2. Apoptosis
  3. Apoptosis
  4. Methyl (E)-cinnamate

Methyl (E)-cinnamate  (Synonyms: Methyl (E)-3-phenylpropenoate)

Cat. No.: HY-W067056 Purity: 99.87%
COA
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Handling Instructions Technical Support

Methyl (E)-cinnamate (Methyl (E)-3-phenylpropenoate) is a natural flavor compound with anti-inflammatory properties found in Alpinia katsumadai Hayata. Methyl (E)-cinnamate induces apoptosis via reduced anti-apoptotic protein expression, increased TUNEL-positive cells, and apoptotic morphological changes. Methyl (E)-cinnamate can be used for the research of abnormalities of osteoblast function in bone diseases.

For research use only. We do not sell to patients.

Methyl (E)-cinnamate

Methyl (E)-cinnamate Chemical Structure

CAS No. : 1754-62-7

Size Price Stock Quantity
Solid or liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid or liquid
100 g In-stock
500 g In-stock
1 kg In-stock

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Customer Review

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Other Forms of Methyl (E)-cinnamate:

Methyl (E)-cinnamate Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Methyl (E)-cinnamate (Methyl (E)-3-phenylpropenoate) is a natural flavor compound with anti-inflammatory properties found in Alpinia katsumadai Hayata. Methyl (E)-cinnamate induces apoptosis via reduced anti-apoptotic protein expression, increased TUNEL-positive cells, and apoptotic morphological changes. Methyl (E)-cinnamate can be used for the research of abnormalities of osteoblast function in bone diseases[1].

In Vitro

Methyl (E)-cinnamate (1-100 μM; 24-48 h) reduces cell viability and induces dose-dependent morphological shrinkage in pre-osteoblast MC3T3-E1 cells[1].
Methyl (E)-cinnamate (10-30 μM; 24 h) upregulates cleaved PARP and cleaved caspase-3, and downregulates Survivin and Bcl-2, in pre-osteoblast MC3T3-E1 cells[1].
Methyl (E)-cinnamate (10-30 μM; 24 h) dose-dependently inhibits ERK1/2 phosphorylation, and inhibits JNK and p38 phosphorylation at 30 μM, in pre-osteoblast MC3T3-E1 cells after 24 h of treatment[1].
Methyl (E)-cinnamate (10-30 μM; 24 h) induces dose-dependent DNA fragmentation, measured as increased TUNEL-positive cells, in pre-osteoblast MC3T3-E1 cells[1].
Methyl (E)-cinnamate (10-30 μM; 24 h) dose-dependently reduces cell migration rate in pre-osteoblast MC3T3-E1 cells[1].
Methyl (E)-cinnamate (10-30 μM; 7 days) dose-dependently suppresses osteoblast differentiation[1].
Methyl (E)-cinnamate (10-30 μM; 7 days) dose-dependently downregulates mRNA expression of osteogenic markers ALP, OCN, and OPN in pre-osteoblast MC3T3-E1 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Methyl (E)-cinnamate Related Antibodies

Cell Viability Assay[1]

Cell Line: MC3T3-E1
Concentration: 1 μM; 10 μM; 30 μM; 100 μM
Incubation Time: 24 h; 48 h
Result: Significantly inhibited cellular proliferation in a time- and dose-dependent manner, with statistically significant reductions in viability observed at 10, 30, and 100 μM after 24 h, and at 10, 30, and 100 μM after 48 h.
Induced dose-dependent morphological changes, including cell shrinkage into small, protruding shapes.

Western Blot Analysis[1]

Cell Line: MC3T3-E1
Concentration: 10 μM; 30 μM
Incubation Time: 24 h
Result: Significantly increased the levels of cleaved PARP and cleaved caspase-3 in a dose-dependent manner, with 10 μM and 30 μM both producing statistically significant increases relative to control.
Significantly reduced the expression of anti-apoptotic proteins Survivin and Bcl-2 in a dose-dependent manner, with both 10 μM and 30 μM producing statistically significant decreases relative to control.\nSignificantly inhibited the phosphorylation of ERK1/2 in a dose-dependent manner, with statistically significant reductions observed at both 10 μM and 30 μM relative to control.
Significantly reduced phosphorylation of JNK and p38 only at the 30 μM concentration, with statistically significant decreases relative to control.

Cell Migration Assay [1]

Cell Line: MC3T3-E1
Concentration: 10 μM; 30 μM
Incubation Time: 24 h
Result: Significantly suppressed cell migration rate in a dose-dependent manner, with 10 μM reducing migration to ~80% of control and 30 μM reducing migration to ~55% of control, both statistically significant relative to control.

Cell Differentiation Assay[1]

Cell Line: MC3T3-E1
Concentration: 10 μM; 30 μM
Incubation Time: 7 days
Result: Significantly decreased OS-induced osteoblast differentiation, as shown by reduced ALP staining intensity and reduced ALP activity in a dose-dependent manner.
Reduced ALP activity to ~9 nmol at 10 μM and ~7 nmol at 30 μM, compared to ~15 nmol in OS-treated control cells, with both concentrations showing statistically significant reductions relative to OS control.

Real Time qPCR[1]

Cell Line: MC3T3-E1
Concentration: 10 μM; 30 μM
Incubation Time: 7 days
Result: Significantly suppressed the mRNA levels of early and late osteogenic markers ALP, OCN, and OPN in a dose-dependent manner, with statistically significant reductions observed relative to OS control for all markers at both 10 μM and 30 μM.
Parmacokinetics
Species Dose Route CL Vss T1/2 F
Mice[1] 1 mg/kg i.v. 106 mL/min/kg 3.45 L/kg 0.37 h /
Mice[1] 10 mg/kg p.o. / / / 62 %
Mice[1] 45 mg/kg p.o. / / / 134 %
Mice[1] 90 mg/kg p.o. / / / 189 %
Mice[1] 200 mg/kg p.o. / / / 296 %
Mice[1] 400 mg/kg p.o. / / / 314 %
Molecular Weight

162.19

Formula

C10H10O2
CAS No.
Appearance

<34°C Solid,>38°C Liquid

Color

Colorless to off-white

SMILES

O=C(OC)/C=C/C1=CC=CC=C1
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Store at room temperature 3 years

In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (616.56 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.1656 mL 30.8280 mL 61.6561 mL
5 mM 1.2331 mL 6.1656 mL 12.3312 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 5 mg/mL (30.83 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 5 mg/mL (30.83 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.87%

SDS (393 KB)

COA (246 KB) HNMR (136 KB) GC (310 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 6.1656 mL 30.8280 mL 61.6561 mL 154.1402 mL
5 mM 1.2331 mL 6.1656 mL 12.3312 mL 30.8280 mL
10 mM 0.6166 mL 3.0828 mL 6.1656 mL 15.4140 mL
15 mM 0.4110 mL 2.0552 mL 4.1104 mL 10.2760 mL
20 mM 0.3083 mL 1.5414 mL 3.0828 mL 7.7070 mL
25 mM 0.2466 mL 1.2331 mL 2.4662 mL 6.1656 mL
30 mM 0.2055 mL 1.0276 mL 2.0552 mL 5.1380 mL
40 mM 0.1541 mL 0.7707 mL 1.5414 mL 3.8535 mL
50 mM 0.1233 mL 0.6166 mL 1.2331 mL 3.0828 mL
60 mM 0.1028 mL 0.5138 mL 1.0276 mL 2.5690 mL
80 mM 0.0771 mL 0.3854 mL 0.7707 mL 1.9268 mL
100 mM 0.0617 mL 0.3083 mL 0.6166 mL 1.5414 mL
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Methyl (E)-cinnamate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Methyl (E)-cinnamate1754-62-7Methyl (E)-3-phenylpropenoateApoptosisJNKpre-osteoblastcaspase-3ERK1/2p38MAPK signaling pathwayapoptosisALPPARPMC3T3-E1 cellsInhibitorinhibitorinhibit

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