rG4 mut_AHPC_PEG2
rG4 mut_AHPC_PEG2 is a structure-disruptive negative control PROTAC. rG4 mut_AHPC_PEG2 demonstrates that the RNA G-quadruplex conformation is an absolute prerequisite for the recruitment and degradation of DHX36 by retaining the E3-recruiting moiety (AHPC-PEG2) while disrupting the secondary structure of the nucleic acid moiety (mutated RNA G-quadruplex),.
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Almacenamiento:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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rG4 mut_AHPC_PEG2 (30 nM; 30 min) does not bind to RHAU53 peptide, confirming that G4 structure integrity is required for interaction with DHX36 fragments[1].
rG4 mut_AHPC_PEG2 (3 pmol; 4 h) does not colocalize with endogenous DHX36 in HeLa cells, confirming it cannot target DHX36 in cells[1].
rG4 mut_AHPC_PEG2 (250 nM; 24 h) does not degrade DHX36 protein in HeLa cells[1].
rG4 mut_AHPC_PEG2 (0-200 nM; 48 h) does not inhibit translation of the APP rG4 WT reporter construct in HeLa cells[1].
rG4 mut_AHPC_PEG2 (0-100 nM; 24 h) does not reduce native APP protein expression or degrade DHX36 in HEK 293T cells[1].
rG4 mut_AHPC_PEG2 (200 nM; 24 h) does not degrade DHX36 or reduce Gnai2 protein expression in C2C12 myoblast cells[1].
rG4 mut_AHPC_PEG2 (200 nM; 24 h) does not impair the proliferative capacity of C2C12 myoblast cells or skeletal muscle stem cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HeLa cells
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Concentration:3 pmol (FAM-labeled rG4 mut_AHPC_PEG2)
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Incubation Time:4 h (transfection)
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Result:Observed little to no colocalized fluorescent foci between FAM-labeled rG4 mut_AHPC_PEG2 and endogenous DHX36.
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Cell Line:HeLa cells
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Concentration:250 nM
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Incubation Time:24 h (transfection)
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Result:Caused no significant reduction in DHX36 protein levels compared to untreated or empty transfection controls.
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Cell Line:HEK 293T cells
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Concentration:0-100 nM
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Incubation Time:24 h (co-transfection)
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Result:Did not reduce native APP or Myc protein levels.
Did not degrade DHX36 protein.
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Cell Line:mouse C2C12 myoblast cells
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Concentration:200 nM
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Incubation Time:24 h (transfection)
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Result:Did not degrade DHX36 protein.
Did not reduce Gnai2 protein levels compared to controls.
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Cell Line:mouse C2C12 myoblast cells, skeletal muscle stem cells (SCs)
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Concentration:200 nM (rG4 mut_AHPC_PEG2); 10 μM (EdU)
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Incubation Time:24 h (rG4 mut_AHPC_PEG2 transfection); 3 h (EdU incubation)
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Result:Showed 55.06% EdU-positive C2C12 cells with no inhibition of proliferation.
Showed 3.70% EdU-positive SCs with no inhibition of proliferation.
Chemical Information
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Appearance Solid
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SMILES
[rG4 mut_AHPC_PEG2]
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Referencias
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
rG4 mut_AHPC_PEG2 Related Classifications
HY-185724 Related Classifications
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