2. Immunology/Inflammation
  3. Toll-like Receptor (TLR)
  4. L48H37

L48H37 is an analog of Curcumin (HY-N0005) with improved chemical stability. L48H37 is a potent and specific myeloid differentiation protein 2 (MD2) inhibitor and inhibits the interaction and signaling transduction of LPS-TLR4/MD2. L48H37 is used for the research of sepsis or lung injury treatment.

For research use only. We do not sell to patients.

L48H37 Chemical Structure

L48H37 Chemical Structure

CAS No. : 343307-76-6

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Based on 1 publication(s) in Google Scholar

L48H37 Related Antibodies

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Description

L48H37 is an analog of Curcumin (HY-N0005) with improved chemical stability. L48H37 is a potent and specific myeloid differentiation protein 2 (MD2) inhibitor and inhibits the interaction and signaling transduction of LPS-TLR4/MD2. L48H37 is used for the research of sepsis or lung injury treatment[1].

IC50 & Target[1]

TLR4

 

In Vitro

L48H37 inhibits LPS-induced inflammation, particularly TNF-α and IL-6 production and gene expression in mouse macrophages[1].
L48H37 (0-20 µM; 24 hours) decreases the viability of A549 and H460 cells with IC50 values of 5.3 µM and 2.3 µM, respectively, which is more effective compared to curcumin in lung cancer cells. It shows a low cytotoxicity on normal human lung epithelial cells (BEAS-2B) with IC50 of 21 μM[2].
L48H37 (1, 2, or 4 µM; 16 hours) dose‐dependently inhibited the expression of p‐Cdc2 and Cdc2, and increases the expression of p53. It also shows increased levels of cleaved poly (ADP‐ribosyl) polymerase (PARP) and reduced levels of anti‐apoptotic protein Bcl‐2 in H460 and A549 cells[2].
L48H37 (4 µM; 16 hours) rapidly induces intracellular ROS levels dose-dependently as detected by increased DCF levels in H460 and A549 cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

L48H37 Related Antibodies

Cell Viability Assay[2]

Cell Line: A549 and H460 cells; BEAS-2B cells
Concentration: 0.625, 1.25, 2.5, 5, 7.5, 10, and 20 µM
Incubation Time: 24 hours
Result: Inhibited lung cancer cells growth in a concentration-dependent manner.

Western Blot Analysis[2]

Cell Line: A549 and H460 cells
Concentration: 0.625, 1.25, 2.5, 5, 7.5, 10, and 20 µM
Incubation Time: 24 hours
Result: Decreased p‐Cdc2, Cdc2, and Bcl‐2 expression in 2 lung cancer cells.
In Vivo

L48H37 (intraperitoneal injection; 5 mg or 10 mg/kg; once daily; 11‐day ) inhibits H460 xenograft tumor growth and exhibits anti‐tumor activity in mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 5‐week‐old athymic BALB/cA nu/nu female mice (18‐22 g)[2]
Dosage: 5 mg or 10 mg/kg
Administration: Intraperitoneal injection; once daily; 11‐day
Result: Reduced tumor wet weights as compared to vehicle control.
Decreased the levels of p‐STAT3, and increased the levels of p‐EIF2α and ATF4 in vivo.
Exhibited no significant structural changes in mice.
Molecular Weight

483.55

Formula

C27H33NO7
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1/C(CN(C/C1=C\C2=CC(OC)=C(C(OC)=C2)OC)CC)=C/C3=CC(OC)=C(C(OC)=C3)OC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (103.40 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0680 mL 10.3402 mL 20.6804 mL
5 mM 0.4136 mL 2.0680 mL 4.1361 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.0680 mL 10.3402 mL 20.6804 mL 51.7010 mL
5 mM 0.4136 mL 2.0680 mL 4.1361 mL 10.3402 mL
10 mM 0.2068 mL 1.0340 mL 2.0680 mL 5.1701 mL
15 mM 0.1379 mL 0.6893 mL 1.3787 mL 3.4467 mL
20 mM 0.1034 mL 0.5170 mL 1.0340 mL 2.5850 mL
25 mM 0.0827 mL 0.4136 mL 0.8272 mL 2.0680 mL
30 mM 0.0689 mL 0.3447 mL 0.6893 mL 1.7234 mL
40 mM 0.0517 mL 0.2585 mL 0.5170 mL 1.2925 mL
50 mM 0.0414 mL 0.2068 mL 0.4136 mL 1.0340 mL
60 mM 0.0345 mL 0.1723 mL 0.3447 mL 0.8617 mL
80 mM 0.0259 mL 0.1293 mL 0.2585 mL 0.6463 mL
100 mM 0.0207 mL 0.1034 mL 0.2068 mL 0.5170 mL
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L48H37 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

L48H37343307-76-6Toll-like Receptor (TLR)InflammationAsthmalung injurySepsisTLR4/MD2IL-6TNF-αCurcuminInhibitorinhibitorinhibit

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