Kifunensine
Based on 16 publication(s) in Google Scholar
Kifunensine, a potent and selective inhibitor of class I α-mannosidases isolated from Actinomycete, prevents α-mannosidases I from trimming mannose residues on glycoproteins. Kifunensine inhibits ERAD.
For research use only. We do not sell to patients.
- Purity: 99.90%
- CAS No.: 109944-15-2
- Formula: C8H12N2O6
- Molecular Weight:232.19
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Kifunensine
More- Nat Commun. 2025 Dec 10. [Abstract]
- Adv Sci (Weinh). 2025 Jan 14:e2409105. [Abstract]
- Nat Struct Mol Biol. 2025 Aug 8. [Abstract]
- Int J Biol Macromol. 2024 Sep 17:135764. [Abstract]
- Protein Sci. 2025 Jun;34(6):e70145. [Abstract]
- Protein Sci. 2024 Jul;33(7):e5081. [Abstract]
- J Cell Physiol. 2024 Aug 11:e31403. [Abstract]
- J Biol Chem. 2023 Oct;299(10):105211. [Abstract]
- Development. 2022 Sep 1;149(17):dev200892. [Abstract]
- Glycobiology. 2026 May 22;36(7):cwag035. [Abstract]
- bioRxiv. 2026 Jun 18.
- University of Pennsylvania. 2026.
- bioRxiv. 2025 Aug 08.
- SSRN. 2025 Apr 24.
- bioRxiv. 2024 October 31.
- bioRxiv. 2023 Jul 6.
Customer Validation & Images
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Histological Imaging/Staining
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Biological Activity
Kifunensine, an alkaloid from actinomycete Kitasatosporia kifunense 9482, is the most efficient nhibitor of a-mannosidase I, blocking N-glycan synthesis at an8GlcNAc2 (Man8) or Man9GlcNAc2 (Man9) stage[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Hybridoma cells expressing a human IgG1 monoclonal Antibody[3].
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Concentration:2 μg/mL.
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Incubation Time:4 days.
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Result:Significantly reduced the lentil lectin binding.
Kifunensine is the most effective among the inhibitors tested in producing antibodies containing oligomannose residues without fucose.
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Animal Model:BALB/c mice[3].
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Dosage:5 mg/kg.
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Administration:IV via tail vein.
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Result:There was no significant difference in the serum levels of anti-TEM B mAb generated from cells treated with or without kifunensine in the 7-day period.
Chemical Information
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CAS No. 109944-15-2
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Appearance Solid
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Molecular Weight 232.19
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Formula C8H12N2O6
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Color White to off-white
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SMILES
O=C(N1[C@@](N2)([H])[C@@H](O)[C@@H](O)[C@H](O)[C@H]1CO)C2=O
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Synonyms
FR-900494
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Structure Classification
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Initial Source
actinomycete
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 2 years -20°C 1 year
Publications (16)
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Journal Impact Factor
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Most Recent
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Nat Commun
Chimeras co-targeting antigens and FcγRIIb trigger degradation of extracellular soluble proteins and pathological aggregates. [Abstract]2025 Dec 10. PMID: 41372196 -
Adv Sci (Weinh)
Mannose Promotes β-Amyloid Pathology by Regulating BACE1 Glycosylation in Alzheimer's Disease. [Abstract]2025 Jan 14:e2409105. PMID: 39807036
Kifunensine purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jan 14:e2409105. [Abstract]
Western blot analysis of BACE1 and Nicastrin expression was performed on CHO-APP cells treated with or without mannose (25 mM), with or without Kifunensine (10 μM).
Kifunensine purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jan 14:e2409105. [Abstract]
Immunofluorescence staining of Aβ in cerebrum from 6‐month‐old 5×FAD mice and 5×FAD+Kif (5 mg/kg, daily, iv, 7 days) mice.
Kifunensine purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jan 14:e2409105. [Abstract]
Western blot analysis of BACE1 and Nicastrin expression in cortex and hippocampus of 6‐month‐old 5×FAD mice and 5×FAD+Kif (5 mg/kg, daily, iv, 7 days) mice.
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Nat Struct Mol Biol
2025 Aug 8. PMID: 40781479 -
Int J Biol Macromol
2024 Sep 17:135764. PMID: 39299429 -
Protein Sci
Synthetic antibodies targeting EphA2 induce diverse signaling-competent clusters with differential activation. [Abstract]2025 Jun;34(6):e70145. PMID: 40411427 -
Protein Sci
Antigen-binding fragments with improved crystal lattice packing and enhanced conformational flexibility at the elbow region as crystallization chaperones. [Abstract]2024 Jul;33(7):e5081. PMID: 38924648 -
J Cell Physiol
A proton channel, Otopetrin 1 (OTOP1) is N-glycosylated at two asparagine residues in third extracellular loop. [Abstract]2024 Aug 11:e31403. PMID: 39129225 -
J Biol Chem
Golgi α-Mannosidases Regulate Cell Surface N-Glycan Type and Ectodomain Shedding of the Transmembrane Protease Corin. [Abstract]2023 Oct;299(10):105211. PMID: 37660903
Kifunensine purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2023 Oct;299(10):105211. [Abstract]
Western blot analysis of corin proteins in cell lysates from corin-expressing HEK293 cells treated with N-glycosylation inhibitors, including N-linked glycosylation inhibitor-1 (NGI-1), 1-deoxynojirimycin (1-DNJ), kifunensine (KIF, 10 μM, 48 h), and swainsonine (SWA). The activation-cleaved corin protease domain (corin-p) band is shown. Levels of β-tubulin in cell lysates were used to assess protein amounts in each sample.
Kifunensine purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2023 Oct;299(10):105211. [Abstract]
In cell lysates, the ratio of corin-p versus zymogen bands in 1-DNJ-, KIF- (10 μM, 48 h), and SWA-treated samples decreased to 35 ± 3, 35 ± 6, and 62 ± 17% of the vehicle, respectively (n = 3; p = 0.003, 1-DNJ versus the vehicle; p = 0.003, KIF versus the vehicle; p = 0.049, SWA versus the vehicle).
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Development
The functional specificity of ERECTA-family receptors in Arabidopsis stomatal development is ensured by molecular chaperones in the endoplasmic reticulum. [Abstract]2022 Sep 1;149(17):dev200892. PMID: 36052695 -
Glycobiology
Siglec-15 binds mucin-domain glycoproteins with extended glycans and marks an osteoclast-like, matrix remodeling myeloid state in human tumors. [Abstract]2026 May 22;36(7):cwag035. PMID: 42286920 -
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Solvent & Solubility
DMSO : 10 mg/mL (43.07 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.19 mg/mL (5.13 mM); Clear solution
This protocol yields a clear solution of ≥ 1.19 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (11.9 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.19 mg/mL (5.13 mM); Clear solution
This protocol yields a clear solution of ≥ 1.19 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (11.9 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (281 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[3]. Qun Zhou, et al. Development of a Simple and Rapid Method for Producing Non-Fucosylated Oligomannose Containing Antibodies With Increased Effector Function. Biotechnol Bioeng. 2008 Feb 15;99(3):652-65. [Content Brief]
[4]. Hyung Lim Elfrink, et al. Inhibition of Endoplasmic Reticulum Associated Degradation Reduces Endoplasmic Reticulum Stress and Alters Lysosomal Morphology and Distribution. Mol Cells. 2013 Apr 30; 35(4): 291–297. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 4.3068 mL | 21.5341 mL | 43.0682 mL | 107.6704 mL |
| 5 mM | 0.8614 mL | 4.3068 mL | 8.6136 mL | 21.5341 mL | |
| 10 mM | 0.4307 mL | 2.1534 mL | 4.3068 mL | 10.7670 mL | |
| 15 mM | 0.2871 mL | 1.4356 mL | 2.8712 mL | 7.1780 mL | |
| 20 mM | 0.2153 mL | 1.0767 mL | 2.1534 mL | 5.3835 mL | |
| 25 mM | 0.1723 mL | 0.8614 mL | 1.7227 mL | 4.3068 mL | |
| 30 mM | 0.1436 mL | 0.7178 mL | 1.4356 mL | 3.5890 mL | |
| 40 mM | 0.1077 mL | 0.5384 mL | 1.0767 mL | 2.6918 mL |
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