reprogram tissue-resident macrophages to support liver tumorigenesis. [Abstract]2025 Dec;6(12):1955-1975. PMID: 41131393 -
Cell Death Dis
NRP1 promotes prostate cancer progression via modulating EGFR-dependent AKT pathway activation. [Abstract]2023 Feb 25;14(2):159. PMID: 36841806
EG01377 dihydrochloride purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2023 Feb 25;14(2):159. [Abstract]
EG01377 (20, 40, 80 µM) attenuates the phosphorylation of EGFR, AKT, GSK3β, and mTOR in PC-3 and DU-145 cells.
EG01377 dihydrochloride purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2023 Feb 25;14(2):159. [Abstract]
EG01377 (20, 40, 80 µM; 14 days) significantly attenuates clone formation ability of PC-3 and DU-145 cells in a concentration-dependent manner.
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Proc Natl Acad Sci U S A
Targeting the ANGPTL4/NRP1/ABL1/RAD51 axis reverses cisplatin resistance by impairing DNA damage repair in head and neck cancer. [Abstract]2026 Mar 31;123(13):e2510265123. PMID: 41886374 -
Cancers (Basel)
Immune Checkpoint Neuropilins as Novel Biomarkers and Therapeutic Targets for Pancreatic Cancer. [Abstract]2023 Apr 10;15(8):2225. PMID: 37190154 -
J Virol
Differential expression of viral entry protein neuropilin 1 (NRP1) and neuropilin 2 (NRP2) in fatal COVID-19. [Abstract]2025 Oct 29:e0138425. PMID: 41159753
Solvent & Solubility
DMSO : 200 mg/mL (303.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (7.58 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (7.58 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (282 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.5161 mL | 7.5804 mL | 15.1607 mL | 37.9018 mL |
| 5 mM | 0.3032 mL | 1.5161 mL | 3.0321 mL | 7.5804 mL | |
| 10 mM | 0.1516 mL | 0.7580 mL | 1.5161 mL | 3.7902 mL | |
| 15 mM | 0.1011 mL | 0.5054 mL | 1.0107 mL | 2.5268 mL | |
| 20 mM | 0.0758 mL | 0.3790 mL | 0.7580 mL | 1.8951 mL | |
| 25 mM | 0.0606 mL | 0.3032 mL | 0.6064 mL | 1.5161 mL | |
| 30 mM | 0.0505 mL | 0.2527 mL | 0.5054 mL | 1.2634 mL | |
| 40 mM | 0.0379 mL | 0.1895 mL | 0.3790 mL | 0.9475 mL | |
| 50 mM | 0.0303 mL | 0.1516 mL | 0.3032 mL | 0.7580 mL | |
| 60 mM | 0.0253 mL | 0.1263 mL | 0.2527 mL | 0.6317 mL | |
| 80 mM | 0.0190 mL | 0.0948 mL | 0.1895 mL | 0.4738 mL | |
| 100 mM | 0.0152 mL | 0.0758 mL | 0.1516 mL | 0.3790 mL |
EG01377 dihydrochloride Related Classifications
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