Narirutin

Name: Narirutin
Brand: MedChemExpress
SKU: HY-N0804
Rating: 5.0 (5 reviews)

Cat. No.: HY-N0804 Purity: 99.95%: Data Sheet
COA

SDS
Handling Instructions
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Narirutin, one of the active constituents isolated from citrus fruits, has antioxidant and anti-inflammatory activities. Narirutin is a shikimate kinase inhibitor with anti-tubercular potency.

For research use only. We do not sell to patients.

CAS No. : 14259-46-2

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
20 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of Narirutin:

Isonaringin Get quote
Narirutin (Standard) Get quote

5 Publications Citing Use of MCE Narirutin

Bio/Physico-chemical Assay

RT-PCR

Histological Imaging/Staining

: Narirutin purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2023 Oct:166:115350. [Abstract]; Alanine transaminase (ALT) and aspartate transaminase (AST) activity in zebrafish larvae after alcoholic injury with or without Narirutin (NRT) (10, 40 μM) treatment.

: Narirutin purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2023 Oct:166:115350. [Abstract]; Real-time PCR analysis of the mRNA levels of inflammatory response genes treated with Narirutin (NRT) (40 μM).

: Narirutin purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2023 Oct:166:115350. [Abstract]; Oil-Red O staining was used to detect hepatic lipid accumulation in response to Narirutin (NRT) (10, 40 μM) treatment in zebrafish larvae.

: Narirutin purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2023 Oct:166:115350. [Abstract]; Protein expression levels of Ddit3, Bip and Gapdh. Quantification of band intensity of Ddit3 and Bip was calculated by Gadph as a housekeeping protein treated with Narirutin (NRT) (40 μM).

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Narirutin, one of the active constituents isolated from citrus fruits, has antioxidant and anti-inflammatory activities. Narirutin is a shikimate kinase inhibitor with anti-tubercular potency^[1]^[2].

Cellular Effect

Cell Line	Type	Value	Description	References
Monocyte	IC₅₀	> 200 μM Compound: Narirutin	Inhibition of TNFalpha expression in LPS-stimulated human monocytes treated 30 mins before LPS challenge measured after 14 hrs by ELISA Inhibition of TNFalpha expression in LPS-stimulated human monocytes treated 30 mins before LPS challenge measured after 14 hrs by ELISA	[PMID: 10096854]

In Vitro

Narirutin (200 μM, 20 h) has no effect on the vitality of RBL-2H3 cells^[3].
Narirutin (25 μM, 2.5 h) inhibits β-hex, histamine, IL-4 and TNF-a release were lower than 40% in RBL-2H3 cells. ^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Narirutin (10 mg/kg, p.o ) reduces eosinophil counts in peripheral blood in OVA-challenged mice^[2].
Narirutin (10 mg/kg, p.o ) block the OVA-induced increase in IL-4 but has no effect on the increase in IL-5 ^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Allergic asthma female NC/Nga mice model^[2]
Dosage:	0.1, 1 or 10 mg/kg
Administration:	Orally, 7d
Result:	Reduced eosinophil counts in peripheral blood. Blocked the OVA-induced increase in IL-4.

Clinical Trial

Molecular Weight

580.53

Formula

C₂₇H₃₂O₁₄

CAS No.

14259-46-2

Appearance

Solid

Color

White to light yellow

SMILES

O=C(C[C@@H](C1=CC=C(O)C=C1)OC2=CC(O[C@@H]([C@@H]([C@@H](O)[C@@H]3O)O)O[C@@H]3CO[C@H](O[C@@H](C)[C@H](O)[C@H]4O)[C@@H]4O)=C5)C2=C5O

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

H₂O : ≥ 100 mg/mL (172.26 mM)

DMSO : 100 mg/mL (172.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7226 mL	8.6128 mL	17.2256 mL
5 mM	0.3445 mL	1.7226 mL	3.4451 mL
10 mM	0.1723 mL	0.8613 mL	1.7226 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.08 mg/mL (3.58 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.08 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (3.58 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (3.58 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 10 mg/mL (17.23 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.95%

Select Batch:

Data Sheet (277 KB) SDS (394 KB)

COA (254 KB)

Handling Instructions (2659 KB)

References

[1]. Sahu PK, et al. Structure-based Discovery of Narirutin as a Shikimate Kinase Inhibitor with Anti-tubercular Potency.Curr Comput Aided Drug Des. 2019 Oct 25. [Content Brief]

[2]. Funaguchi N, et al. Narirutin inhibits airway inflammation in an allergic mouse model.Clin Exp Pharmacol Physiol. 2007 Aug;34(8):766-70. [Content Brief]

[3]. Liyan Niu, et al. Inhibitory activity of narirutin on RBL-2H3 cells degranulation. Immunopharmacol Immunotoxicol. 2021, 43, 1. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	1.7226 mL	8.6128 mL	17.2256 mL	43.0641 mL
	5 mM	0.3445 mL	1.7226 mL	3.4451 mL	8.6128 mL
	10 mM	0.1723 mL	0.8613 mL	1.7226 mL	4.3064 mL
	15 mM	0.1148 mL	0.5742 mL	1.1484 mL	2.8709 mL
	20 mM	0.0861 mL	0.4306 mL	0.8613 mL	2.1532 mL
	25 mM	0.0689 mL	0.3445 mL	0.6890 mL	1.7226 mL
	30 mM	0.0574 mL	0.2871 mL	0.5742 mL	1.4355 mL
	40 mM	0.0431 mL	0.2153 mL	0.4306 mL	1.0766 mL
	50 mM	0.0345 mL	0.1723 mL	0.3445 mL	0.8613 mL
	60 mM	0.0287 mL	0.1435 mL	0.2871 mL	0.7177 mL
	80 mM	0.0215 mL	0.1077 mL	0.2153 mL	0.5383 mL
	100 mM	0.0172 mL	0.0861 mL	0.1723 mL	0.4306 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Narirutin Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Narirutin14259-46-2BacterialInhibitorinhibitorinhibit

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Narirutin

Customer Review

Other Forms of Narirutin:

Narirutin Related Antibodies

5 Publications Citing Use of MCE Narirutin

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Narirutin Related Antibodies

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Dilution Calculator

Complete Stock Solution Preparation Table

Narirutin Related Classifications

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