2. Autophagy
  3. Autophagy
  4. Wogonoside

Wogonoside, a flavonoid glycoside isolated from Huangqin, possesses anti-inflammatory effects. Wogonoside induces autophagy in breast cancer cells by regulating MAPK-mTOR pathway.

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Wogonoside Chemical Structure

Wogonoside Chemical Structure

CAS No. : 51059-44-0

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5 mg USD 55 In-stock
10 mg USD 88 In-stock
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Based on 1 publication(s) in Google Scholar

Wogonoside Related Antibodies

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Description

Wogonoside, a flavonoid glycoside isolated from Huangqin, possesses anti-inflammatory effects. Wogonoside induces autophagy in breast cancer cells by regulating MAPK-mTOR pathway[1][2].

In Vitro

Wogonoside (0-50 μM, 24 h) inhibits the production of inflammatory mediators (NO, PGE2), and pro-inflammatory cytokines (TNF-α and IL-6) in LPS-induced RAW264.7 cells[1].
Wogonoside (0-400 μM, 24 h) inhibits growth of MDA-MB-231 and MCF-7[2].
Wogonoside (100 μM, 9-24 h) induces autophagy in MDA-MB-231 cells[2].
Wogonoside (50-150 μM, 48-96 h) inhibits proliferation, induces G1 phase arrest, and differentiation in U937 and HL-60 cells[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Wogonoside Related Antibodies

Western Blot Analysis[2]

Cell Line: MDA-MB-231 cells
Concentration: 100 μM
Incubation Time: 9-24 h
Result: Increased the expression of LC3-II and Beclin-1 expression.
Inhibited the phosphorylation levels of mTOR at Ser2448 and p70S6K at Ser-389.
In Vivo

Wogonoside (80 mg/kg, oral gavage, once every other day) inhibits tumor growth and metastasis in MDA-MB-231 orthotopic model[3].
Wogonoside (40 or 80 mg/kg, i.p., every three days) inhibits tumor growth and reduces Bcl-2/Bax ratio in A549 cell xenograft mice[4].
Wogonoside (80 mg/kg, i.P., every other day for 14 days) also inhibits tumor growth in U937 xenograft model[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: MDA-MB-231 orthotopic model[3]
Dosage: 80 mg/kg
Administration: oral gavage, once every other day
Result: Inhibited tumor growth (46%) and metastasis in brain, lung, liver and bone.
Increased the expression of E-cadherin and reduced the expression of MMP-9, vimentin and Twist1 at ​orthotopic site.
Molecular Weight

460.39

Formula

C22H20O11
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(C=C(C1=CC=CC=C1)O2)C3=C2C(OC)=C(O[C@@H]4O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]4O)C=C3O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (271.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1721 mL 10.8604 mL 21.7207 mL
5 mM 0.4344 mL 2.1721 mL 4.3441 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (4.52 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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(per animal)

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Dosing volume
(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.86%

COA (252 KB) LCMS (97 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.1721 mL 10.8604 mL 21.7207 mL 54.3018 mL
5 mM 0.4344 mL 2.1721 mL 4.3441 mL 10.8604 mL
10 mM 0.2172 mL 1.0860 mL 2.1721 mL 5.4302 mL
15 mM 0.1448 mL 0.7240 mL 1.4480 mL 3.6201 mL
20 mM 0.1086 mL 0.5430 mL 1.0860 mL 2.7151 mL
25 mM 0.0869 mL 0.4344 mL 0.8688 mL 2.1721 mL
30 mM 0.0724 mL 0.3620 mL 0.7240 mL 1.8101 mL
40 mM 0.0543 mL 0.2715 mL 0.5430 mL 1.3575 mL
50 mM 0.0434 mL 0.2172 mL 0.4344 mL 1.0860 mL
60 mM 0.0362 mL 0.1810 mL 0.3620 mL 0.9050 mL
80 mM 0.0272 mL 0.1358 mL 0.2715 mL 0.6788 mL
100 mM 0.0217 mL 0.1086 mL 0.2172 mL 0.5430 mL
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Wogonoside Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Wogonoside51059-44-0AutophagyInhibitorinhibitorinhibit

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