1. NF-κB
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  3. Helenalin

Helenalin is an anti-inflammatory sesquiterpene lactone. Helenalin selectively inhibits transcription factor NF-κB by directly targeting p65. Helenalin has alkylating activity, targets the cysteine sulfhydryl groups in the p65 subunit of NF-κB, thereby inhibits its DNA binding.

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Helenalin Chemical Structure

Helenalin Chemical Structure

CAS No. : 6754-13-8

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Based on 4 publication(s) in Google Scholar

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Description

Helenalin is an anti-inflammatory sesquiterpene lactone. Helenalin selectively inhibits transcription factor NF-κB by directly targeting p65. Helenalin has alkylating activity, targets the cysteine sulfhydryl groups in the p65 subunit of NF-κB, thereby inhibits its DNA binding[1][2].

In Vitro

Helenalin (10 μM; 20-120 minutes) causes complete inhibition of NF-κB DNA binding after 80 minutes[1].
? The anti-inflammatory, anti-carcinogenic phytochemical, Helenalin is a potent inhibitor of periodic Skp2 accumulation, an F-box protein mediating SCF E3 ligase ubiquitylation and degradation of both CKIs during S phase progression[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: Jurkat T cells
Concentration: 10 μM
Incubation Time: 20-120 minutes
Result: Caused complete inhibition of NF-κB DNA binding after 80 minutes.
In Vivo

Helenalin (25 mg/kg; i.p.; 6 to 12 hours) administers to immature male ICR mice caused a rapid decrease in hepatic glutathione levels[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Immature male ICR mice[2]
Dosage: 25 mg/kg
Administration: i.p.; 6 to 12 hours
Result: Caused a rapid decrease in hepatic glutathione levels.
Molecular Weight

262.30

Formula

C15H18O4

CAS No.
Appearance

Solid

Color

White to yellow

SMILES

O[C@H]1[C@]2([H])C(C(O[C@@]2(C[C@H]([C@@]([H])([C@]13C)C=CC3=O)C)[H])=O)=C

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (381.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.8124 mL 19.0621 mL 38.1243 mL
5 mM 0.7625 mL 3.8124 mL 7.6249 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 98.87%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.8124 mL 19.0621 mL 38.1243 mL 95.3107 mL
5 mM 0.7625 mL 3.8124 mL 7.6249 mL 19.0621 mL
10 mM 0.3812 mL 1.9062 mL 3.8124 mL 9.5311 mL
15 mM 0.2542 mL 1.2708 mL 2.5416 mL 6.3540 mL
20 mM 0.1906 mL 0.9531 mL 1.9062 mL 4.7655 mL
25 mM 0.1525 mL 0.7625 mL 1.5250 mL 3.8124 mL
30 mM 0.1271 mL 0.6354 mL 1.2708 mL 3.1770 mL
40 mM 0.0953 mL 0.4766 mL 0.9531 mL 2.3828 mL
50 mM 0.0762 mL 0.3812 mL 0.7625 mL 1.9062 mL
60 mM 0.0635 mL 0.3177 mL 0.6354 mL 1.5885 mL
80 mM 0.0477 mL 0.2383 mL 0.4766 mL 1.1914 mL
100 mM 0.0381 mL 0.1906 mL 0.3812 mL 0.9531 mL
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Helenalin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Helenalin
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