L-Leucinol
Based on 1 Customer Validation
L-Leucinol ((+)-Leucinol) is a competitive aminopeptidase inhibitor with a Ki value of 17 μM. As a dietary intake inhibitor, L-Leucinol reduces the basal dietary leucine intake in rats via microinjection into the rat anterior piriform cortex.
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- Purity: 99.46%
- CAS No.: 7533-40-6
- 화학식: C6H15NO
- 분자량:117.19
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보관:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
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Biological Activity
L-leucinol interacts with the two metal ions in the binuclear active site of Co (II)-substituted aminopeptidase from Aeromonas proteolytica, altering the electronic symmetry or coordination geometry of Co2+ ions, which is evidenced by the red shift of absorption peaks and changes in molar absorptivity[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 7533-40-6
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Appearance Liquid (Density: 0.917 g/cm3)
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분자량 117.19
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화학식 C6H15NO
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Color Colorless to light yellow
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SMILES
CC(C)C[C@H](N)CO
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Synonyms
(+)-Leucinol
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
용액&용해도
DMSO : 100 mg/mL (853.32 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (21.33 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (21.33 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (270 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Bienvenue DL, et al. Inhibition of the aminopeptidase from Aeromonas proteolytica by L-leucinethiol: kinetic and spectroscopic characterization of a slow, tight-binding inhibitor-enzyme complex. J Inorg Biochem. 2000;78(1):43-54. [Content Brief]
[2]. Hao S, et al. Uncharged tRNA and sensing of amino acid deficiency in mammalian piriform cortex. Science. 2005;307(5716):1776-1778. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 8.5332 mL | 42.6658 mL | 85.3315 mL | 213.3288 mL |
| 5 mM | 1.7066 mL | 8.5332 mL | 17.0663 mL | 42.6658 mL | |
| 10 mM | 0.8533 mL | 4.2666 mL | 8.5332 mL | 21.3329 mL | |
| 15 mM | 0.5689 mL | 2.8444 mL | 5.6888 mL | 14.2219 mL | |
| 20 mM | 0.4267 mL | 2.1333 mL | 4.2666 mL | 10.6664 mL | |
| 25 mM | 0.3413 mL | 1.7066 mL | 3.4133 mL | 8.5332 mL | |
| 30 mM | 0.2844 mL | 1.4222 mL | 2.8444 mL | 7.1110 mL | |
| 40 mM | 0.2133 mL | 1.0666 mL | 2.1333 mL | 5.3332 mL | |
| 50 mM | 0.1707 mL | 0.8533 mL | 1.7066 mL | 4.2666 mL | |
| 60 mM | 0.1422 mL | 0.7111 mL | 1.4222 mL | 3.5555 mL | |
| 80 mM | 0.1067 mL | 0.5333 mL | 1.0666 mL | 2.6666 mL | |
| 100 mM | 0.0853 mL | 0.4267 mL | 0.8533 mL | 2.1333 mL |
L-Leucinol Related Classifications
HY-W015876 Related Classifications
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- L-Leucinol
- 7533-40-6
- (+)-Leucinol
- Aminopeptidase
- metal ions
- leucyl-tRNA synthetase
- Co(II)-substituted aminopeptidase from Aeromonas proteolytica
- anterior piriform cortex
- ATP exchange reaction
- aminopeptidase from Aeromonas proteolytica
- leucine activation
- rats
- dinuclear active site
- tRNA acylation
- Inhibitor
- inhibitor
- inhibit