Tenovin-3
Based on 1 Customer Validation
Tenovin-3 is a p53 activator.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 1011301-27-1
- Formula: C18H21N3OS
- Molecular Weight:327.44
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Apoptosis Compound Library
HY-L003
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Cell Cycle/DNA Damage Compound Library
HY-L004
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Epigenetics Compound Library
HY-L005
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Histone Modification Research Compound Library
HY-L024
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Anti-Cancer Compound Library
HY-L025
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Autophagy Compound Library
HY-L029
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Anti-Aging Compound Library
HY-L034
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Antioxidant Compound Library
HY-L037
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Oxygen Sensing Compound Library
HY-L045
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Ferroptosis Compound Library
HY-L051
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Pyroptosis Compound Library
HY-L059
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Glutamine Metabolism Compound Library
HY-L064
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Anti-Pancreatic Cancer Compound Library
HY-L077
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Anti-Blood Cancer Compound Library
HY-L079
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Anti-Cancer Metabolism Compound Library
HY-L083
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Transcription Factor-Targeted Library
HY-L090
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Anti-Colorectal Cancer Compound Library
HY-L103
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Target Protein Ligand Library
HY-L129
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Metabolic Enzyme Compound Library
HY-L146
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Anti-Hematopathy Compound Library
HY-L171
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Anti-Ovarian Cancer Compound Library
HY-L173
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Multi-Target Compound Library
HY-L176
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Radioprotector Library
HY-L178
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Bioactive Compound Library Max
HY-L181
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Bioactive Compound Library
HY-L001
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Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library
HY-L199
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Cell Proliferation Compound Library
HY-L201
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High-Throughput Bioactive Compound Library
HY-L205
Biological Activity
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SIRT3 |
EV-A71 3D polymerase |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEK-293T | CC50 |
72.26 μM
Compound: 11
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Cytotoxicity against human HEK293T cells assessed as reduction in cell viability incubated for 48 hrs by CCK-8 assay
Cytotoxicity against human HEK293T cells assessed as reduction in cell viability incubated for 48 hrs by CCK-8 assay
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[PMID: 38775356] |
| HEK-293T | EC50 |
>10 μM
Compound: 11
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Anti-influenza activity against Influenza A virus H3N2 infected in HEK293T cells assessed as reduction in virus-induced cytopathic effect incubated for 48 hrs by CCK-8 assay
Anti-influenza activity against Influenza A virus H3N2 infected in HEK293T cells assessed as reduction in virus-induced cytopathic effect incubated for 48 hrs by CCK-8 assay
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[PMID: 38775356] |
| HEK-293T | EC50 |
0.151 μM
Compound: 11
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Anti-influenza activity against Influenza B virus Yamagata/16/88 infected in HEK293T cells assessed as reduction in virus-induced cytopathic effect incubated for 48 hrs by CCK-8 assay
Anti-influenza activity against Influenza B virus Yamagata/16/88 infected in HEK293T cells assessed as reduction in virus-induced cytopathic effect incubated for 48 hrs by CCK-8 assay
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[PMID: 38775356] |
| HEK-293T | EC50 |
0.167 μM
Compound: 11
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Anti-influenza activity against Influenza B virus Victoria/2/87 infected in HEK293T cells assessed as reduction in virus-induced cytopathic effect incubated for 48 hrs by CCK-8 assay
Anti-influenza activity against Influenza B virus Victoria/2/87 infected in HEK293T cells assessed as reduction in virus-induced cytopathic effect incubated for 48 hrs by CCK-8 assay
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[PMID: 38775356] |
| HEK-293T | EC50 |
0.332 μM
Compound: 11
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Anti-influenza activity against Influenza A virus A/WSN/33(H1N1) infected in HEK293T cells assessed as reduction in virus-induced cytopathic effect incubated for 48 hrs by CCK-8 assay
Anti-influenza activity against Influenza A virus A/WSN/33(H1N1) infected in HEK293T cells assessed as reduction in virus-induced cytopathic effect incubated for 48 hrs by CCK-8 assay
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[PMID: 38775356] |
| MDCK | CC50 |
>200 μM
Compound: 11
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Cytotoxicity against dog MDCK cells assessed as reduction in cell viability incubated for 48 hrs by CCK-8 assay
Cytotoxicity against dog MDCK cells assessed as reduction in cell viability incubated for 48 hrs by CCK-8 assay
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[PMID: 38775356] |
| MDCK | CC50 |
100 μM
Compound: 9r
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Cytotoxicity against MDCK cells by CCK-8 assay
Cytotoxicity against MDCK cells by CCK-8 assay
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[PMID: 37531746] |
| Vero | EC50 |
2.27 μM
Compound: 20r
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Antiviral activity against La Crosse virus infected in african green monkey Vero cells assessed as inhibition of virus-induced cytopathic effect by fluorometric analysis
Antiviral activity against La Crosse virus infected in african green monkey Vero cells assessed as inhibition of virus-induced cytopathic effect by fluorometric analysis
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[PMID: 22664128] |
Tenovin-3 downregulates both P-FAK and P-Src[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1011301-27-1
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Appearance Solid
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Molecular Weight 327.44
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Formula C18H21N3OS
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Color White to off-white
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SMILES
O=C(NC(NC1=CC=C(N)C=C1)=S)C2=CC=C(C(C)(C)C)C=C2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Solvent & Solubility
DMSO : ≥ 31 mg/mL (94.67 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.63 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.63 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Lain S, et al. Discovery, in vivo activity, and mechanism of action of a small-molecule p53 activator. Cancer Cell. 2008 May;13(5):454-63. [Content Brief]
[2]. Jifei Miao, et al. Nuclear HMGB1 promotes the phagocytic ability of macrophages. Exp Cell Res. 2020 Aug 1;393(1):112037. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.0540 mL | 15.2700 mL | 30.5399 mL | 76.3499 mL |
| 5 mM | 0.6108 mL | 3.0540 mL | 6.1080 mL | 15.2700 mL | |
| 10 mM | 0.3054 mL | 1.5270 mL | 3.0540 mL | 7.6350 mL | |
| 15 mM | 0.2036 mL | 1.0180 mL | 2.0360 mL | 5.0900 mL | |
| 20 mM | 0.1527 mL | 0.7635 mL | 1.5270 mL | 3.8175 mL | |
| 25 mM | 0.1222 mL | 0.6108 mL | 1.2216 mL | 3.0540 mL | |
| 30 mM | 0.1018 mL | 0.5090 mL | 1.0180 mL | 2.5450 mL | |
| 40 mM | 0.0763 mL | 0.3817 mL | 0.7635 mL | 1.9087 mL | |
| 50 mM | 0.0611 mL | 0.3054 mL | 0.6108 mL | 1.5270 mL | |
| 60 mM | 0.0509 mL | 0.2545 mL | 0.5090 mL | 1.2725 mL | |
| 80 mM | 0.0382 mL | 0.1909 mL | 0.3817 mL | 0.9544 mL |
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