PF-9363
Based on 5 publication(s) in Google Scholar
PF-9363 (CTx-648) is a first-in-class potent and high selective KAT6A/KAT6B inhibitor. PF-9363 can be used for the research of cancer.
For research use only. We do not sell to patients.
- Purity: 99.64%
- CAS No.: 2569009-58-9
- Formula: C20H20N4O6S
- Molecular Weight:444.46
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Epigenetics Compound Library Mini
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Epigenetics Compound Library
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Histone Modification Research Compound Library
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Anti-Cancer Compound Library
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Publications Citing Use of MedChemExpress (MCE) PF-9363
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Customer Validation & Images
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WB
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Flow Cytometry
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Cell Proliferation/Viability Assay
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Bio/Physico-chemical Assay
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Biological Activity
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MOZ/MORF |
PF-9363 (0~1 μM; 1 day; ZR75-1, T47D and MCF7 cells) down-regulates the expression of H3K23Ac biomarker[1].
PF-9363 leads to down regulation of a specific set of genes involved in ESR1 pathway, cell cycle and stem cell pathways. PF-9363 shows that the IC50 values for ZR75-1 and T47D are 0.3 nM and 0.9 nM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:ZR75-1, T47D and MCF7 cells
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Concentration:0~1 μM
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Incubation Time:1 day
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Result:Down-regulated the expression of H3K23Ac biomarker.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2569009-58-9
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Appearance Solid
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Molecular Weight 444.46
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Formula C20H20N4O6S
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Color White to off-white
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SMILES
O=S(C1=C(OC)C=CC=C1OC)(NC2=NOC3=C2C(OC)=CC(CN4N=CC=C4)=C3)=O
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Synonyms
CTx-648
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 1 year -20°C 6 months
Publications (5)
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Journal Impact Factor
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Most Recent
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Nat Cell Biol
2023 Sep;25(9):1346-1358. PMID: 37591951
PF-9363 purchased from MedChemExpress. Usage Cited in: Nat Cell Biol. 2023 Sep;25(9):1346-1358. [Abstract]
Immunoblot analysis of 1014 Kdm6a isogenic cells treated with inhibitors that block the function of epigenetic modifiers that normally maintain gene expression including VTP50469 (500 nM), EPZ-5676 (1 μM), PF-9363 (100 nM), JQAD1 or DMSO for 6 days.
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Am J Physiol Cell Physiol
2025 Jul 1;329(1):C38-C49. PMID: 40434042 -
bioRxiv
MYST acetyltransferases are a targetable therapeutic vulnerability in SETBP1-mutant leukemia. [Abstract]2026 Jan 8:2026.01.08.697228. PMID: 41542431 -
bioRxiv
KAT6A/B inhibition synergizes with retinoic acid and enhances the efficacy of GD2-targeted immunotherapy in neuroblastoma. [Abstract]2025 Jul 2:2025.07.01.662644. PMID: 40631259
PF-9363 purchased from MedChemExpress. Usage Cited in: bioRxiv. 2025 Jul 2:2025.07.01.662644. [Abstract]
Western blot analysis using antibodies to the indicated histones that were extracted from neuroblastoma cell line KELLY after treatment with the indicated doses of PF-9363 (0-10 μM) for 1, 3 and 6 days. Total H3 served as a loading control.
PF-9363 purchased from MedChemExpress. Usage Cited in: bioRxiv. 2025 Jul 2:2025.07.01.662644. [Abstract]
Flow cytometry showing GD2 expression levels in the GD2low cell lines BE2C, NGP and NB1 treated for 14 days with DMSO, isotretinoin alone, PF9363 (1 μM) alone, or isotretinoin plus PF-9363.
PF-9363 purchased from MedChemExpress. Usage Cited in: bioRxiv. 2025 Jul 2:2025.07.01.662644. [Abstract]
Cell viability of BE2C and NB1 cells following both treatment with either DMSO, isotretinoin alone, PF-9363 alone, or isotretinoin plus PF-9363 (1 μM) for 14 days and subsequent, co-culture with NK cells and dinutuximab for 24 hours.
PF-9363 purchased from MedChemExpress. Usage Cited in: bioRxiv. 2025 Jul 2:2025.07.01.662644. [Abstract]
Scatter plot of log2 fold-changes in H3K27me3 CUT&RUN signal at significant islands with lfc > 0.5 versus their fold-change rank. Experiments obtained from BE2C cells treated with PF-9363, isotretinoin or isotretinoin plus PF-9363 for 14 days at a concentration of 1 μM compared to control DMSO-treated BE2C cells. Significant islands associated with PHOX2B and GATA3 are noted.
PF-9363 purchased from MedChemExpress. Usage Cited in: bioRxiv. 2025 Jul 2:2025.07.01.662644. [Abstract]
Western-blot assay for transcription factors GATA3, PHOX2B and MYCN in BE2C cells treated with isotretinoin plus PF-9363 (1 μM) for 14 days followed by PF-9363 alone for 28 days.
Solvent & Solubility
DMSO : 50 mg/mL (112.50 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.68 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.08 mg/mL (4.68 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (278 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.2499 mL | 11.2496 mL | 22.4992 mL | 56.2480 mL |
| 5 mM | 0.4500 mL | 2.2499 mL | 4.4998 mL | 11.2496 mL | |
| 10 mM | 0.2250 mL | 1.1250 mL | 2.2499 mL | 5.6248 mL | |
| 15 mM | 0.1500 mL | 0.7500 mL | 1.4999 mL | 3.7499 mL | |
| 20 mM | 0.1125 mL | 0.5625 mL | 1.1250 mL | 2.8124 mL | |
| 25 mM | 0.0900 mL | 0.4500 mL | 0.9000 mL | 2.2499 mL | |
| 30 mM | 0.0750 mL | 0.3750 mL | 0.7500 mL | 1.8749 mL | |
| 40 mM | 0.0562 mL | 0.2812 mL | 0.5625 mL | 1.4062 mL | |
| 50 mM | 0.0450 mL | 0.2250 mL | 0.4500 mL | 1.1250 mL | |
| 60 mM | 0.0375 mL | 0.1875 mL | 0.3750 mL | 0.9375 mL | |
| 80 mM | 0.0281 mL | 0.1406 mL | 0.2812 mL | 0.7031 mL | |
| 100 mM | 0.0225 mL | 0.1125 mL | 0.2250 mL | 0.5625 mL |
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