2'-O-MOE-U
Based on 1 Customer Validation
2'-O-MOE-U is a nucleic acid modification group (Phosphoramidite) with 3'-exonuclease inhibitory activity. 2'-O-MOE-U also exhibits gene silencing activity and double-stranded oligonucleotide stability. By forming steric interactions with 3'-exonuclease residues, 2'-O-MOE-U anchors the 3'-end of the siRNA guide strand in the hAgo2 PAZ domain, thereby regulating double-stranded thermal stability and enhancing base-pairing specificity. 2'-O-MOE-U does not induce IFNα production, can be incorporated at multiple sites of siRNA to enhance RNAi activity, and produces a synergistic effect with 2'-F modification. 2'-O-MOE-U has been widely used in studies related to breast cancer and other diseases.
For research use only. We do not sell to patients.
- Purity: 95.0%
- CAS No.: 163759-97-5
- Formula: C42H53N4O10P
- Molecular Weight:804.86
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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All DNA/RNA Synthesis Isoforms
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Biological Activity
2′-O-MOE-U (35 mU/mL SVPD; up to 180 min) modification increases the stability of dT10-mer oligonucleotides against 3′-exonuclease (SVPD), with ON7 exhibiting a T1/2 of ~22 min[1].
2'-O-MOE-U modification increases the serum stability of 14-mer single-stranded DNA, with a half-life of 206.9 min in 10% FBS, compared to 33.4 min for unmodified DNA[2].
2'-O-MOE-U (0.5 nM) reduces off-target effects while exhibiting approximately 60% targeted gene silencing at 0.5 nM in lipofected cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 163759-97-5
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Appearance Solid
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Molecular Weight 804.86
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Formula C42H53N4O10P
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Color White to off-white
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SMILES
COC(C=C1)=CC=C1C(C2=CC=C(OC)C=C2)(C3=CC=CC=C3)OC[C@H]4O[C@@H](N5C(NC(C=C5)=O)=O)[C@H](OCCOC)[C@@H]4OP(N(C(C)C)C(C)C)OCCC#N
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : ≥ 50 mg/mL (62.12 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (280 KB)
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SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
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Handling Instructions (2659 KB)
References
[1]. Gangopadhyay S, et al. 4'-O-methoxyethyl-2'-deoxy-uridine and cytidine ribonucleotides improve duplex stability, nuclease resistance, and elicit efficient knockdown of Bcl-2 expression. Bioorg Med Chem Lett. 2025;128:130329. [Content Brief]
[2]. Gangopadhyay S, et al. 4'-C-Acetamidomethyl-2'-O-methoxyethyl Nucleic Acid Modifications Improve Thermal Stability, Nuclease Resistance, Potency, and hAgo2 Binding of Small Interfering RNAs. J Org Chem. 2024;89(6):3747-3768. [Content Brief]
[3]. Lyu F, et al. A 2'-modified uridine analog, 2'-O-(methylthiomethoxy)methyl uridine, for siRNA applications. Bioorg Med Chem Lett. 2022;74:128939. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.2425 mL | 6.2123 mL | 12.4245 mL | 31.0613 mL |
| 5 mM | 0.2485 mL | 1.2425 mL | 2.4849 mL | 6.2123 mL | |
| 10 mM | 0.1242 mL | 0.6212 mL | 1.2425 mL | 3.1061 mL | |
| 15 mM | 0.0828 mL | 0.4142 mL | 0.8283 mL | 2.0708 mL | |
| 20 mM | 0.0621 mL | 0.3106 mL | 0.6212 mL | 1.5531 mL | |
| 25 mM | 0.0497 mL | 0.2485 mL | 0.4970 mL | 1.2425 mL | |
| 30 mM | 0.0414 mL | 0.2071 mL | 0.4142 mL | 1.0354 mL | |
| 40 mM | 0.0311 mL | 0.1553 mL | 0.3106 mL | 0.7765 mL | |
| 50 mM | 0.0248 mL | 0.1242 mL | 0.2485 mL | 0.6212 mL | |
| 60 mM | 0.0207 mL | 0.1035 mL | 0.2071 mL | 0.5177 mL |
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