Surfen dihydrochloride
Based on 2 publication(s) in Google Scholar
Surfen dihydrochloride is a potent heparan sulfate antagonist. Surfen inhibits FGF2 binding and signal transduction. Surfen binds to glycosaminoglycans and reduces tau hyperphosphorylation. Surfen dihydrochloride inhibits the activity of recombinant uronyl 2-O-sulfotransferase with an IC50 of approximately 2 μM. Surfen dihydrochloride inhibits HSV-1 viral infection. Surfen dihydrochloride inhibits neural differentiation, delays remyelination, and alleviates EAE.
For research use only. We do not sell to patients.
- Purity: 99.93%
- CAS No.: 5424-37-3
- Formula: C21H22Cl2N6O
- Molecular Weight:445.34
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Surfen dihydrochloride
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Biological Activity
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FGFR2 5 μM (IC50) |
HSV-1 |
Surfen (3 μM) dihydrochloride binds to glycosaminoglycans (GAGs)[1].
Surfen (1 mg) dihydrochloride neutralizes the anticoagulant activity of both unfractionated and low molecular weight heparins[1].
Surfen dihydrochloride inhibits the activity of recombinant uronyl 2-O-sulfotransferase, with an IC50 of ~2 μM[1].
Surfen (1-≥5 μM) dihydrochloride inhibits cell attachment in a dose-dependent manner, and completely inhibits HSV-1 infection in CHO cells[1].
Surfen (5 μM) dihydrochloride effectively inhibits neural differentiation and promotes the maintenance of pluripotency in mouse embryonic stem cells (mESCs)[2].
Surfen (2.5-20 μM) dihydrochloride reduces the proliferation of murine T cells activated with anti-CD3/CD28 antibody-coated T cell expander beads[6].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Surfen (5 mg/kg; i.p.; every other day) dihydrochloride alleviates clinical symptoms and reduces the infiltration of CD4 positive T cells and macrophages into the central nervous system in the experimental autoimmune encephalomyelitis (EAE) model of multiple sclerosis in mice[5].
Surfen (100 μM; injection into the right burr hole) dihydrochloride delays remyelination of the lesions in the Lysolecithin (LPC)-induced demyelination mouse model[5].
Surfen (20 mg/kg; i.p.; daily; 3 consecutive days) dihydrochloride inhibits the proliferation of T cells activated by anti-CD3 antibody in mice[6].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6 adult female mice (16-20 g, 6-8 weeks of age), experimental autoimmune encephalomyelitis (EAE) model[5]
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Dosage:5 mg/kg
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Administration:Intraperitoneal injection, every other day
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Result:Significantly reduced clinical scores between days 13-21.
Reduced mean clinical score to 1.96.
Significantly reduced the number of days with a score of 2.5 or above.
Reduced the percentage of CD4 positive T cells and macrophages in the spinal cord and cerebellum, and decreased the mRNA expression of chemokines CCL3 and CCL5 in the spinal cord.
Chemical Information
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CAS No. 5424-37-3
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Appearance Solid
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Molecular Weight 445.34
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Formula C21H22Cl2N6O
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Color Light brown to gray
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SMILES
O=C(NC1=CC=C2N=C(C)C=C(N)C2=C1)NC3=CC=C4N=C(C)C=C(N)C4=C3.Cl.Cl
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Synonyms
Aminoquinuride dihydrochloride
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (2)
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Journal Impact Factor
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Most Recent
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Microbiol Spectr
Aminoquinoline surfen inhibits pseudorabies virus attachment by preventing the binding of glycoprotein C to heparan sulfate. [Abstract]2026 Apr 7;14(4):e0322325. PMID: 41784488 -
Solvent & Solubility
DMSO : 50 mg/mL (112.27 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 8 mg/mL (17.96 mM; Need ultrasonic and warming)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (279 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Schuksz M, et al. Surfen, a small molecule antagonist of heparan sulfate. Proc Natl Acad Sci U S A. 2008 Sep 2;105(35):13075-80. [Content Brief]
[2]. Huang ML, et al. Small Molecule Antagonist of Cell Surface Glycosaminoglycans Restricts Mouse Embryonic Stem Cells in a Pluripotent State. Stem Cells. 2018 Jan;36(1):45-54. [Content Brief]
[3]. Varatharajan A, et al. Visual determination of heparin in serum utilizing surfen-induced aggregation emission enhancement of gold nanoclusters and heparin-induced fluorescence enhancement of surfen. Spectrochim Acta A Mol Biomol Spectrosc. 2025 Apr 15;339:126251. [Content Brief]
[4]. Alavi Naini SM, et al. Surfen and oxalyl surfen decrease tau hyperphosphorylation and mitigate neuron deficits in vivo in a zebrafish model of tauopathy. Transl Neurodegener. 2018 Mar 16;7:6. [Content Brief]
[5]. Warford JR, et al. Surfen, a proteoglycan binding agent, reduces inflammation but inhibits remyelination in murine models of Multiple Sclerosis. Acta Neuropathol Commun. 2018 Jan 4;6(1):4. [Content Brief]
[6]. Warford J, et al. Murine T cell activation is regulated by surfen (bis-2-methyl-4-amino-quinolyl-6-carbamide). Biochem Biophys Res Commun. 2014 Jan 10;443(2):524-30. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 2.2455 mL | 11.2274 mL | 22.4548 mL | 56.1369 mL |
| 5 mM | 0.4491 mL | 2.2455 mL | 4.4910 mL | 11.2274 mL | |
| 10 mM | 0.2245 mL | 1.1227 mL | 2.2455 mL | 5.6137 mL | |
| 15 mM | 0.1497 mL | 0.7485 mL | 1.4970 mL | 3.7425 mL | |
| DMSO | 20 mM | 0.1123 mL | 0.5614 mL | 1.1227 mL | 2.8068 mL |
| 25 mM | 0.0898 mL | 0.4491 mL | 0.8982 mL | 2.2455 mL | |
| 30 mM | 0.0748 mL | 0.3742 mL | 0.7485 mL | 1.8712 mL | |
| 40 mM | 0.0561 mL | 0.2807 mL | 0.5614 mL | 1.4034 mL | |
| 50 mM | 0.0449 mL | 0.2245 mL | 0.4491 mL | 1.1227 mL | |
| 60 mM | 0.0374 mL | 0.1871 mL | 0.3742 mL | 0.9356 mL | |
| 80 mM | 0.0281 mL | 0.1403 mL | 0.2807 mL | 0.7017 mL | |
| 100 mM | 0.0225 mL | 0.1123 mL | 0.2245 mL | 0.5614 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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