MMAF (Synonyms: Monomethylauristatin F)

Cat. No.: HY-15579G: Data Sheet
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Handling Instructions
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MMAF (Monomethylauristatin F) GMP is a GMP grade MMAF (HY-15579). GMP small molecules works appropriately as an auxiliary reagent for cell therapy manufacture. MMAF (Monomethylauristatin F) is a potent tubulin polymerization inhibitor and is used as a antitumor agent. MMAF (Monomethylauristatin F) is widely used as a cytotoxic component of antibody-drug conjugates (ADCs) such as vorsetuzumab mafodotin and SGN-CD19A.

For research use only. We do not sell to patients.

MMAF Chemical Structure

CAS No. : 745017-94-1

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This product is a controlled substance and not for sale in your territory.

Other Forms of MMAF:

MMAF sodium (GMP) Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

IC50: 119 nM (Cytotoxicity, Karpas 299 cell), 105 nM (Cytotoxicity, H3396 cell), 257 nM (Cytotoxicity, 786-O cell), 200 nM (Cytotoxicity, Caki-1, cell)^[4]

Cellular Effect

Cell Line	Type	Value	Description	References
BT-474	GI₅₀	0.24 nM Compound: 6-OMe, MMAF-OMe	Cytotoxicity against human BT474 cells assessed as cell viability incubated fore 4 days by MTS assay Cytotoxicity against human BT474 cells assessed as cell viability incubated fore 4 days by MTS assay	[PMID: 25431858]
BT-474	IC₅₀	8.8 nM Compound: MMAF	Cytotoxicity against human BT-474 cells assessed as reduction in cell viability incubated for 96 hrs by XTT assay Cytotoxicity against human BT-474 cells assessed as reduction in cell viability incubated for 96 hrs by XTT assay	[PMID: 34676042]
H3396	IC₅₀	105 nM Compound: 39; MMAF	Cytotoxicity against human H3396 cells assessed as cell growth inhibition Cytotoxicity against human H3396 cells assessed as cell growth inhibition	[PMID: 35072477]
H3396	IC₅₀	105 nM Compound: 4; MMAF	Cytotoxicity against human H3396 cells assessed as inhibition of cell growth incubated for 96 hrs by Alamar blue assay Cytotoxicity against human H3396 cells assessed as inhibition of cell growth incubated for 96 hrs by Alamar blue assay	[PMID: 39068862]
HCT-116	IC₅₀	2.88 μM Compound: 39; MMAF	Cytotoxicity against human HCT-116 cells assessed as reduction inc cell viability Cytotoxicity against human HCT-116 cells assessed as reduction inc cell viability	[PMID: 35072477]
HL-60	IC₅₀	137 nM Compound: 39; MMAF	Cytotoxicity against P-gp negative human HL-60 cells assessed as reduction inc cell viability Cytotoxicity against P-gp negative human HL-60 cells assessed as reduction inc cell viability	[PMID: 35072477]
HT-29	IC₅₀	10 nM Compound: 39; MMAF	Cytotoxicity against human HT-29 cells assessed as cell growth inhibition Cytotoxicity against human HT-29 cells assessed as cell growth inhibition	[PMID: 35072477]
MDA-MB-361	GI₅₀	0.21 nM Compound: 6-OMe, MMAF-OMe	Cytotoxicity against human MDA-MB-361 cells assessed as cell viability incubated fore 4 days by MTS assay Cytotoxicity against human MDA-MB-361 cells assessed as cell viability incubated fore 4 days by MTS assay	[PMID: 25431858]
NCI-N87	GI₅₀	0.44 nM Compound: 6-OMe, MMAF-OMe	Cytotoxicity against human NCI-N87 cells assessed as cell viability incubated fore 4 days by MTS assay Cytotoxicity against human NCI-N87 cells assessed as cell viability incubated fore 4 days by MTS assay	[PMID: 25431858]
SK-BR-3	IC₅₀	5.3 nM Compound: MMAF	Cytotoxicity against human SKBR3 cells assessed as reduction in cell viability incubated for 96 hrs by XTT assay Cytotoxicity against human SKBR3 cells assessed as reduction in cell viability incubated for 96 hrs by XTT assay	[PMID: 34676042]

In Vitro

MMAF inhibits anaplastic large cell lymphoma Karpas 299, breast carcinoma H3396, renal cell carcinoma 786-O and Caki-1 cells with IC₅₀s of 119, 105, 257 and 200 nM in vitro cytotoxicity assay^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

The maximum tolerated dose (MTD) in mice of MMAF (Monomethylauristatin F) (>16 mg/kg) is much higher than MMAE (Monomethylauristatin E) (1 mg/kg). cAC10-L1-MMAF₄ has an MTD of 50 mg/kg in mice and 15 mg/kg in rats. The corresponding cAC10-L4-MMAF₄ ADC was much less toxic, having MTDs in mice and rats of >150 mg/ kg and 90 mg/kg in rats, respectively^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

731.96

Formula

C₃₉H₆₅N₅O₈

CAS No.

745017-94-1

Appearance

Solid

Color

White to off-white

SMILES

CC(C)[C@H](NC)C(N[C@H](C(N([C@@H]([C@@H](C)CC)[C@H](OC)CC(N1CCC[C@@]1([H])[C@H](OC)[C@@H](C)C(N[C@H](C(O)=O)CC2=CC=CC=C2)=O)=O)C)=O)C(C)C)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Data Sheet (275 KB) SDS (252 KB) Handling Instructions (2659 KB)

References

[1]. Doronina SO, et al. Enhanced activity of monomethylauristatin F through monoclonal antibody delivery: effects of linker technology on efficacy and toxicity. Bioconjug Chem. 2006 Jan-Feb;17(1):114-24. [Content Brief]

[2]. Lee JW, et al. EphA2 targeted chemotherapy using an antibody drug conjugate in endometrial carcinoma. Clin Cancer Res. 2010 May 1;16(9):2562-70. [Content Brief]

[3]. Lee JJ, et al. Enzymatic prenylation and oxime ligation for the synthesis of stable and homogeneous protein-drug conjugates for targeted therapy. Angew Chem Int Ed Engl. 2015 Oct 5;54(41):12020-4. [Content Brief]

[4]. Kim EG, et al. Strategies and Advancement in Antibody-Drug Conjugate Optimization for Targeted CancerTherapeutics. [Content Brief]

MMAF Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass		Concentration		Volume		Molecular Weight *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)	×	Volume _(start)	=	Concentration _(final)	×	Volume _(final)
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MMAF (GMP)745017-94-1Monomethylauristatin F (GMP)Microtubule/TubulinADC PayloadADC CytotoxinInhibitorinhibitorinhibit

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