1. GPCR/G Protein
  2. Orexin Receptor (OX Receptor)

Orexin B, human (Synonyms: Human orexin B)

Cat. No.: HY-P1339
Handling Instructions

Orexin B, human is an endogenous agonist at Orexin receptor with Kis of 420 and 36 nM for OX1 and OX2, respectively. Sequence: Arg-Ser-Gly-Pro-Pro-Gly-Leu-Gln-Gly-Arg-Leu-Gln-Arg-Leu-Leu-Gln-Ala-Ser-Gly-Asn-His-Ala-Ala-Gly-Ile-Leu-Thr-Met-NH2;RSGPPGLQGRLQRLLQASGNHAAGILTM-NH2.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Orexin B, human Chemical Structure

Orexin B, human Chemical Structure

CAS No. : 205640-91-1

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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Orexin B, human is an endogenous agonist at Orexin receptor with Kis of 420 and 36 nM for OX1 and OX2, respectively. Sequence: Arg-Ser-Gly-Pro-Pro-Gly-Leu-Gln-Gly-Arg-Leu-Gln-Arg-Leu-Leu-Gln-Ala-Ser-Gly-Asn-His-Ala-Ala-Gly-Ile-Leu-Thr-Met-NH2;RSGPPGLQGRLQRLLQASGNHAAGILTM-NH2.

IC50 & Target

Ki: 420 nM (OX1), 36 nM (OX2)[1]

In Vitro

Orexin B is derived by proteolytic amino acid precursor, prepro-orexin, which is encoded by a gene localized to chromosome 17q21 in humans. In radioligand binding studies, Orexin B has a higher affinity for the OX2 receptor[1]. Human Orexin B has two amino acid substitutions when compared with the rodent sequence. OX2 receptor is indeed a high-affinity receptor for human orexin B, with an IC50 of 36 nM in the binding assay and an EC50 of 60 nM in the [Ca2+]i transient assay. Human Orexin B has significantly lower affinity for the human OX1: the calculated IC50 in the competitive binding assay and the EC50 in the [Ca2+]i transient assay are 420 nM and 2500 nM for human orexin-B, respectively[2].

In Vivo

Human Orexin B significantly augments food intake; at the 2 hr time point, 5- and 12-fold stimulation of food consumption is observed by 3 nM and 30 nM orexin-B, respectively[2].

Solvent & Solubility
In Vitro: 

10 mM in DMSO

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.3449 mL 1.7245 mL 3.4491 mL
5 mM 0.0690 mL 0.3449 mL 0.6898 mL
10 mM 0.0345 mL 0.1725 mL 0.3449 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Animal Administration
[2]

Rats[2]

3 nM and 30 nM of synthetic human Orexin B is administered in a 5 mL bolus through a catheter placed in the left lateral ventricle of Male Wistar rats (180–200 g) in early light phase. Cumulative food consumption is observed and plotted over the period of 4 hr after injection[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

2899.34

Formula

C₁₂₃H₂₁₂N₄₄O₃₅S

CAS No.

205640-91-1

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
Orexin B, human
Cat. No.:
HY-P1339
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Orexin B, human

Cat. No.: HY-P1339