JH530
Based on 1 Customer Validation
JH530 is an effective methuosis inducer that inhibits the triple-negative breast cancer (TNBC) cells proliferation by causing intracellular complete vacuolization. JH530 has anti-tumor activity and can be used for cancer research.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 98.99%
- No. CAS: 2928616-74-2
- Fòrmula: C22H24BrN7O2S
- Peso molecular:530.44
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Actividad biológica
Methuosis[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HCC1806 | IC50 |
0.7 μM
Compound: 5c; JH530
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Antiproliferative activity against human HCC1806 cells assessed as inhibition of cell proliferation incubated for 24 hrs by SRB assay
Antiproliferative activity against human HCC1806 cells assessed as inhibition of cell proliferation incubated for 24 hrs by SRB assay
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[PMID: 37212861] |
| HCC1937 | IC50 |
1.03 μM
Compound: 5c; JH530
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Antiproliferative activity against human HCC1937 cells assessed as inhibition of cell proliferation incubated for 24 hrs by SRB assay
Antiproliferative activity against human HCC1937 cells assessed as inhibition of cell proliferation incubated for 24 hrs by SRB assay
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[PMID: 37212861] |
| MDA-MB-468 | IC50 |
0.92 μM
Compound: 5c; JH530
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Antiproliferative activity against human MDA-MB-468 cells assessed as inhibition of cell proliferation incubated for 24 hrs by SRB assay
Antiproliferative activity against human MDA-MB-468 cells assessed as inhibition of cell proliferation incubated for 24 hrs by SRB assay
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[PMID: 37212861] |
JH530 (compound 5c) (1 μM or 2 μM; 24 hours) causes notable cellular morphological changes in HCC1806 cells, characterized by the accumulation of intracellular vacuoles, and scarcely affected the morphology of 184B5 cells and cell viability[1].
JH530 (0.5, 1.0, 1.5μM; 24 hours) causes cell death by methuosis[1].
JH530 (1 μM; 24 hours) effectively suppresses the proliferation of TNBC cells invitro[1].
JH530 (1.5 μM; 24 hours) causes the increase of Rab7 and Lamp1 expression in HCC1806 and MDA-MB-468[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HCC1806, HCC1937, MDA-MB-468 cells
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Concentration:1 μM
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Incubation Time:24 hours
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Result:Expressed remarkable anti-proliferative activities invitro, with the IC50s were 0.70 μM, 0.92 μM, and 1.03 μM for three TNBC cells HCC1806, MDA-MB-468, and HCC1937, respectively.
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Cell Line:HCC1806, 184B5
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Concentration:1 μM or 2 μM
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Incubation Time:24 hours
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Result:Exhibited notable cellular morphological changes at 1 μM, characterized by the accumulation of intracellular vacuoles in HCC1806 cells.
Scarcely affected the morphology of 184B5 cells and cell viability.
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Cell Line:HCC1806; MDA-MB-468
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Concentration:0, 0.5, 1.0, 1.5 μM
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Incubation Time:24 hours
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Result:Dose-dependently induced the increase of Rab7 and Lamp1 expression, and causes cell death by methuosis.
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Cell Line:HCC1806, HCC1937, MDA-MB-468 cells
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Concentration:1.5 μM
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Incubation Time:24 hours
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Result:Induced the increase of Rab7 and Lamp1 expression in HCC1806 and MDA-MB-468.
Induced the accumulation of vacuoles in most of the cell.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:HCC1806 cell xenograft mouse model [1]
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Dosage:2.5 mg/kg, 5.0 mg/kg
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Administration:Intraperitoneal injection (i.p.); once every 2 days
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Result:Inhibits HCC1806 tumor weight at 2.5 mg/kg significantly, while exhibit more apparent tumor suppressive effects at 5 mg/kg[1].
Chemical Information
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No. CAS 2928616-74-2
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Appearance Solid
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Peso molecular 530.44
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Fòrmula C22H24BrN7O2S
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Color Light yellow to yellow
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SMILES
O=C(C1=CC=CC(Br)=N1)NC2=C(OC)C=C(NC3=NC(N4CCNCC4)=NC=C3SC)C=C2
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
DMSO : 1.67 mg/mL (3.15 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureza y Documentación
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Ficha de datos (276 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.8852 mL | 9.4261 mL | 18.8523 mL | 47.1307 mL |
JH530 Related Classifications
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