1. Metabolic Enzyme/Protease
    Apoptosis
  2. E1/E2/E3 Enzyme
    Apoptosis
  3. VII-31

VII-31 

Cat. No.: HY-133558
Handling Instructions

VII-31 is a potent NEDDylation pathway activator to inhibit the tumor progression in vitro and in vivo. VII-31 induces apoptosis via intrinsic and extrinsic pathways.

For research use only. We do not sell to patients.

VII-31 Chemical Structure

VII-31 Chemical Structure

CAS No. : 2305757-96-2

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10 mM * 1  mL in DMSO USD 330 In-stock
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Based on 1 publication(s) in Google Scholar

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Description

VII-31 is a potent NEDDylation pathway activator to inhibit the tumor progression in vitro and in vivo. VII-31 induces apoptosis via intrinsic and extrinsic pathways[1].

IC50 & Target

NEDDylation[1]

In Vitro

VII-31 (100 nM, 200 nM; 48 hours) inhibits the cell viability of gastric cell line MGC803 with an IC50 of 0.09±0.01 μM. VII-31 also inhibits the cell viability of MCF-7 and PC-3 with IC50s of 0.10±0.006 and 1.15±0.28μM , respectively[1].
VII-31 (50-150 nM; 24 hours) arrests MGC803 cells cycle in G2/M phase[1].
VII-31 (50-150 nM; 48 hours) induces apoptosis via intrinsic and extrinsic pathways[1].
VII-31 (50-150 nM; 24 hours) activates NEDDylation in MGC803 cells[1].
VII-31 (50-150 nM; 48 hours) up-regulates pro-apoptotic proteins FADD, Fasl, PIDD, Bax, Bad; while down-regulates anti-apoptotic proteins Bcl-xL, Bcl-2, XIAP, c-IAP1[1].

Cell Viability Assay[1]

Cell Line: Gastric cancer MGC803 cells
Concentration: 100, 200 nM
Incubation Time: 48 hours
Result: Inhibited the cell viability in dose-depend manner.

Cell Cycle Analysis[1]

Cell Line: MGC803 cells
Concentration: 50, 100, 150 nM
Incubation Time: 24 hours
Result: Arrested cells in G2/M phase, and a clear sub-G1 peak was observed in the high dose group.

Apoptosis Analysis[1]

Cell Line: MGC803 cells
Concentration: 50, 75, 100, and 150 nM
Incubation Time: 48 hours
Result: High dose (150 nM) treatment significantly elevated the early and late apoptosis rate to 92.8% from 4.8%.

Western Blot Analysis[1]

Cell Line: MGC803 cells
Concentration: 50, 100, 150 nM
Incubation Time: 24 hours
Result: Resulted in NEDDylation activation of MGC803 cells, the NEDDylation of 3 important proteins NAE1, Ubc12 and CUL1 has been activated.
In Vivo

VII-31 inhibits the tumor progression in vivo, while showing no obvious toxicity to mice[1].

Animal Model: Mice bearing MGC803 xenograft tumors[1]
Dosage: 50, 100, 150 mg/kg
Administration: Subcutaneous injection; daily for 28 days
Result: The mice had a much smaller tumor compared with vehicle control. The tumor volumes of middle/high dose treated mice at certain time points were evidently decreased comparing with untreated group.
Molecular Weight

427.51

Formula

C₂₃H₂₅NO₅S

CAS No.

2305757-96-2

SMILES

O=C(CC1=CC=CS1)N(CC2=CC=C(OC)C=C2)C3=CC(OC)=C(OC)C(OC)=C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

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Keywords:

VII-31VII31VII 31E1/E2/E3 EnzymeApoptosisE1 activating enzymeE2 conjugating enzymeE3 ligating enzymeUbiquitin activating enzymeUbiquitin conjugating enzymeUbiquitin ligaseNAE1Ubc12Cullin1NEDDylationIAPMGC803MCF-7PC-3Inhibitorinhibitorinhibit

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VII-31
Cat. No.:
HY-133558
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