1. GPCR/G Protein
  2. Orexin Receptor (OX Receptor)
  3. Orexin B, human TFA

Orexin B, human TFA (Synonyms: Human orexin B (TFA))

Cat. No.: HY-P1339A
Handling Instructions

Orexin B, human (TFA) is an endogenous agonist at Orexin receptor with Kis of 420 and 36 nM for OX1 and OX2, respectively.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Orexin B, human TFA Chemical Structure

Orexin B, human TFA Chemical Structure

Size Price Stock Quantity
10 mM * 1 mL in Water USD 7624 In-stock
Estimated Time of Arrival: December 31
500 μg USD 190 In-stock
Estimated Time of Arrival: December 31
1 mg USD 290 In-stock
Estimated Time of Arrival: December 31
5 mg USD 1150 In-stock
Estimated Time of Arrival: December 31
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50 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

Other Forms of Orexin B, human TFA:

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  • Biological Activity

  • Technical Information

  • Purity & Documentation

  • References

Description

Orexin B, human (TFA) is an endogenous agonist at Orexin receptor with Kis of 420 and 36 nM for OX1 and OX2, respectively.

IC50 & Target

Ki: 420 nM (OX1), 36 nM (OX2)[1].

In Vitro

Orexin B is derived by proteolytic amino acid precursor, prepro-orexin, which is encoded by a gene localized to chromosome 17q21 in humans. In radioligand binding studies, Orexin B has a higher affinity for the OX2 receptor[1].
Human Orexin B has two amino acid substitutions when compared with the rodent sequence. OX2 receptor is indeed a high-affinity receptor for human orexin B, with an IC50 of 36 nM in the binding assay and an EC50 of 60 nM in the [Ca2+]i transient assay. Human Orexin B has significantly lower affinity for the human OX1: the calculated IC50 in the competitive binding assay and the EC50 in the [Ca2+]i transient assay are 420 nM and 2500 nM for human orexin-B, respectively[2].

In Vivo

Human Orexin B significantly augments food intake; at the 2 hr time point, 5- and 12-fold stimulation of food consumption is observed by 3 nM and 30 nM orexin-B, respectively[2].

Animal Model: Male Wistar rats (180-200 g)[2].
Dosage: 3 nM and 30 nM.
Administration: Administered in a 5 mL bolus through a catheter placed in the left lateral ventricle in early light phase.
Result: Significantly augmented food intake.
Storage
Powder -80°C 2 years
  -20°C 1 year
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

  Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.  Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
References
Molecular Weight

3013.36

Formula

C₁₂₃H₂₁₂N₄₄O₃₅S.C₂HF₃O₂

Sequence

Arg-Ser-Gly-Pro-Pro-Gly-Leu-Gln-Gly-Arg-Leu-Gln-Arg-Leu-Leu-Gln-Ala-Ser-Gly-Asn-His-Ala-Ala-Gly-Ile-Leu-Thr-Met-NH2

Sequence Shortening

RSGPPGLQGRLQRLLQASGNHAAGILTM-NH2

Shipping

Room temperature in continental US; may vary elsewhere

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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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Product Name:
Orexin B, human TFA
Cat. No.:
HY-P1339A
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Orexin B, human TFA

Cat. No.: HY-P1339A