2. PI3K/Akt/mTOR Apoptosis
  3. PI4K Apoptosis
  4. PI-273

PI-273 is a first reversibly and specific phosphatidylinositol 4-kinase (PI4KIIα) inhibitor with an IC50 of 0.47 μM. PI-273 can inhibit breast cancer cell proliferation, block the cell cycle and induce cell apoptosis.

For research use only. We do not sell to patients.

PI-273 Chemical Structure

PI-273 Chemical Structure

CAS No. : 925069-34-7

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 336 In-stock
Solution
10 mM * 1 mL in DMSO USD 336 In-stock
Solid
1 mg USD 80 In-stock
5 mg USD 160 In-stock
10 mg USD 270 In-stock
25 mg USD 550 In-stock
50 mg USD 900 In-stock
100 mg USD 1500 In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 6 publication(s) in Google Scholar

PI-273 Related Antibodies

Top Publications Citing Use of Products

    PI-273 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Oct 28;14(1):6883.  [Abstract]

    PI-273 (2 μM; 24, 48 h) causes a significant shift in PD-L1 distribution from Rab11-positive structures to LAMP1 lysosomes.

    PI-273 purchased from MedChemExpress. Usage Cited in: J Clin Invest. 2023 Feb 9;e165863.  [Abstract]

    PI-273 (25 mg/kg/day; i.p.; 2-4 weeks) inhibits growth of lung tumors in nu/nu mice.

    View All PI4K Isoform Specific Products:

    View All Isoforms
    PI4KIIIβ PI4KIIIα PI4K PI4KII

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    PI-273 is a first reversibly and specific phosphatidylinositol 4-kinase (PI4KIIα) inhibitor with an IC50 of 0.47 μM. PI-273 can inhibit breast cancer cell proliferation, block the cell cycle and induce cell apoptosis[1].

    IC50 & Target[1]

    PI4KIIα

    0.47 μM (IC50)

    In Vitro

    PI-273 (2 μM; 48 hours) blocks the cell cycle at the G2-M phase[1].
    PI-273 (2 μM; 48 hours) induces cell apoptosis in all three Ras wild-type breast cancer cells: MCF-7, T-47D, and SK-BR-3[1].
    PI-273 (0.5-2 μM; for 3 days) can suppress the AKT signaling pathway in a dose- and time-dependent manner[1].
    PI-273 of 1 μM and 2 μM inhibits the cell proliferation of both MCF-7 and T-47D cells in a time-dependent manner[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    PI-273 Related Antibodies

    Cell Cycle Analysis[1]

    Cell Line: MCF-7, T-47D, SK-BR-3, MDA-MB-231, SUM229PE, Hs 578T cells
    Concentration: 2 μM
    Incubation Time: 48 hours
    Result: Blocked the cell cycle at the G2-M phase.

    Apoptosis Analysis[1]

    Cell Line: MCF-7, T-47D, and SK-BR-3 cells
    Concentration: 2 μM
    Incubation Time: 48 hours
    Result: Induced cell apoptosis in all three Ras wild-type breast cancer cells: MCF-7, T-47D, and SK-BR-3.

    Western Blot Analysis[1]

    Cell Line: MCF-7 cells
    Concentration: 0.5, 1, 2 μM
    Incubation Time: For 3 days
    Result: Suppressed the AKT signaling pathway in a dose- and time-dependent manner.
    In Vivo

    PI-273 (intraperitoneal injection; 25 mg/kg/day; 15 days) profoundly suppresses the tumor volume and weight in the MCF-7 xenografts[1].
    PI-273 (0.5 mg/kg (intravenously) or 1.5 mg/kg (intragastrically); 0.08-5 hours) has a half-life of 0.411 hours for intravenous administration and 1.321 hours for intragastrical administration, and the absolute bioavailability of PI-273 is 5.1%[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Eight-week-old male BALB/c nude mice with MCF-7 cell[1]
    Dosage: 25 mg/kg
    Administration: Intraperitoneal injection; daily; 15 days
    Result: Suppressed the tumor volume and weight in the MCF-7 xenografts.
    Animal Model: Male Sprague-Dawley (SD) rats[1]
    Dosage: 0.5 mg/kg (intravenously) or 1.5 mg/kg (intragastrically) (Pharmacokinetic Study)
    Administration: Intravenously or intragastrically; 0.08, 0.16, 0.33, 0.67, 1, 1.5, 2, 3 and 5 hours
    Result: Has a half-life of 0.411 hours for intravenous administration and 1.321 hours for intragastrical administration, and the absolute bioavailability of PI-273 is 5.1%.
    Molecular Weight

    381.90

    Formula

    C16H16ClN3O2S2
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O=C(C1=C(NC(NC(C2=CC=C(Cl)C=C2)=O)=S)SC(C)=C1CC)N
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 6.02 mg/mL (15.76 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.6185 mL 13.0924 mL 26.1849 mL
    5 mM 0.5237 mL 2.6185 mL 5.2370 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: ≥98.0%

    COA (249 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.6185 mL 13.0924 mL 26.1849 mL 65.4622 mL
    5 mM 0.5237 mL 2.6185 mL 5.2370 mL 13.0924 mL
    10 mM 0.2618 mL 1.3092 mL 2.6185 mL 6.5462 mL
    15 mM 0.1746 mL 0.8728 mL 1.7457 mL 4.3641 mL
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    PI-273 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    PI-273925069-34-7PI273PI 273PI4KApoptosisPhosphatidylinositol 4 kinasesPI4 kinasesInhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Salutation

    Applicant Name *

    		 

    Email Address *

    Phone Number *

    		 

    Organization Name *

    Department *

    		 

    Requested quantity *

    Country or Region *

    		      

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    PI-273
    Cat. No.:
    HY-103489
    Quantity:
    MCE Japan Authorized Agent:

    Customer Review

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Lot #

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Country or Region *

    Remarks

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.