1. Alkaloids
  2. Kifunensine

Kifunensine (Synonyms: FR-900494)

Cat. No.: HY-19332 Purity: >98.0%
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Kifunensine, a potent and selective inhibitor of class I α-mannosidases isolated from Actinomycete, prevents α-mannosidases I from trimming mannose residues on glycoproteins. Kifunensine inhibits ERAD.

For research use only. We do not sell to patients.

Kifunensine Chemical Structure

Kifunensine Chemical Structure

CAS No. : 109944-15-2

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5 mg USD 300 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

Kifunensine, a potent and selective inhibitor of class I α-mannosidases isolated from Actinomycete, prevents α-mannosidases I from trimming mannose residues on glycoproteins. Kifunensine inhibits ERAD[1][2][3].

In Vitro

Kifunensine, an alkaloid from actinomycete Kitasatosporia kifunense 9482, is the most efficient nhibitor of a-mannosidase I, blocking N-glycan synthesis at an8GlcNAc2 (Man8) or Man9GlcNAc2 (Man9) stage[3].

RT-PCR[3].

Cell Line: Hybridoma cells expressing a human IgG1 monoclonal Antibody[3].
Concentration: 2 μg/mL.
Incubation Time: 4 days.
Result: Significantly reduced the lentil lectin binding.
Kifunensine is the most effective among the inhibitors tested in producing antibodies containing oligomannose residues without fucose.
In Vivo
Animal Model: BALB/c mice[3].
Dosage: 5 mg/kg.
Administration: IV via tail vein.
Result: There was no significant difference in the serum levels of anti-TEM B mAb generated from cells treated with or without kifunensine in the 7-day period.
Molecular Weight

232.19

Formula

C₈H₁₂N₂O₆

CAS No.

109944-15-2

SMILES
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 11.9 mg/mL (51.25 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.3068 mL 21.5341 mL 43.0682 mL
5 mM 0.8614 mL 4.3068 mL 8.6136 mL
10 mM 0.4307 mL 2.1534 mL 4.3068 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 1.19 mg/mL (5.13 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 1.19 mg/mL (5.13 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 1.19 mg/mL (5.13 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Keywords:

KifunensineFR-900494FR900494FR 900494OthersERADER StressInhibitorinhibitorinhibit

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Product Name:
Kifunensine
Cat. No.:
HY-19332
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