Cat. No.: HY-N0073

Sarsasapogenin

(Synonyms: Parigenin; Sarsagenin)

4 Cited Publications

고객리뷰

Based on 4 publication(s) in Google Scholar

Sarsasapogenin is a sapogenin from the Chinese medical herb Anemarrhena asphodeloides Bunge, with antidiabetic, anti-oxidative, anticancer and anti-inflamatory activities.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

Purity: 99.93%
CAS No.: 126-19-2
화학식: C₂₇H₄₄O₃
분자량:416.64

보관:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Sarsasapogenin Chemical Structure

CAS No.: 126-19-2

사이즈

가격

재고

수량

Sample (0.1mg)

In-stock

(In Japan)

Apply Now

견적 받기 Ask For Quote & Lead Time Consult sales personnel for more information Check price and availability 견적 받기 Ask For Quote & Lead Time Consult sales personnel for more information Check price and availability

대량구매 문의

Amount: USD 0.00

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (4)
용액&용해도
- In Vitro
- In Vivo
Protocol
순도&문서
References
Help & FAQs
Bioz

Biological Activity

제품 설명

Sarsasapogenin is a sapogenin from the Chinese medical herb Anemarrhena asphodeloides Bunge, with antidiabetic, anti-oxidative, anticancer and anti-inflamatory activities.

IC₅₀ & Target^[3]

NF-κB

All Endogenous Metabolite Isoforms :

All NF-κB Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	38.15 μM Compound: Sarsasapogenin	Cytotoxicity against human A549 cells assessed as decrease in cell proliferation after 48 hrs by MTT colorimetric assay Cytotoxicity against human A549 cells assessed as decrease in cell proliferation after 48 hrs by MTT colorimetric assay	[PMID: 28038327]
Bcap37	IC₅₀	>100 μM Compound: Sarsasapogenin	Cytotoxicity against human Bcap37 cells assessed as decrease in cell proliferation after 48 hrs by MTT colorimetric assay Cytotoxicity against human Bcap37 cells assessed as decrease in cell proliferation after 48 hrs by MTT colorimetric assay	[PMID: 28038327]
Hep 3B2	IC₅₀	>100 μM Compound: Sarsasapogenin	Cytotoxicity against human Hep3B cells assessed as decrease in cell proliferation after 48 hrs by MTT colorimetric assay Cytotoxicity against human Hep3B cells assessed as decrease in cell proliferation after 48 hrs by MTT colorimetric assay	[PMID: 28038327]
HepG2	IC₅₀	40.34 μM Compound: Sarsasapogenin	Cytotoxicity against human HepG2 cells assessed as decrease in cell proliferation after 48 hrs by MTT colorimetric assay Cytotoxicity against human HepG2 cells assessed as decrease in cell proliferation after 48 hrs by MTT colorimetric assay	[PMID: 28038327]
MCF7	IC₅₀	49.23 μM Compound: Sarsasapogenin	Cytotoxicity against human MCF7 cells assessed as decrease in cell proliferation after 48 hrs by MTT colorimetric assay Cytotoxicity against human MCF7 cells assessed as decrease in cell proliferation after 48 hrs by MTT colorimetric assay	[PMID: 28038327]
THP-1	IC₅₀	3.3 μM Compound: 3	Anticoagulant activity in human THP1 cells assessed as inhibition of TNF-alpha-stimulated tissue factor expression incubated 1 hr before TNFalpha challenge measured after 5 hrs Anticoagulant activity in human THP1 cells assessed as inhibition of TNF-alpha-stimulated tissue factor expression incubated 1 hr before TNFalpha challenge measured after 5 hrs	[PMID: 20561785]
U-251	IC₅₀	>100 μM Compound: Sarsasapogenin	Cytotoxicity against human U251 cells assessed as decrease in cell proliferation after 48 hrs by MTT colorimetric assay Cytotoxicity against human U251 cells assessed as decrease in cell proliferation after 48 hrs by MTT colorimetric assay	[PMID: 28038327]

In Vitro

Sarsasapogenin (20-80 μM) induces apoptosis via caspase-dependent mitochondrial pathway in heLa cells. Sarsasapogenin also induces apoptosis via the caspase-dependent mitochondrial apoptotic pathway. Sarsasapogenin (60 μM)-induced ROS generation triggers ER stress and mitochondrial dysfunction^[1]. Sarsasapogenin potently inhibits NF-κB and MAPK activation, as well as IRAK1, TAK1, and IκBα phosphorylation in LPS-stimulated macrophages. Moreover, Sarsasapogenin inhibits the binding of LPS to macrophage Toll-like receptor 4, and polarization of M2 to M1 macrophages^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Sarsasapogenin (20 and 40 mg/kg) significantly restores the sucrose preference deficit induced by olfactory bulbectomy (OB), and increases locomotor activity. Sarsasapogenin groups (20 and 40 mg/kg) have significantly lower immobility times, higher AChE protein expression levels than the OB group. Furthermore, Sarsasapogenin (20 and 40 mg/kg) groups have significantly higher α7-nAChR protein expression, and increases higher α4-nAChR protein expression levels compared to rats in the OB group^[2]. Sarsasapogenin (5 or 10 mg/kg, p.o.) inhibits TNBS-induced colon shortening and myeloperoxidase activity in mice, reducing NF-κB activation and interleukin (IL)-1β, tumor necrosis factor (TNF)-α, and IL-6 levels, while simultaneously increasing IL-10^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 126-19-2
Appearance Solid
분자량 416.64
화학식 C₂₇H₄₄O₃
Color White to off-white
SMILES

C[C@@H]1[C@]2(OC[C@@H](C)CC2)O[C@@]3([H])C[C@@]4([H])[C@]5([H])CC[C@]6([H])C[C@@H](O)CC[C@]6(C)[C@@]5([H])CC[C@]4(C)[C@]31[H]
Synonyms

Parigenin; Sarsagenin
Structure Classification
- Steroids
Initial Source
- Endogenous metabolite
선적

Room temperature in continental US; may vary elsewhere.

보관

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (4)

All

Sort by:

Journal Impact Factor

Phytother Res

Sarsasapogenin ameliorates diabetes-associated memory impairment and neuroinflammation through down-regulation of PAR-1 receptor. [Abstract]

2021 Jun;35(6):3167-3180. PMID: 33885189
Drug Des Devel Ther

Sarsasapogenin Suppresses RANKL-Induced Osteoclastogenesis in vitro and Prevents Lipopolysaccharide-Induced Bone Loss in vivo. [Abstract]

2020 Aug 24;14:3435-3447. PMID: 32943842
J Biochem Mol Toxicol

Sarsasapogenin Inhibits HCT116 and Caco-2 Cell Malignancy and Tumor Growth in a Xenograft Mouse Model of Colorectal Cancer by Inactivating MAPK Signaling. [Abstract]

2025 Mar;39(3):e70189. PMID: 40009047
PLoS One

Acid hydrolysis of saponins extracted in tincture. [Abstract]

2020 Dec 31;15(12):e0244654. PMID: 33382809

용액&용해도

In Vitro:

DMSO : 3.57 mg/mL (8.57 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 1 mg/mL (2.40 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.4002 mL

12.0008 mL

24.0015 mL

5 mM

0.4800 mL

2.4002 mL

4.8003 mL

10 mM

---

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass

Concentration

Volume

Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 0.5% CMC-Na/saline water

Solubility: 5 mg/mL (12.00 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage
mg/kg
Animal Weight (Per Animal)
g
Dosing Volume (Per Animal)
μL
Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

Protocol

Cell Assay^[1]

The effect of Sarsasapogenin on cell viability is measured by the MTT assay method. After exposure to Sarsasapogenin in the absence or presence of Z-VAD-FMK pretreatment for the indicated time, cells in 96-well plates are incubated with MTT. The formazan precipitate is dissolved in 200 μL of dimethyl sulfoxide, and the absorbance at 570 nm is measured using a Benchmark microplate reader.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration^[3]

The mice are randomly divided into 7 groups: normal control, TNBS-induced colitic control groups treated with vehicle, timosaponin AIII (5 or 10 mg/kg), Sarsasapogenin (5 or 10 mg/kg) or sulfasalazine (50 mg/kg). Each group consists of 6 mice. The colitis is induced by the intrarectal injection of 2.5% (w/v) TNBS solution (100 μL, dissolved in 50% ethanol) into the colon of anesthetized mice using a thin round-tip needle equipped with a syringe. The normal group is treated with vehicle alone instead of TNBS and test agents. If the injected TNBS solution is excreted, the mouse is excluded from the experiment. Timosaponin AIII or Sarsasapogenin (5 or 10 mg/kg) or sulfasalazine (50 mg/kg) dissolved in 2% tween 80 are orally administered once a day for 3 days after treatment with TNBS. The mice are killed 18 h after the final administration of test agents. The colon is quickly removed, opened longitudinally, and gently washed by PBS. Macroscopic evaluation of the colitis grade is scored (0, no ulcer and no inflammation; 1, no ulceration and local hyperemia; 2, ulceration with hyperemia; 3, ulceration and inflammation at one site only; 4, two or more sites of ulceration and inflammation; 5, ulceration extending more than 2 cm). The colons are stored at −80°C until used in the experiment for ELISA and immunoblotting.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

순도&문서

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (281 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Shen S, et al. Sarsasapogenin induces apoptosis via the reactive oxygen species-mediated mitochondrial pathway and ER stress pathway in HeLa cells. Biochem Biophys Res Commun. 2013 Oct 26.

[2]. Feng B, et al. Sarsasapogenin reverses depressive-like behaviors and nicotinic acetylcholine receptors induced by olfactory bulbectomy. Neurosci Lett. 2017 Feb 3;639:173-178. [Content Brief]

[3]. Lim SM, et al. Timosaponin AIII and its metabolite sarsasapogenin ameliorate colitis in mice by inhibiting NF-κB and MAPK activation and restoring Th17/Treg cell balance. Int Immunopharmacol. 2015 Apr;25(2):493-503. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
Ethanol / DMSO	1 mM	2.4002 mL	12.0008 mL	24.0015 mL	60.0038 mL
DMSO	5 mM	0.4800 mL	2.4002 mL	4.8003 mL	12.0008 mL

Keywords

Sarsasapogenin

4 Cited Publications

100 mg 견적 받기

Subscribe to the

MedChemExpress

Newsletter

상품명:
Cat. No.:
수량:
MCE Japan Authorized Agent:

상품명:
CAS No.:
Species:
Purity:
수량:

Sarsasapogenin

Immunology/Inflammation Compound Library

Metabolism/Protease Compound Library

NF-κB Signaling Compound Library

Stem Cell Signaling Compound Library

Natural Product Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Antioxidant Compound Library

Differentiation Inducing Compound Library

Anti-diabetic Compound Library

Lipid Compound Library

Oxygen Sensing Compound Library

Endoplasmic Reticulum Stress Compound Library

Pyroptosis Compound Library

Traditional Chinese Medicine Active Compound Library

Anti-Breast Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Anti-Obesity Compound Library

Transcription Factor-Targeted Library

Food-Sourced Compound Library

Anti-Liver Cancer Compound Library

Rare Diseases Drug Library

Anti-Cancer Natural Product Library

Antidepressant Compound Library

Anti-inflammatory Traditional Chinese Medicine Active Compound Library

Plant-Sourced Natural Product Library

Human Metabolite Library

Anti-Prostate Cancer Compound Library

Cancer Stem Cells Compound Library

Pain-Related Compound Library

Anti-Hematopathy Compound Library

Anti-Ovarian Cancer Compound Library

Radioprotector Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

Anti-Fibrosis Compound Library

Heat-Clearing and Detoxification Traditional Chinese Medicine Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Ancient Chinese Classical Formulas Traditional Chinese Medicine Active Compound Library

Mass Spectrometry Human Metabolite Library

Antitussive and Antiasthmatic Traditional Chinese Medicine Active Compound Library

Diarrhea-Related Traditional Chinese Medicine Active Compound Library

Mongolian Medicine Compound Library

Tonifying Traditional Chinese Medicine Monomer Compound Library

Lactylation Compound Library

Mass Spectrometry Natural Product Library

Publications Citing Use of MedChemExpress (MCE) Sarsasapogenin

All Endogenous Metabolite Isoforms

All NF-κB Isoforms

Biological Activity

Chemical Information

Publications (4)

용액&용해도

Protocol

순도&문서

References

Help & FAQs

Complete Stock Solution Preparation Table

Sarsasapogenin Related Classifications

Cat. No.: HY-N0073