1. Apoptosis
  2. Apoptosis
  3. NAE-IN-M22

NAE-IN-M22 

Cat. No.: HY-115537 Purity: 99.67%
Handling Instructions

NAE-IN-M22 is a potent, selective and reversible inhibitor of NEDD8 activating enzyme (NAE), with potency in micromolar range. NAE-IN-M22 inhibits multiple cancer cell lines and induces apoptosis in A549 cells. NAE-IN-M22 also can inhibit tumor growth in vivo.

For research use only. We do not sell to patients.

NAE-IN-M22 Chemical Structure

NAE-IN-M22 Chemical Structure

CAS No. : 864420-54-2

Size Price Stock Quantity
5 mg USD 400 In-stock
Estimated Time of Arrival: December 31
10 mg USD 680 In-stock
Estimated Time of Arrival: December 31
25 mg USD 1350 In-stock
Estimated Time of Arrival: December 31
50 mg USD 2200 In-stock
Estimated Time of Arrival: December 31
100 mg USD 3400 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

NAE-IN-M22 is a potent, selective and reversible inhibitor of NEDD8 activating enzyme (NAE), with potency in micromolar range. NAE-IN-M22 inhibits multiple cancer cell lines and induces apoptosis in A549 cells. NAE-IN-M22 also can inhibit tumor growth in vivo[1].

IC50 & Target

NEDD8 activating enzyme (NAE)[1]

In Vitro

M22 (0.37-90 μM; 24 h) blocks neddylation pathway selectively and suppresses degradation of CRL substrates in A549 cells[1].
M22 (0.1-100 μM; 48 h) inhibits A549 cell proliferation completely at 30 µM (GI50=5.5 µM, GI90=19.3 µM)[1].
M22 (15-30 μM; 36 h) promotes apoptosis in A549 cell line[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: A549 cells
Concentration: 0.37, 1.11, 3.33, 10, 30, 90 μM
Incubation Time: 24 hours
Result: Inhibited formations of Uba3-NEDD8 and Ubc12-NEDD8.
Resulted in a corresponding decrease in the abundance of Cullins-NEDD8.
Decreased the degradations of p27 and CDT1.
Prevented p53 from degradation.
In Vivo

M22 (60 mg/kg; i.p. once daily for 14 d) inhibits tumor growth in nude mice bearing AGS xenografts[1].
M22 (0.36-90 μM) has low acute toxicity in zebrafish model[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

363.32

Formula

C₂₀H₂₄Cl₂N₂

CAS No.

864420-54-2

SMILES

ClC1=CC=C(CCNC2CCN(CC3=CC=CC=C3)CC2)C(Cl)=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (688.10 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7524 mL 13.7620 mL 27.5239 mL
5 mM 0.5505 mL 2.7524 mL 5.5048 mL
10 mM 0.2752 mL 1.3762 mL 2.7524 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (5.72 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (5.72 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (5.72 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Keywords:

NAE-IN-M22ApoptosisNEDD8activatingenzymeNAEcancerapoptosistumorInhibitorinhibitorinhibit

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Product Name:
NAE-IN-M22
Cat. No.:
HY-115537
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