2. Immunology/Inflammation Protein Tyrosine Kinase/RTK
  3. MHC Insulin Receptor IFNAR
  4. InsB (9-23)

InsB (9-23)  (Synonyms: Insulin B chain (9-23))

Cat. No.: HY-P1745 Purity: 98.98%
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InsB (9-23) (Insulin B chain (9-23)) is an HLA-DQ8-restricted insulin B-chain peptide composed of amino acid residues 9-23. InsB (9-23) serves as a major MHC II class-restricted antigen. InsB (9-23) supports the recognition and activation of T cells, stimulates the secretion of IFN-γ and cytokines, and induces cross-reactive immune responses. InsB (9-23)-specific CD4 T cells can initiate diabetes. InsB (9-23) can be used in research related to type 1 diabetes and autoimmune diabetes.

For research use only. We do not sell to patients.

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InsB (9-23)

InsB (9-23) Chemical Structure

CAS No. : 163364-16-7

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InsB (9-23) Related Antibodies

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Description

InsB (9-23) (Insulin B chain (9-23)) is an HLA-DQ8-restricted insulin B-chain peptide composed of amino acid residues 9-23. InsB (9-23) serves as a major MHC II class-restricted antigen. InsB (9-23) supports the recognition and activation of T cells, stimulates the secretion of IFN-γ and cytokines, and induces cross-reactive immune responses. InsB (9-23)-specific CD4 T cells can initiate diabetes. InsB (9-23) can be used in research related to type 1 diabetes and autoimmune diabetes[1][2][3].

IC50 & Target[1]

MHC II

 

In Vitro

InsB (9-23) (2.5 μM; 48 h) stimulates IFN-γ secretion by the insB:11-23-specific T1D#3 C8 human T cell clone from a type 1 diabetes patient when presented by HLA-DQ8-expressing HEK293 APCs[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

InsB (9-23) Related Antibodies

ELISA Assay[2]

Cell Line: insB:11-23-specific human T cell clone (T1D#3 C8) and HLA-DQ8-cis- or DQ8-trans-expressing HEK293 antigen-presenting cells
Concentration: 2.5 μM
Incubation Time: 48 h
Result: Stimulated IFN-γ secretion by the insB:11-23-specific human T cell clone, with lower activity observed relative to the hyperactive InsB:9-23R22E peptide variant.
In Vivo

InsB (9-23) (10 nmol/mouse; intra-footpad; single dose) immunization of male NOD mice elicits a cross-reactive T cell response to both InsB (9-23) and the microbial mimic hprt4-18 peptide, as shown by robust IL-2 secretion[2].
InsB (9-23) (100 μg; s.c.; once; 1 day after T-cell transfer) combined with adoptive transfer of InsB (9-23)-specific TCR-engineered human CD4+ T cells induces diabetes in 60% of streptozotocin-conditioned HLA-DQ8-Tg hu-mice, accompanied by pancreatic islet destruction and human T-cell infiltration[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: HLA-DQ8-transgenic NSG (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ) (6-12 weeks old, type 1 diabetes model via low-dose streptozotocin conditioning + adoptive transfer of autologous InsB (9-23)-specific TCR-engineered human CD4+ T cells + immunization with InsB (9-23))[3]
Dosage: 100 μg
Administration: s.c.; once (1 day after T-cell transfer)
Result: Induced hyperglycemia and diabetes in approximately 60% of mice by 28 days post-T-cell infusion.
Caused severe destruction of pancreatic islets and human CD3+ T-cell infiltration in diabetic mice.
Enabled detection of infused GFP+ InsB (9-23)-TCR-engineered CD4+ T cells in peripheral blood for at least 6 days, in spleen and liver for at least 3 weeks, in bone marrow for at least 5 weeks, and in pancreatic islets by 11 days post-infusion.
Molecular Weight

1645.90

Formula

C72H116N20O22S1
CAS No.
Appearance

Solid

Color

White to off-white

Sequence Shortening

SHLVEALYLVCGERG
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 11.36 mg/mL (6.90 mM; ultrasonic and adjust pH to 10 with 1 M NaOH)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.6076 mL 3.0379 mL 6.0757 mL
5 mM 0.1215 mL 0.6076 mL 1.2151 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 0.6076 mL 3.0379 mL 6.0757 mL 15.1893 mL
5 mM 0.1215 mL 0.6076 mL 1.2151 mL 3.0379 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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InsB (9-23) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

InsB (9-23)163364-16-7Insulin B chain (9-23)MHCInsulin ReceptorIFNARMajor Histocompatibility ComplexInterferon-α/β receptorInterferon-alpha/beta receptorHLA-DQ8CD4 T cell clonesHEK293 APCsautoreactive T cell responsesI-Ag7 MHC class II moleculesInsB (9-23)-specific T-cell receptorspancreatic isletβ-celltype 1 diabetesNOD mouseInhibitorinhibitorinhibit

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