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OSMI-2 

Cat. No.: HY-135784
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OSMI-2 (Compound 1b) is a cell-permeable O-linked N-acetylglucosamine transferase (OGT) inhibitor. Cells contain a large nuclear pool of partially spliced OGT transcript, and OSMI-2 increases detained intron splicing in cells.

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OSMI-2 Chemical Structure

OSMI-2 Chemical Structure

CAS No. : 2260542-60-5

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Description

OSMI-2 (Compound 1b) is a cell-permeable O-linked N-acetylglucosamine transferase (OGT) inhibitor. Cells contain a large nuclear pool of partially spliced OGT transcript, and OSMI-2 increases detained intron splicing in cells[1].

IC50 & Target

O-linked N-acetylglucosamine transferase (OGT)[1]

In Vitro

OSMI-2 (Compound 1b; 20-50 μM; 4-24 hours; HCT116 cells) treatment reduces O-GlcNAc levels at short treatment times (<8h). However, O-GlcNAc levels begins to recover at longer treatment times with OSMI-2[1]. A decrease in HCF-1 cleavage products and the appearance of uncleaved HCF-1 in cells treated with OSMI-2 (Compound 1b) is observed. Because OGT knockdown is known to decrease cell proliferation, the effects of OSMI-2 on cell growth in culture over 96h is also monitored. Although there is no evidence of apoptosis, the reduced growth of cells over time is observed, consistent with the knockdown results[1].
A time course performed with 20 μM OSMI-2 (Compound 1b) shows reciprocal changes in the abundance of OGT and OGA, with a particularly large increase in OGT; this may explain the recovery in O-GlcNAc levels with OSMI-2. At 24h where O-GlcNAc levels fully recovered[1].

Western Blot Analysis[1]

Cell Line: HCT116 cells
Concentration: 20 μM, 40 μM, 50 μM
Incubation Time: 4 hours, 24 hours
Result: Reduced O-GlcNAc levels.
Molecular Weight

555.62

Formula

C₂₆H₂₅N₃O₇S₂

CAS No.

2260542-60-5

SMILES

O=C(OC)CN(C([[email protected]@H](C1=CC=CC=C1OC)NS(=O)(C2=CC3=C(NC(C=C3)=O)C=C2)=O)=O)CC4=CC=CS4

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Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

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Keywords:

OSMI-2OSMI2OSMI 2OthersOGTestercell-permeableglycosyltransferasesO-GlcNAcnucleocytoplasmicglycosylationintronsplicingInhibitorinhibitorinhibit

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