2. Immunology/Inflammation Protein Tyrosine Kinase/RTK
  3. MHC Insulin Receptor IFNAR
  4. InsB (9-23)

InsB (9-23)  (Synonyms: Insulin B chain (9-23))

Cat. No.: HY-P1745 Purity: 98.98%
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InsB (9-23) (Insulin B chain (9-23)) is an HLA-DQ8-restricted insulin B-chain peptide composed of amino acid residues 9-23. InsB (9-23) serves as a major MHC II class-restricted antigen. InsB (9-23) supports the recognition and activation of T cells, stimulates the secretion of IFN-γ and cytokines, and induces cross-reactive immune responses. InsB (9-23)-specific CD4 T cells can initiate diabetes. InsB (9-23) can be used in research related to type 1 diabetes and autoimmune diabetes.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

InsB (9-23)

InsB (9-23) Chemical Structure

CAS No. : 163364-16-7

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Based on 1 publication(s) in Google Scholar

InsB (9-23) Related Antibodies

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Description

InsB (9-23) (Insulin B chain (9-23)) is an HLA-DQ8-restricted insulin B-chain peptide composed of amino acid residues 9-23. InsB (9-23) serves as a major MHC II class-restricted antigen. InsB (9-23) supports the recognition and activation of T cells, stimulates the secretion of IFN-γ and cytokines, and induces cross-reactive immune responses. InsB (9-23)-specific CD4 T cells can initiate diabetes. InsB (9-23) can be used in research related to type 1 diabetes and autoimmune diabetes[1][2][3].

IC50 & Target[1]

MHC II

 

In Vitro

InsB (9-23) (2.5 μM; 48 h) stimulates IFN-γ secretion by the insB:11-23-specific T1D#3 C8 human T cell clone from a type 1 diabetes patient when presented by HLA-DQ8-expressing HEK293 APCs[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

InsB (9-23) Related Antibodies

ELISA Assay[2]

Cell Line: insB:11-23-specific human T cell clone (T1D#3 C8) and HLA-DQ8-cis- or DQ8-trans-expressing HEK293 antigen-presenting cells
Concentration: 2.5 μM
Incubation Time: 48 h
Result: Stimulated IFN-γ secretion by the insB:11-23-specific human T cell clone, with lower activity observed relative to the hyperactive InsB:9-23R22E peptide variant.
In Vivo

InsB (9-23) (10 nmol/mouse; intra-footpad; single dose) immunization of male NOD mice elicits a cross-reactive T cell response to both InsB (9-23) and the microbial mimic hprt4-18 peptide, as shown by robust IL-2 secretion[2].
InsB (9-23) (100 μg; s.c.; once; 1 day after T-cell transfer) combined with adoptive transfer of InsB (9-23)-specific TCR-engineered human CD4+ T cells induces diabetes in 60% of streptozotocin-conditioned HLA-DQ8-Tg hu-mice, accompanied by pancreatic islet destruction and human T-cell infiltration[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: HLA-DQ8-transgenic NSG (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ) (6-12 weeks old, type 1 diabetes model via low-dose streptozotocin conditioning + adoptive transfer of autologous InsB (9-23)-specific TCR-engineered human CD4+ T cells + immunization with InsB (9-23))[3]
Dosage: 100 μg
Administration: s.c.; once (1 day after T-cell transfer)
Result: Induced hyperglycemia and diabetes in approximately 60% of mice by 28 days post-T-cell infusion.
Caused severe destruction of pancreatic islets and human CD3+ T-cell infiltration in diabetic mice.
Enabled detection of infused GFP+ InsB (9-23)-TCR-engineered CD4+ T cells in peripheral blood for at least 6 days, in spleen and liver for at least 3 weeks, in bone marrow for at least 5 weeks, and in pancreatic islets by 11 days post-infusion.
Molecular Weight

1645.90

Formula

C72H116N20O22S1
CAS No.
Appearance

Solid

Color

White to off-white

Sequence Shortening

SHLVEALYLVCGERG
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

  Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.  Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
Purity & Documentation
References
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InsB (9-23) Related Classifications

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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

InsB (9-23)163364-16-7Insulin B chain (9-23)MHCInsulin ReceptorIFNARMajor Histocompatibility ComplexInterferon-α/β receptorInterferon-alpha/beta receptorHLA-DQ8CD4 T cell clonesHEK293 APCsautoreactive T cell responsesI-Ag7 MHC class II moleculesInsB (9-23)-specific T-cell receptorspancreatic isletβ-celltype 1 diabetesNOD mouseInhibitorinhibitorinhibit

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