S-Isopropylisothiourea hydrobromide (Synonyms: S-isopropyl ITU; IPTU)

Name: S-Isopropylisothiourea hydrobromide
Brand: MedChemExpress
SKU: HY-101304
Rating: 4.5 (1 reviews)

Cat. No.: HY-101304 Purity: 98.0%: Data Sheet
COA

SDS
Handling Instructions
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S-Isopropylisothiourea hydrobromide (S-isopropyl ITU; IPTU) is a blood-brain barrier (BBB) penetrant NOS inhibitor with IC₅₀ values of 0.66, 0.75 and 0.29 μM against mouse spinal cord, cerebellar, bovine aortic and porcine endothelial cell NOS. S-Isopropylisothiourea exhibits a significant blood pressure-raising effect without damaging the perfusion of vital organs and can also inhibit the late-phase pain response induced by formalin. S-Isopropylisothiourea hydrobromide is used in the researchs for hemorrhagic shock and pain response based on NOS.

For research use only. We do not sell to patients.

S-Isopropylisothiourea hydrobromide Chemical Structure

CAS No. : 4269-97-0

Size	Stock	Quantity
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	Get quote
200 mg	Get quote

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Based on 1 publication(s) in Google Scholar

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nNOS iNOS eNOS

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Description

IC₅₀ & Target^[1]^[2]

eNOS

0.5 μM (IC₅₀)

nNOS

0.66 μM (IC₅₀)

nNOS

0.75 μM (IC₅₀)

eNOS

0.29 μM (IC₅₀)

In Vivo

S-Isopropylisothiourea hydrobromide (50 mg/kg, i.p., single dose) can pass through the blood-brain barrier and reach an effective concentration in the central nervous system, and achieves a potent and sustained blood-pressure-raising effect by inhibiting vascular eNOS in mice^[1].
S-Isopropylisothiourea hydrobromide (10-50 mg/kg, i.p., single dose) exhibits selective analgesic effects at a dose of 50 mg/kg, specifically inhibiting pain in the late phase (15-30 min)^[1].
S-Isopropylisothiourea hydrobromide (0.1 mg/kg, i.v., 3 doses) significantly increases mean arterial pressure (MAP) and achieve a 100% survival rate in a rat model of severe hemorrhagic shock^[2].
S-Isopropylisothiourea hydrobromide (0.3 mg/kg plus 1 mg/kg·h, i.v., maintain infusion for 120 min) significantly increases MAP to a normal level, without reducing cardiac output, and maintain perfusion of vital organs^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Formalin-induced hindpaw licking behaviour established in mice (male, LACA, 25-35 g)^[1]
Dosage:	10, 20 and 30 mg/kg
Administration:	Intraperitoneal injection (i.p.), single dose
Result:	Reduced the movement and grooming behaviors of mice. Did not affect the paw edema induced by formalin.

Animal Model:	Pylorus-ligated model established in male Wistar rats^[2]
Dosage:	0.1 mg/kg
Administration:	Intravenous injection (i.v.), 10 min, 20 min, and 40 min, administered in three doses
Result:	Increased MAP to 65-70 mmHg, and the survival rate was 100%.

Animal Model:	Porcine hemorrhagic shock model established in York shire pigs (6-10 weeks, 10-15.5 kg)^[2]
Dosage:	0.3 mg/kg plus 1 mg/kg·h
Administration:	Intravenous injection (i.v.), maintain infusion for 120 min
Result:	Increased MAP to 90 mmHg. Maintained a stable heart rate and do not affect the cardiac output. Increased the vascular resistance of the system.

Molecular Weight

199.11

Formula

C₄H₁₁BrN₂S

CAS No.

4269-97-0

Appearance

Solid

Color

White to off-white

SMILES

NC(SC(C)C)=N.Br

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (251.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.0223 mL	25.1117 mL	50.2235 mL
5 mM	1.0045 mL	5.0223 mL	10.0447 mL
10 mM	0.5022 mL	2.5112 mL	5.0223 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (12.56 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (12.56 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (12.56 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.0%

Select Batch:

Data Sheet (274 KB) SDS (393 KB)

COA (245 KB)

Handling Instructions (2659 KB)

References

[1]. Handy RL, Wallace P, Moore PK. Inhibition of nitric oxide synthase by isothioureas: cardiovascular and antinociceptive effects. Pharmacol Biochem Behav. 1996 Oct;55(2):179-84. [Content Brief]

[2]. A Vromen, et al. Effects of S-isopropyl isothiourea, a potent inhibitor of nitric oxide synthase, in severe hemorrhagic shock. J Appl Physiol (1985). 1996 Aug;81(2):707-15. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.0223 mL	25.1117 mL	50.2235 mL	125.5587 mL
	5 mM	1.0045 mL	5.0223 mL	10.0447 mL	25.1117 mL
	10 mM	0.5022 mL	2.5112 mL	5.0223 mL	12.5559 mL
	15 mM	0.3348 mL	1.6741 mL	3.3482 mL	8.3706 mL
	20 mM	0.2511 mL	1.2556 mL	2.5112 mL	6.2779 mL
	25 mM	0.2009 mL	1.0045 mL	2.0089 mL	5.0223 mL
	30 mM	0.1674 mL	0.8371 mL	1.6741 mL	4.1853 mL
	40 mM	0.1256 mL	0.6278 mL	1.2556 mL	3.1390 mL
	50 mM	0.1004 mL	0.5022 mL	1.0045 mL	2.5112 mL
	60 mM	0.0837 mL	0.4185 mL	0.8371 mL	2.0926 mL
	80 mM	0.0628 mL	0.3139 mL	0.6278 mL	1.5695 mL
	100 mM	0.0502 mL	0.2511 mL	0.5022 mL	1.2556 mL

S-Isopropylisothiourea hydrobromide Related Classifications

Neurological Disease Cardiovascular Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

S-Isopropylisothiourea4269-97-0S-isopropyl ITU IPTUNO SynthaseNitric oxide synthasesNOSIsothioureaMethylisothioureaIsopropylisothioureaNitric oxide synthaseBlood pressureAntinociceptionInhibitorinhibitorinhibit

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S-Isopropylisothiourea hydrobromide (Synonyms: S-isopropyl ITU; IPTU)

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S-Isopropylisothiourea hydrobromide Related Antibodies

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Complete Stock Solution Preparation Table

S-Isopropylisothiourea hydrobromide Related Classifications

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