IFN-alpha/beta R2 Protein, Human (HEK293, His, solution)

IFN-α/β R2 protein forms a heterodimer with IFNAR1 and acts as a receptor for type I interferons. After interferon binding, the receptor subunit activates JAK, leading to the phosphorylation of STAT transcription factors. IFN-alpha/beta R2 Protein, Human (HEK293, His, solution) is the recombinant human-derived IFN-alpha/beta R2 protein, expressed by HEK293 , with C-His labeled tag.

The IFN-alpha/beta R2 protein, in conjunction with IFNAR1, constitutes the heterodimeric receptor responsible for recognizing type I interferons, including interferons alpha, beta, epsilon, omega, and kappa. Upon type I interferon binding, the IFNAR1 and IFNAR2 subunits are brought into close proximity, facilitating the activation of Janus kinases (JAKs) associated with each subunit (TYK2 bound to IFNAR1 and JAK1 bound to IFNAR2). This activation involves cross-phosphorylation of the JAKs, which, in turn, phosphorylate specific tyrosine residues on the intracellular domains of IFNAR1 and IFNAR2. These phosphorylated sites serve as docking sites for STAT transcription factors (STAT1, STAT2, and STAT3), initiating a signaling cascade that results in the translocation of STAT proteins into the nucleus. The translocated STAT proteins regulate the expression of interferon-regulated genes, contributing to the interferon response. Mechanistically, IFN-alpha/beta R2 acts as a potent inhibitor of type I IFN receptor activity, modulating the downstream effects of interferon signaling.

Measured by its ability to inhibit rh IFNβ mediated protection of WISH Human amnion cells infected with vesicular stomatitis virus (VSV) to viral lysis and the EC₅₀ is typically <4 μg/mL.

Human

HEK293

C-His

P48551-1 (I27-K243)

3455  [NCBI]

Extracellular Domain

ISYDSPDYTDESCTFKISLRNFRSILSWELKNHSIVPTHYTLLYTIMSKPEDLKVVKNCANTTRSFCDLTDEWRSTHEAYVTVLEGFSGNTTLFSCSHNFWLAIDMSFEPPEFEIVGFTNHINVMVKFPSIVEEELQFDLSLVIEEQSEGIVKKHKPEIKGNMSGNFTYIIDKLIPNTNYCVSVYLEHSDEQAVIKSPLKCTLLPPGQESESAESAK

Approximately 45 kDa

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.