2. Metabolic Enzyme/Protease Apoptosis
  3. E1/E2/E3 Enzyme Apoptosis
  4. VII-31

VII-31 is a potent NEDDylation pathway activator to inhibit the tumor progression in vitro and in vivo. VII-31 induces apoptosis via intrinsic and extrinsic pathways.

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VII-31 Chemical Structure

VII-31 Chemical Structure

CAS No. : 2305757-96-2

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Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 330 In-stock
Solution
10 mM * 1 mL in DMSO USD 330 In-stock
Solid
5 mg USD 300 In-stock
10 mg USD 480 In-stock
25 mg USD 900 In-stock
50 mg USD 1350 In-stock
100 mg USD 2000 In-stock
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Based on 1 publication(s) in Google Scholar

VII-31 Related Antibodies

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Description

VII-31 is a potent NEDDylation pathway activator to inhibit the tumor progression in vitro and in vivo. VII-31 induces apoptosis via intrinsic and extrinsic pathways[1].

IC50 & Target

NEDDylation[1]
In Vitro

VII-31 (100 nM, 200 nM; 48 hours) inhibits the cell viability of gastric cell line MGC803 with an IC50 of 0.09±0.01 μM. VII-31 also inhibits the cell viability of MCF-7 and PC-3 with IC50s of 0.10±0.006 and 1.15±0.28μM , respectively[1].
VII-31 (50-150 nM; 24 hours) arrests MGC803 cells cycle in G2/M phase[1].
VII-31 (50-150 nM; 48 hours) induces apoptosis via intrinsic and extrinsic pathways[1].
VII-31 (50-150 nM; 24 hours) activates NEDDylation in MGC803 cells[1].
VII-31 (50-150 nM; 48 hours) up-regulates pro-apoptotic proteins FADD, Fasl, PIDD, Bax, Bad; while down-regulates anti-apoptotic proteins Bcl-xL, Bcl-2, XIAP, c-IAP1[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

VII-31 Related Antibodies

Cell Viability Assay[1]

Cell Line: Gastric cancer MGC803 cells
Concentration: 100, 200 nM
Incubation Time: 48 hours
Result: Inhibited the cell viability in dose-depend manner.

Cell Cycle Analysis[1]

Cell Line: MGC803 cells
Concentration: 50, 100, 150 nM
Incubation Time: 24 hours
Result: Arrested cells in G2/M phase, and a clear sub-G1 peak was observed in the high dose group.

Apoptosis Analysis[1]

Cell Line: MGC803 cells
Concentration: 50, 75, 100, and 150 nM
Incubation Time: 48 hours
Result: High dose (150 nM) treatment significantly elevated the early and late apoptosis rate to 92.8% from 4.8%.

Western Blot Analysis[1]

Cell Line: MGC803 cells
Concentration: 50, 100, 150 nM
Incubation Time: 24 hours
Result: Resulted in NEDDylation activation of MGC803 cells, the NEDDylation of 3 important proteins NAE1, Ubc12 and CUL1 has been activated.
In Vivo

VII-31 inhibits the tumor progression in vivo, while showing no obvious toxicity to mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Mice bearing MGC803 xenograft tumors[1]
Dosage: 50, 100, 150 mg/kg
Administration: Subcutaneous injection; daily for 28 days
Result: The mice had a much smaller tumor compared with vehicle control. The tumor volumes of middle/high dose treated mice at certain time points were evidently decreased comparing with untreated group.
Molecular Weight

427.51

Formula

C23H25NO5S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(CC1=CC=CS1)N(CC2=CC=C(OC)C=C2)C3=CC(OC)=C(OC)C(OC)=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (584.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3391 mL 11.6956 mL 23.3913 mL
5 mM 0.4678 mL 2.3391 mL 4.6783 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (4.87 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.08 mg/mL (4.87 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.3391 mL 11.6956 mL 23.3913 mL 58.4782 mL
5 mM 0.4678 mL 2.3391 mL 4.6783 mL 11.6956 mL
10 mM 0.2339 mL 1.1696 mL 2.3391 mL 5.8478 mL
15 mM 0.1559 mL 0.7797 mL 1.5594 mL 3.8985 mL
20 mM 0.1170 mL 0.5848 mL 1.1696 mL 2.9239 mL
25 mM 0.0936 mL 0.4678 mL 0.9357 mL 2.3391 mL
30 mM 0.0780 mL 0.3899 mL 0.7797 mL 1.9493 mL
40 mM 0.0585 mL 0.2924 mL 0.5848 mL 1.4620 mL
50 mM 0.0468 mL 0.2339 mL 0.4678 mL 1.1696 mL
60 mM 0.0390 mL 0.1949 mL 0.3899 mL 0.9746 mL
80 mM 0.0292 mL 0.1462 mL 0.2924 mL 0.7310 mL
100 mM 0.0234 mL 0.1170 mL 0.2339 mL 0.5848 mL
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VII-31 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

VII-312305757-96-2VII31VII 31E1/E2/E3 EnzymeApoptosisE1 activating enzymeE2 conjugating enzymeE3 ligating enzymeUbiquitin activating enzymeUbiquitin conjugating enzymeUbiquitin ligaseNAE1Ubc12Cullin1NEDDylationIAPMGC803MCF-7PC-3Inhibitorinhibitorinhibit

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