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  4. Iron(II) sulfate hydrate

Iron(II) sulfate hydrate is an iron(II) salt with oral activity, acting as an iron supplier, and is easily oxidized to iron(III) in water. Iron(II) sulfate hydrate induces apoptotic morphological changes in cancer cells, and promotes dose‑dependent iron accumulation in rats. Iron(II) sulfate hydrate can be used in studies of leukemia, breast cancer, iron deficiency, anemia, and restless legs syndrome.

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Iron(II) sulfate hydrate

Iron(II) sulfate hydrate Chemical Structure

CAS No. : 17375-41-6

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Iron(II) sulfate hydrate Related Antibodies

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Description

Iron(II) sulfate hydrate is an iron(II) salt with oral activity, acting as an iron supplier, and is easily oxidized to iron(III) in water. Iron(II) sulfate hydrate induces apoptotic morphological changes in cancer cells, and promotes dose‑dependent iron accumulation in rats. Iron(II) sulfate hydrate can be used in studies of leukemia, breast cancer, iron deficiency, anemia, and restless legs syndrome[1][2][3].

In Vitro

Iron (II) sulfate (16-1000 μM; 48 h) hydrate exerts dose-dependent growth inhibition in both K562 and T47D cells, with significant effects in K562 cells at ≥ 63 μM and strong, near-maximal inhibition in T47D cells at 1000 μM.[1].
Iron (II) sulfate (16-1000 μM; 48 h) hydrate causes a dose-dependent increase in absorbance at 490 nm in a cell-free environment, with significant increases at ≥ 63 μM[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Iron(II) sulfate hydrate Related Antibodies

Cell Viability Assay[1]

Cell Line: human chronic myelogenous leukaemia K562 cells, human breast carcinoma T47D cells
Concentration: 16 μM, 31 μM, 63 μM, 125 μM, 250 μM, 500 μM, 1000 μM
Incubation Time: 48 h
Result: Exhibited a growth inhibitory effect at 16-125 μM.
Appeared growth stimulatory at 1000 μM in the MTS assay.
Showed a dose-dependent increase in absorbance at 490 nm before adding MTS/PMS after 48 h of incubation.

Cell Cytotoxicity Assay[1]

Cell Line: human breast carcinoma T47D cells
Concentration: 1000 μM
Incubation Time: 48 h
Result: Induced loss of adherent property, cell rounding, and cell shrinkage in T47D cells, indicating cytotoxicity.
In Vivo

Iron (II) sulfate (2.84-11.54 mg Fe/kg body weight/day; oral dietary; daily; 31 or 61 days) hydrate accumulates in rat liver, spleen, and kidneys in a dose-dependent manner[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Sprague-Dawley Rats (male, 5-6 weeks old)[2]
Dosage: 35 mg Fe/kg diet (equivalent to 2.84 mg Fe/kg body weight/day); 70 mg Fe/kg diet (equivalent to 5.69 mg Fe/kg body weight/day); 140 mg Fe/kg diet (equivalent to 11.54 mg Fe/kg body weight/day)
Administration: oral dietary; daily; 31 or 61 days
Result: Increased non‑heme iron levels in a dose‑dependent manner in the liver, spleen, and kidneys of rats at 31 and 61 days.
Elevated liver non‑heme iron to 67.2, 89.0, and 116.1 μg Fe/g tissue; spleen non‑heme iron to 171.0, 213.0, and 285.6 μg Fe/g tissue; kidney non‑heme iron to 29.0, 30.3, and 31.5 μg Fe/g tissue in low‑, mid‑, and high‑dose groups at 31 days, respectively.
Significantly increased mean corpuscular hemoglobin to 1.30 fmol in the high‑dose group compared with the low‑dose group at 31 days.
Increased liver non‑heme iron to 105.6, 120.2, and 126.4 μg Fe/g tissue; spleen non‑heme iron to 1018.5, 1107.2, and 1126.4 μg Fe/g tissue; kidney non‑heme iron to 43.5, 49.0, and 48.0 μg Fe/g tissue in low‑, mid‑, and high‑dose groups at 61 days, respectively.
Significantly elevated total iron binding capacity to 99.6 mmol/l in the low‑dose group relative to mid‑ and high‑dose groups at 61 days.
Maintained plasma iron concentrations within normal ranges and never exceeded 28.8 μmol/l at any dose or duration.
Molecular Weight

169.92

Formula

FeH2O5S
CAS No.
SMILES

O=S(=O)=[Fe](=O)=O.O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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Iron(II) sulfate hydrate Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Iron(II) sulfate17375-41-6Biochemical Assay Reagentsgut microbiotaferritinK562 cellsbreast carcinomachronic myelogenous leukaemiarestless legs syndrometransferrin saturationabsolute iron deficiencyiron-deficiency anemiaT47D cellsInhibitorinhibitorinhibit

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