2. PROTAC Cell Cycle/DNA Damage
  3. RIBOTAC G-quadruplex DNA/RNA Synthesis
  4. MJ-NR-27

MJ-NR-27 is a bifunctional small molecule of ribonuclease-targeting chimera (RIBOTAC) that targets NRAS mRNA containing a G-quadruplex structure. MJ-NR-27 uses RNase L ligand 3 (HY-177030) as the RNase L ligand, RNA binder 4 (HY-183981) as the RNA binder, and Bis-PEG3-acid (HY-126891) as the linker. MJ-NR-27 achieves target RNA degradation by recruiting ribonuclease RNase L, and significantly induces morphological changes in tumor cells. MJ-NR-27 can be used in cancer research.

For research use only. We do not sell to patients.

MJ-NR-27

MJ-NR-27 Chemical Structure

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MJ-NR-27 Related Antibodies

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RNASE L DNA Polymerases RNA Polymerases Helicases DNA Ligase
Description

MJ-NR-27 is a bifunctional small molecule of ribonuclease-targeting chimera (RIBOTAC) that targets NRAS mRNA containing a G-quadruplex structure. MJ-NR-27 uses RNase L ligand 3 (HY-177030) as the RNase L ligand, RNA binder 4 (HY-183981) as the RNA binder, and Bis-PEG3-acid (HY-126891) as the linker. MJ-NR-27 achieves target RNA degradation by recruiting ribonuclease RNase L, and significantly induces morphological changes in tumor cells. MJ-NR-27 can be used in cancer research[1].
In Vitro

MJ-NR-27 (compound 5) (10 μM; 24-72 h) reduces G4-containing NRAS mRNA levels by ~25% in MDA-MB-231 human breast adenocarcinoma cells, without affecting total NRAS mRNA levels[1].
MJ-NR-27 (10-40 μM; 24-72 h) exhibits marginal G4-containing NRAS mRNA degradation activity in MCF-7 human breast adenocarcinoma cells[1].
MJ-NR-27 (1-10 μM; 48 h) does not significantly alter NRAS protein expression in MCF-7 human breast adenocarcinoma cells[1].
MJ-NR-27 (0.46-46 μM; 72 h) exhibits no significant cytotoxicity in MCF-7 and MDA-MB-231 human breast adenocarcinoma cells after 72 h of incubation[1].
MJ-NR-27 (3-50 μM; 20 h) induces concentration-dependent cellular morphological changes in U2OS human osteosarcoma cells, with induction values ranging from 13% at 3 μM to 53.7% at 50 μM, and represents a new biological cluster with unique modes of action[1].
MJ-NR-27 (10 μM; 48 h) alters the transcriptomic profile of MCF-7 human breast adenocarcinoma cells, with significant upregulation of 5 genes and downregulation of 11 genes, including multiple cancer-associated targets[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

MJ-NR-27 Related Antibodies

Real Time qPCR[1]

Cell Line: MDA-MB-231 human breast adenocarcinoma cells
Concentration: 10 μM
Incubation Time: 24 h, 48 h, 72 h
Result: Reduced G4-NRAS mRNA levels by approximately 25% compared to vehicle control.
Showed no significant effect on total NRAS mRNA levels.

Real Time qPCR[1]

Cell Line: MCF-7 human breast adenocarcinoma cells
Concentration: 10 μM, 40 μM
Incubation Time: 24 h, 48 h, 72 h
Result: Showed marginal G4-NRAS mRNA degradation activity, consistent with results observed in MDA-MB-231 cells.

Western Blot Analysis[1]

Cell Line: MCF-7 human breast adenocarcinoma cells
Concentration: 1 μM, 10 μM
Incubation Time: 48 h
Result: Showed no significant changes in NRAS protein levels relative to vehicle control.
Molecular Weight

1071.29

Formula

C60H66N10O7S
SMILES

CNC([C@H](C1=CC=CC=C1)N(C)CC2=CC=C(C3=CC(C4=C(NC(CCOCCOCCOCCC(N5CCN(CCNC6=NC(C7=NN=C(C8=CC=C(C)C=C8)O7)=NC9=C6C=CC=C9)CC5)=O)=O)C=CS4)=CC=C3)C=C2)=O
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Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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MJ-NR-27 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MJ-NR-27RIBOTACG-quadruplexDNA/RNA SynthesisRibonuclease-targeting Chimeratranscriptomic profileNRAS mRNANRAS proteinhuman osteosarcoma cellsbreast cancer cellsU2OS human osteosarcoma cellscancer-associated targetsMCF-7 human breast adenocarcinoma cellsMDA-MB-231 human breast adenocarcinoma cellstotal NRAS mRNAInhibitorinhibitorinhibit

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