Gibberellic acid
Based on 1 publication(s) in Google Scholar
Gibberellic acid (GA3) is a natural plant growth regulator with oral bioactivity. Gibberellic acid governs multiple plant developmental events, including seed germination, cell elongation, floral organ formation, and rice spikelet fertility. Gibberellic acid optimizes root morphology and secondary metabolite production in Echinacea purpurea hairy roots, and serves as a bioactive seed priming agent to improve chilling stress tolerance in chickpea. Gibberellic acid mediates modulation of enzyme activity and secondary metabolism in plants and exerts tissue-specific regulatory effects on antioxidant systems and lipid peroxidation in rats. Gibberellic acid can be used in studies of plant growth, metabolism, and abiotic stress responses.
For research use only. We do not sell to patients.
- Purity: 99.26%
- CAS No.: 77-06-5
- Formula: C19H22O6
- Molecular Weight:346.37
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Gibberellic acid
More
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| CA46 | IC50 |
>10000 nM
Compound: JRA-001
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Cytotoxicity against human CA46 cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay
Cytotoxicity against human CA46 cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay
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[PMID: 33062173] |
| HCC1599 | IC50 |
>10000 nM
Compound: JRA-001
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Cytotoxicity against human HCC1599 cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay
Cytotoxicity against human HCC1599 cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay
|
[PMID: 33062173] |
| HEK-293T | IC50 |
>10000 nM
Compound: JRA-001
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Cytotoxicity against human HEK293T cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay
Cytotoxicity against human HEK293T cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay
|
[PMID: 33062173] |
| Hs-578T | IC50 |
>10000 nM
Compound: JRA-001
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Cytotoxicity against human Hs-578T cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay
Cytotoxicity against human Hs-578T cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay
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[PMID: 33062173] |
| NCI-H446 | IC50 |
>10000 nM
Compound: JRA-001
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Cytotoxicity against human NCI-H446 cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay
Cytotoxicity against human NCI-H446 cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay
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[PMID: 33062173] |
| PSN1 | IC50 |
>10000 nM
Compound: JRA-001
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Cytotoxicity against human PSN1 cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay
Cytotoxicity against human PSN1 cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay
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[PMID: 33062173] |
| RCK8 | IC50 |
>10000 nM
Compound: JRA-001
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Cytotoxicity against human RC-K8 cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay
Cytotoxicity against human RC-K8 cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay
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[PMID: 33062173] |
| SUD4 | IC50 |
>10000 nM
Compound: JRA-001
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Cytotoxicity against human SU-DHL-4 cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay
Cytotoxicity against human SU-DHL-4 cells assessed as reduction in cell viability measured after 24 hrs by CellTiter-Glo luminescent assay
|
[PMID: 33062173] |
Gibberellic acid (GA3) (0.005-1.0 μM; 50 days) maximally enhances biomass, cell viability, secondary metabolite production (cichoric acid, caftaric acid, chlorogenic acid, lignin, anthocyanins), PAL activity, and free radical scavenging activity in light-grown Echinacea purpurea hairy root cultures, while 0.005 μM and 1.0 μM reduce these parameters relative to the 0.025 μM group[2].
Gibberellic acid maintains rice spikelet fertility and anther/pollen development by mediating SLR1 repressor degradation to activate GAMYB and downstream male reproductive genes, while GA deficiency induced by low temperature or GA signaling mutation impairs pollen development and results in male sterility and reduced spikelet fertility in rice[3].
Gibberellic acid (5-20 μM; continuous low-temperature stress treatment) improves stand establishment, shoot/root growth, relative water content, chlorophyll retention, and flower/branch formation, and reduces electrolyte leakage in both chilling-tolerant Çağatay and chilling-sensitive Akçin chickpea cultivars under low-temperature stress[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Echinacea purpurea hairy root cells
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Concentration:0.005 μM, 0.025 μM, 1.0 μM
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Incubation Time:0-50 days continuous incubation
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Result:Moderate 0.025 μM gibberellic acid significantly enhanced hairy root cell viability; low (0.005 μM) and high (1.0 μM) concentrations exhibited inhibitory effects on cell viability versus control group.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Sprague-Dawley albino (male, 4 months old, 250-300 g average weight)[1]
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Dosage:75 ppm (equivalent to ~2 mg/day per rat)
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Administration:p.o.; 25 days
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Result:Significantly increased malondialdehyde (MDA) content in spleen and lung tissues compared to controls.
Significantly increased superoxide dismutase (SOD) activity in spleen and kidney tissues compared to controls.
Significantly decreased glutathione reductase (GR) activity in spleen tissue and significantly increased in kidney tissue compared to controls.
Significantly increased glutathione-S-transferase (GST) activity in spleen and lung tissues, and significantly decreased in heart tissue compared to controls.
Chemical Information
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CAS No. 77-06-5
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Appearance Solid
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Molecular Weight 346.37
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Formula C19H22O6
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Color White to off-white
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SMILES
OC([C@H]1[C@@]([C@]23C)([H])[C@@](OC2=O)(C=C[C@@H]3O)[C@@](CC4)([H])[C@]1(C5)C[C@]4(O)C5=C)=O
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Synonyms
Gibberellin A3
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (1)
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Journal Impact Factor
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Most Recent
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Cell Res
Engineered poly(A)-surrogates for translational regulation and therapeutic biocomputation in mammalian cells. [Abstract]2024 Jan;34(1):31-46. PMID: 38172533
Solvent & Solubility
DMSO : 100 mg/mL (288.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 4 mg/mL (11.55 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (6.01 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (6.01 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (283 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Kwon CT, et al. Gibberellic Acid: A Key Phytohormone for Spikelet Fertility in Rice Grain Production. Int J Mol Sci. 2016 May 23;17(5). [Content Brief]
[3]. Celik I, et al. Abcisic acid and gibberellic acid cause increased lipid peroxidation and fluctuated antioxidant defense systems of various tissues in rats. J Hazard Mater. 2007 Sep 30;148(3):623-9. [Content Brief]
[4]. Abbasi BH, et al. Gibberellic acid increases secondary metabolite production in Echinacea purpurea hairy roots. Appl Biochem Biotechnol. 2012 Dec;168(7):2057-66. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 2.8871 mL | 14.4354 mL | 28.8709 mL | 72.1772 mL |
| 5 mM | 0.5774 mL | 2.8871 mL | 5.7742 mL | 14.4354 mL | |
| 10 mM | 0.2887 mL | 1.4435 mL | 2.8871 mL | 7.2177 mL | |
| DMSO | 15 mM | 0.1925 mL | 0.9624 mL | 1.9247 mL | 4.8118 mL |
| 20 mM | 0.1444 mL | 0.7218 mL | 1.4435 mL | 3.6089 mL | |
| 25 mM | 0.1155 mL | 0.5774 mL | 1.1548 mL | 2.8871 mL | |
| 30 mM | 0.0962 mL | 0.4812 mL | 0.9624 mL | 2.4059 mL | |
| 40 mM | 0.0722 mL | 0.3609 mL | 0.7218 mL | 1.8044 mL | |
| 50 mM | 0.0577 mL | 0.2887 mL | 0.5774 mL | 1.4435 mL | |
| 60 mM | 0.0481 mL | 0.2406 mL | 0.4812 mL | 1.2030 mL | |
| 80 mM | 0.0361 mL | 0.1804 mL | 0.3609 mL | 0.9022 mL | |
| 100 mM | 0.0289 mL | 0.1444 mL | 0.2887 mL | 0.7218 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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