1. MAPK/ERK Pathway Apoptosis
  2. p38 MAPK Apoptosis
  3. SSK1

SSK1 

Cat. No.: HY-138936 Purity: 99.19%
COA Handling Instructions

SSK1, a senescence-specific killing compound, is a β-galactosidase-targeted proagent attenuates inflammation. SSK1 is activated by lysosomal β-galactosidase and selectively killed senescent cells through the activation of p38 MAPK and induction of apoptosis.

For research use only. We do not sell to patients.

SSK1 Chemical Structure

SSK1 Chemical Structure

CAS No. : 2629250-69-5

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5 mg USD 650 In-stock
10 mg USD 1050 In-stock
25 mg USD 2100 In-stock
50 mg USD 3360 In-stock
100 mg USD 5200 In-stock
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This product is a controlled substance and not for sale in your territory.

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Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products

1 Publications Citing Use of MCE SSK1

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Description

SSK1, a senescence-specific killing compound, is a β-galactosidase-targeted proagent attenuates inflammation. SSK1 is activated by lysosomal β-galactosidase and selectively killed senescent cells through the activation of p38 MAPK and induction of apoptosis[1].

In Vitro

SSK1 (0.5 µM; 12-72 hours) activates the phosphorylation levels of both p38 MAPK and MKK3/MKK6 in senescent cells. SSK1 kills senescent cells through the activation of the p38 MAPK signaling pathway. SSK1 is able to induce mitochondrial DNA damage in senescent cells[1].
SSK1 (0.01-1 µM; 3 days) selectively and potently eliminates β-galactosidase-positive senescent cells within a wide therapeutic window[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay[1]

Cell Line: Primary mouse fibroblasts
Concentration: 0.5 µM
Incubation Time: 12 hours, 24 hours, 36 hours, 48 hours, 72 hours
Result: Both p38 MAPK and MKK3/MKK6 were activated by phosphorylation in senescent cells.
In Vivo

SSK1 (0.5 mg/kg; i.p.; two days every week; for four weeks) could eliminate senescent cells and decrease senescence-associated markers in lung-injured mice[1].
In aged mice (20-month-old), SSK1 (0.5 mg/kg; 3 days every 2 weeks for 8 weeks) effectively clears senescent cells in different tissues, decreases the senescence- and age-associated gene signatures, attenuates low-grade local and systemic inflammation, and restores physical function[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Mice (3-6-month-old) were subjected to transtracheal injection of Bleomycin[1]
Dosage: 0.5 mg/kg
Administration: Intraperitoneally injection; two days every week; for four weeks
Result: SSK1 significantly reduced the percentage of SA-β-gal-positive cells in lung by 3.8-fold compared with that in vehicle-treated lung-injured mice
Molecular Weight

788.61

Formula

C31H34F2N4O18

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC(O[C@H]([C@H]([C@H]1OC(C)=O)OC(C)=O)[C@@H](O[C@@H]1COC(C)=O)OC(C=CC(COC(NC(C=CN2[C@](C(F)([C@@H]3O)F)([H])O[C@@H]3CO)=NC2=O)=O)=C4)=C4[N+]([O-])=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 200 mg/mL (253.61 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2681 mL 6.3403 mL 12.6805 mL
5 mM 0.2536 mL 1.2681 mL 2.5361 mL
10 mM 0.1268 mL 0.6340 mL 1.2681 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 5 mg/mL (6.34 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 5 mg/mL (6.34 mM); Clear solution

*All of the co-solvents are available by MedChemExpress (MCE).
Purity & Documentation

Purity: 99.19%

References
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SSK1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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SSK1
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