1. Apoptosis
  2. Apoptosis
  3. MN58b

MN58b 

Cat. No.: HY-108431 Purity: 99.72%
COA Handling Instructions

MN58b is a selective choline kinase α (CHKα) inhibitor, and results in inhibition of phosphocholine synthesis. MN58b reduces cell growth through the induction of apoptosis, and also has antitumoral activity.

For research use only. We do not sell to patients.

MN58b Chemical Structure

MN58b Chemical Structure

CAS No. : 203192-01-2

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1 mg USD 385 In-stock
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Based on 1 publication(s) in Google Scholar

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Description

MN58b is a selective choline kinase α (CHKα) inhibitor, and results in inhibition of phosphocholine synthesis. MN58b reduces cell growth through the induction of apoptosis, and also has antitumoral activity[1][2].

IC50 & Target

Choline kinase α (CHKα)[1]

In Vitro

The IC50s of MN58b for parental and Gemcitabine-resistant Suit2 007 cells are 3.14 μM and 0.77 μM, respectively[1].
? MN58b (1-5 μM; 72 hours; SK-PC-1, Suit2 008, IMIM-PC2, and RWP-1 cells) has a marked effect on colony formation at 1 μM, and growth is completely abolished at 5 μM in all the cell lines[1].
? MN58b ((1-10 μM; 24-48 hours; SK-PC-1, Suit2 008, IMIM-PC2, and RWP-1 cells) induces apoptosis and this response correlates with CHKα expression[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: SK-PC-1, Suit2 008, IMIM-PC2, and RWP-1 cells
Concentration: 1 µM, 5 µM
Incubation Time: 72 hours
Result: Inhibited cells growth.

Apoptosis Analysis[1]

Cell Line: SK-PC-1, Suit2 008, IMIM-PC2, and RWP-1 cells
Concentration: 1 µM, 2 µM, 5 µM, 10 µM
Incubation Time: 24 and 48 hours
Result: Induced cell apoptosis.
In Vivo

MN58b (4 mg/kg; intraperitoneal injection; once a day; for 5 days; MF-1 nude mice) treatment significantly decreases phosphomonoesters in both HT29 and MDA-MB-231 xenografts. Phosphocholine levels are found to correlate with choline kinase activities[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: MF-1 nude mice with HT29 or MDA-MB-231 cells[2]
Dosage: 4 mg/kg
Administration: Intraperitoneal injection; once a day; for 5 days
Result: Phosphomonoesters decreased significantly.
Molecular Weight

640.49

Formula

C32H40Br2N4

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CN(C)C1=CC=[N+](C=C1)CC2=CC=C(CCCCC3=CC=C(C[N+]4=CC=C(N(C)C)C=C4)C=C3)C=C2.[Br-].[Br-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 14.71 mg/mL (22.97 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5613 mL 7.8065 mL 15.6130 mL
5 mM 0.3123 mL 1.5613 mL 3.1226 mL
10 mM 0.1561 mL 0.7807 mL 1.5613 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.47 mg/mL (2.30 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 1.47 mg/mL (2.30 mM); Clear solution

*All of the co-solvents are available by MedChemExpress (MCE).
Purity & Documentation

Purity: 99.72%

References
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MN58b Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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MN58b
Cat. No.:
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