2. Cell Cycle/DNA Damage TGF-beta/Smad Stem Cell/Wnt Cytoskeleton
  3. ROCK
  4. Scaff10-8

Scaff10-8 is a RhoA inhibitor. Scaff10-8 binds to RhoA, inhibits AKAP-Lbc-mediated RhoA activation. Scaff10-8 can be used for research on diabetes insipidus and bipolar disorder.

For research use only. We do not sell to patients.

Scaff10-8

Scaff10-8 Chemical Structure

CAS No. : 777857-56-4

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Based on 1 publication(s) in Google Scholar

Scaff10-8 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Scaff10-8

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  • Biological Activity

  • Purity & Documentation

  • References

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Description

Scaff10-8 is a RhoA inhibitor. Scaff10-8 binds to RhoA, inhibits AKAP-Lbc-mediated RhoA activation. Scaff10-8 can be used for research on diabetes insipidus and bipolar disorder[1].

In Vitro

Scaff10-8 (1 h) potently inhibits the interaction between recombinant AKAP-Lbc/DHPH and RhoA in an HTRF assay with an IC50 of 34.1 μM[1].
Scaff10-8 inhibits the interaction between recombinant AKAP-Lbc DH domain and RhoA in an Alpha Screen assay with an IC50 of 42 μM[1].
Scaff10-8 (1 h) binds directly to recombinant 647-RhoA with a Kd of 20 μM, as measured by MST[1].
Scaff10-8 binds specifically to both GDP-bound and GTPγS-bound recombinant GST-RhoA, with Kd values of 20 μM and 38 μM, respectively[1].
Scaff10-8 (30-100 μM; 24-72 h) is not cytotoxic to primary IMCD, MCD4, HEK293, MCF7, or H9C2 cells[1].
Scaff10-8 (30 μM; 1 h) inhibits RhoA activity in primary IMCD cells[1].
Scaff10-8 (30 μM; 1 h) does not affect the activity of Cdc42 or Rac1 in primary IMCD cells[1].
Scaff10-8 (30 μM; 1 h) promotes the redistribution of AQP2 to the periphery of primary IMCD cells and depolymerizes F-actin[1].
Scaff10-8 (30 μM; 1-24 h) does not promote the insertion of AQP2 into the plasma membrane of primary IMCD cells[1].
Scaff10-8 (30-100 μM; 24 h) selectively inhibits AKAP-Lbc-mediated RhoA activation in HEK293 cells, with no effect on LARG or PDZ-RhoGEF-mediated RhoA activation, when treated at 30 or 100 μM[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Scaff10-8 Related Antibodies

Cell Viability Assay[1]

Cell Line: primary rat inner medullary collecting duct (IMCD) cells, mouse collecting duct MCD4 cells, human embryonic kidney HEK293 cells, breast carcinoma MCF7 cells, cardiac myocyte H9C2 cells
Concentration: 30 μM; 100 μM
Incubation Time: 24 h; 48 h; 72 h
Result: Did not reduce cell viability in any of the tested cell lines across all concentrations and incubation times, as indicated by absorbance values comparable to untreated and DMSO control cells.

Immunofluorescence[1]

Cell Line: primary rat IMCD cells
Concentration: 30 μM
Incubation Time: 1 h
Result: Induced redistribution of AQP2 to the cell periphery, with a plasma membrane/perinuclear AQP2 ratio significantly higher than control cells.
Caused depolymerization of F-actin, with fiber structures replaced by dot-like fragments.
Molecular Weight

378.37

Formula

C22H18O6
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(CCC1=C(C2=CC3=C(C=C2OC1=O)OC=C3C4=CC=C(C=C4)OC)C)O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (264.29 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6429 mL 13.2146 mL 26.4292 mL
5 mM 0.5286 mL 2.6429 mL 5.2858 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.6429 mL 13.2146 mL 26.4292 mL 66.0729 mL
5 mM 0.5286 mL 2.6429 mL 5.2858 mL 13.2146 mL
10 mM 0.2643 mL 1.3215 mL 2.6429 mL 6.6073 mL
15 mM 0.1762 mL 0.8810 mL 1.7619 mL 4.4049 mL
20 mM 0.1321 mL 0.6607 mL 1.3215 mL 3.3036 mL
25 mM 0.1057 mL 0.5286 mL 1.0572 mL 2.6429 mL
30 mM 0.0881 mL 0.4405 mL 0.8810 mL 2.2024 mL
40 mM 0.0661 mL 0.3304 mL 0.6607 mL 1.6518 mL
50 mM 0.0529 mL 0.2643 mL 0.5286 mL 1.3215 mL
60 mM 0.0440 mL 0.2202 mL 0.4405 mL 1.1012 mL
80 mM 0.0330 mL 0.1652 mL 0.3304 mL 0.8259 mL
100 mM 0.0264 mL 0.1321 mL 0.2643 mL 0.6607 mL
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Scaff10-8 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Scaff10-8777857-56-4ROCKRho-associated protein kinaseRho-associated kinaseRho-kinaseROKRhoARac1Alpha Screen assayHEK293HTRF assayCdc42aquaporin-2primary renal inner medullary collecting duct cellsAKAP-LbcMCF7Inhibitorinhibitorinhibit

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