Cat. No.: HY-101923B

LYN-1604 dihydrochloride

7 Cited Publications

Customer Review

Based on 7 publication(s) in Google Scholar

LYN-1604 dihydrochloride is a potent UNC-51-like kinase 1 (ULK1) activator (EC₅₀=18.94 nM) for the research of triple negative breast cancer (TNBC).

For research use only. We do not sell to patients.

Purity: 99.55%
CAS No.: 2310109-38-5
Formula: C₃₃H₄₅Cl₄N₃O₂
Molecular Weight:657.54

Storage:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

LYN-1604 dihydrochloride Chemical Structure

CAS No.: 2310109-38-5

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Top Publications Citing

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (7)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

LYN-1604 dihydrochloride is a potent UNC-51-like kinase 1 (ULK1) activator (EC₅₀=18.94 nM) for the research of triple negative breast cancer (TNBC)^[1].

IC₅₀ & Target^[1]

ULK1

18.94 nM (EC₅₀)

All ULK Isoforms :

ULK1
ULK2

In Vitro

LYN-1604 is a potential ULK1 agonist (enzymatic activity=195.7% at 100 nM and IC₅₀=1.66 μM against MDA-MB-231 cells) ^[1].
LYN-1604 binds to wild-type ULK1 with a binding affinity in the nanomole range (K_D=291.4 nM) ^[1].
LYN-1604 (0.5, 1.0 and 2.0 μM) induces cell death via the ULK complex in MDA-MB-231 cells^[1].
LYN-1604 (0.5-2 μM, 24 hours) induces remarkable up-regulation of Beclin-1 and degradation of p62, as well as transformation of LC3-I to LC3-II in MDA-MB-231 cells^[1].
LYN-1604 induces ATG5-dependent autophagy via the ULK complex^[1].
LYN-1604 can also increase cleavage of caspase3 and induce apoptosis^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

MDA-MB-231 cells
Concentration:

0.5, 1.0 and 2.0 μM
Incubation Time:
Result:

Induced cell death. Autophagy ratio was increased in a dose-dependent manner.

Western Blot Analysis^[1]

Cell Line:

MDA-MB-231 cells
Concentration:

0, 0.5, 1, and 2 μM
Incubation Time:

24 hours
Result:

Induced remarkable up-regulation of Beclin-1 and degradation of p62, as well as transformation of LC3-I to LC3-II.

In Vivo

LYN-1604 (low dose, 25 mg/kg; median dose, 50 mg/kg; high dose, 100 mg/kg; intragastric administration once a day for 14 days) inhibits the growth of xenograft TNBC by targeting ULK1-modulated cell death^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

24 female nude mice (BALB/c, 6-8 weeks, 20-22 g)^[1]
Dosage:

Low dose, 25 mg/kg; median dose, 50 mg/kg; high dose, 100 mg/kg
Administration:

Intragastric administration; once a day for 14 days
Result:

Significantly inhibited the growth of xenograft MDA-MB-231 cells. The body weights of mice were stable. By the end of the experiment, the liver and spleen weight indexes of mice were slightly increased in parts of the groups, while the kidney weight index was not affected in all dose groups.

Chemical Information

CAS No. 2310109-38-5
Appearance Solid
Molecular Weight 657.54
Formula C₃₃H₄₅Cl₄N₃O₂
Color White to off-white
SMILES

[H]Cl.ClC1=CC=C(C(CN2CCN(C(CN(CC(C)C)CC(C)C)=O)CC2)OCC3=CC=C(C=CC=C4)C4=C3)C(Cl)=C1.[H]Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (7)

All WBIF

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Journal Impact Factor

Nat Aging

Reduced ULK1 links impaired autophagy and mitophagy to Alzheimer's disease pathology. [Abstract]

2026 May 14. PMID: 42135576
J Transl Med

Pbk positively regulates myoblast differentiation and muscle regeneration via enhancing AMPK/ULK1 mediated myogenic autophagy. [Abstract]

2025 Oct 21;23(1):1144. PMID: 41121382

LYN-1604 dihydrochloride purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Oct 21;23(1):1144. [Abstract]

C2C12 cells treated with or without HI-TOPK-032 (2 μM) and LYN-1604 (2 μM) were induced to differentiation for 3 days. After that, cells were harvested and subjected to western blot to detect and quantify the protein levels of MyHC and autophagy related genes.

LYN-1604 dihydrochloride purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Oct 21;23(1):1144. [Abstract]

C2C12 cells treated with or without HI-TOPK-032 (2 μM) and LYN-1604 (2 μM) were induced to differentiation for 3 days. Then cells were fixed and stained for MyHC (green) and DAPI (blue) to detect and quantify the differentiation index and fusion index of differentiating C2C12 cells.
Antioxidants (Basel)

Hyperosmolarity-Induced Oxidative Stress Leads to Senescence in Human Corneal Epithelial Cells (HCEPC) via DNA Damage, Metabolic Disturbance and Mitophagy Decline. [Abstract]

2025 Nov 19;14(11):1381. PMID: 41300537
Med Oncol

ULK1-driven autophagy modulation alters tumor-promoting pathways in triple-negative breast cancer. [Abstract]

2026 Mar 3;43(4):163. PMID: 41774346
Cell Signal

microRNA-125b-1-3p mediates autophagy via the RRAGD/mTOR/ULK1 signaling pathway and mitigates atherosclerosis progression. [Abstract]

2024 Jun:118:111136. PMID: 38471617
Biochem J

Conservation of structure, function and inhibitor binding in UNC-51-like kinase 1 and 2 (ULK1/2). [Abstract]

2019 Mar 12;476(5):875-887. PMID: 30782972
Oxid Med Cell Longev

ULK1 Suppresses Osteoclast Differentiation and Bone Resorption via Inhibiting Syk-JNK through DOK3. [Abstract]

2021 Nov 15:2021:2896674. PMID: 34820053

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (76.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 50 mg/mL (76.04 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.5208 mL

7.6041 mL

15.2082 mL

5 mM

0.3042 mL

1.5208 mL

3.0416 mL

10 mM

0.1521 mL

0.7604 mL

1.5208 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (3.80 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (3.80 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (281 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Zhang L, et al. Discovery of a small molecule targeting ULK1-modulated cell death of triple negative breast cancer in vitro and in vivo. Chem Sci. 2017 Apr 1;8(4):2687-2701. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO / H₂O	1 mM	1.5208 mL	7.6041 mL	15.2082 mL	38.0205 mL
	5 mM	0.3042 mL	1.5208 mL	3.0416 mL	7.6041 mL
	10 mM	0.1521 mL	0.7604 mL	1.5208 mL	3.8021 mL
	15 mM	0.1014 mL	0.5069 mL	1.0139 mL	2.5347 mL
	20 mM	0.0760 mL	0.3802 mL	0.7604 mL	1.9010 mL
	25 mM	0.0608 mL	0.3042 mL	0.6083 mL	1.5208 mL
	30 mM	0.0507 mL	0.2535 mL	0.5069 mL	1.2674 mL
	40 mM	0.0380 mL	0.1901 mL	0.3802 mL	0.9505 mL
	50 mM	0.0304 mL	0.1521 mL	0.3042 mL	0.7604 mL
	60 mM	0.0253 mL	0.1267 mL	0.2535 mL	0.6337 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

LYN-1604 dihydrochloride

7 Cited Publications

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LYN-1604 dihydrochloride

Bioactive Compound Library Max

Bioactive Compound Library

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Anti-Cancer Compound Library

Autophagy Compound Library

Anti-Breast Cancer Compound Library

Targeted Diversity Library

Highly Selective Activators Library

Cell Death Library

Mitophagy Compound Library

MCE Bioactive Compound Library

Protein Kinase Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) LYN-1604 dihydrochloride

All ULK Isoforms

Biological Activity

Chemical Information

Publications (7)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

LYN-1604 dihydrochloride

Bioactive Compound Library Max

Bioactive Compound Library

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Anti-Cancer Compound Library

Autophagy Compound Library

Anti-Breast Cancer Compound Library

Targeted Diversity Library

Highly Selective Activators Library

Cell Death Library

Mitophagy Compound Library

MCE Bioactive Compound Library

Protein Kinase Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) LYN-1604 dihydrochloride

All ULK Isoforms

Biological Activity

Chemical Information

Publications (7)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

LYN-1604 dihydrochloride Related Classifications

HY-101923B Related Classifications