Cat. No.: HY-75625

2-Hydroxy-4-methoxybenzoic acid

(Synonyms: 4-Methoxysalicylic Acid)

Customer Review

Based on 1 Customer Validation

2-Hydroxy-4-methoxybenzoic acid is an orally active inhibitor of MCT-1 and MCT-4, as well as a plant biomarker. 2-Hydroxy-4-methoxybenzoic acid can be isolated from roots. 2-Hydroxy-4-methoxybenzoic acid induces Apoptosis and loss of mitochondrial membrane potential. 2-Hydroxy-4-methoxybenzoic acid exhibits anticancer activity against breast cancer. 2-Hydroxy-4-methoxybenzoic acid normalizes lactic acid levels. 2-Hydroxy-4-methoxybenzoic acid neutralizes viper venom and attenuates its lethal, hemorrhagic, coagulant and anticoagulant activities in male albino mice. 2-Hydroxy-4-methoxybenzoic acid possesses antihyperlipidemic, antidiabetic and hepatoprotective activities.

For research use only. We do not sell to patients.

Purity: 99.64%
CAS No.: 2237-36-7
Formula: C₈H₈O₄
Molecular Weight:168.15

Storage:

4°C, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

2-Hydroxy-4-methoxybenzoic acid Chemical Structure

CAS No.: 2237-36-7

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

MCT1

MCT4

All Monocarboxylate Transporter Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
RAW264.7	CC₅₀	>40 μM Compound: 2	Cytotoxicity against mouse RAW264.7 cells assessed as reduction in survival rate after 24 hrs by MTT assay Cytotoxicity against mouse RAW264.7 cells assessed as reduction in survival rate after 24 hrs by MTT assay	[PMID: 25200306]

In Vitro

2-Hydroxy-4-methoxybenzoic acid (1-25 μM; 24 h) potently inhibits the viability of MCF-7 breast cancer cells, with an IC₅₀ of 4.8 μM after 24 h of treatment^[2].
2-Hydroxy-4-methoxybenzoic acid (IC₂₅, IC₅₀; 24 h) induces nuclear apoptotic changes in breast cancer MCF-7 cells, with 78% and 87.33% of apoptotic nuclei observed after treatment at IC₂₅ and IC₅₀ concentrations, respectively^[2].
2-Hydroxy-4-methoxybenzoic acid (IC₂₅, IC₅₀; 24 h) induces dose-dependent morphological changes of early and late apoptosis, including membrane blebbing and apoptotic body formation, in MCF-7 breast cancer cells following 24 h of treatment at IC₂₅ and IC₅₀ concentrations^[2].
2-Hydroxy-4-methoxybenzoic acid (IC₂₅, IC₅₀; 24 h) induces loss of mitochondrial membrane potential in breast cancer MCF-7 cells following 24 h of treatment at IC₂₅ and IC₅₀ concentrations^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[2]

Cell Line:

MCF-7 human mammary gland carcinoma cells
Concentration:

1-25 μM
Incubation Time:

24 h
Result:

Reduced MCF-7 cell viability in a concentration-dependent manner, with an IC₅₀ value of 4.8 μM.

Apoptosis Analysis^[2]

Cell Line:

MCF-7 human mammary gland carcinoma cells
Concentration:

IC₂₅, IC₅₀
Incubation Time:

24 h
Result:

Induced bright nuclear condensation and fragmentation compared to untreated cells with uniform round nuclei and faint blue fluorescence.
Reached 78% apoptotic nuclei in the IC₂₅ HMBA group and 87.33% apoptotic nuclei in the IC₅₀ HMBA group, compared to 42% in untreated control cells.\nInduced yellow (early apoptosis) and orange/red (late apoptosis) staining, along with membrane blebbing and apoptotic body formation in a dose-dependent manner compared to untreated cells with uniformly green-stained viable cells and normal large nuclei.\nCaused decreased red fluorescence and increased green fluorescence, indicating loss of MMP, with a reduced red-to-green fluorescence ratio compared to untreated controls with strong red fluorescence indicating high MMP.

In Vivo

2-Hydroxy-4-methoxybenzoic acid (200-400 μg/kg; i.p.) exerts dose-dependent anticancer effects in DMBA-induced breast cancer models of female albino Wistar rats, including reducing tumor proliferation markers, normalizing lactate and antioxidant levels, and regulating the expression of apoptosis- and lactate transporter-related proteins^[2].
2-Hydroxy-4-methoxybenzoic acid (50 mg/20 g mouse) enhances the positive effects of adjuvants and protects mice against venom-induced death and hemorrhage^[1].
2-Hydroxy-4-methoxybenzoic acid (200 μg/kg, p.o.; once daily for 30 days) reduces ethanol-induced hyperlipidemia in male Wistar rats^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Wistar (female, 100-120 g, DMBA-induced mammary gland carcinoma)^[2]
Dosage:

200 μg/kg; 400 μg/kg
Administration:

i.p.
Result:

Reduced terminal end buds count from 95.25 to 73.5 (200 μg/kg) and to 57.25 (400 μg/kg).
Reduced alveolar buds count from 118.75 to 70.25, lobules count from 206 to 154.4, and differentiation score from 420 to 282 (400 μg/kg).
Reduced TBARs levels from 233.97 to 185.90, protein carbonyl levels from 90.98 to 70.45, and increased superoxide dismutase levels from 0.06 to 0.10 (200 μg/kg).
Reduced TBARs levels from 233.97 to 134.62, protein carbonyl levels from 90.98 to 64.55, increased superoxide dismutase levels from 0.06 to 0.11, and increased catalase levels from 5.75 to 7.22 (400 μg/kg).
Normalized elevated serum lactate levels induced by DMBA.
Reduced DMBA-induced overexpression of MCT-1, MCT-4, GLUT-1, HIF-1α, and VEGF; downregulated anti-apoptotic Bcl-2 and Apaf-1, and upregulated pro-apoptotic BAX and Caspase-3.
Improved mammary gland architecture, restoring ordered distribution of luminal epithelial cells, myoepithelial cells, and adipocytes toward normal.

Chemical Information

CAS No. 2237-36-7
Appearance Solid
Molecular Weight 168.15
Formula C₈H₈O₄
Color White to off-white
SMILES

O=C(O)C1=C(O)C=C(OC)C=C1
Synonyms

4-Methoxysalicylic Acid
Structure Classification
- Phenols
- Monophenols
- Ketones, Aldehydes, Acids
Initial Source
- Plants
- other families
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (297.35 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

5.9471 mL

29.7354 mL

59.4707 mL

5 mM

1.1894 mL

5.9471 mL

11.8941 mL

10 mM

0.5947 mL

2.9735 mL

5.9471 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (14.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (14.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (14.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (301 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Nandy S, et al. Indian Sarsaparilla (Hemidesmus indicus): Recent progress in research on ethnobotany, phytochemistry and pharmacology. J Ethnopharmacol. 2020 May 23;254:112609. [Content Brief]

[2]. Yadav S, et al. Anticancer efficacy of 2-Hydroxy-4-methoxybenzoic acid through dual regulation of lactate transport via inhibition of MCT-1 and MCT-4[J]. Advances in Cancer Biology-Metastasis, 2025: 100163.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	5.9471 mL	29.7354 mL	59.4707 mL	148.6768 mL
	5 mM	1.1894 mL	5.9471 mL	11.8941 mL	29.7354 mL
	10 mM	0.5947 mL	2.9735 mL	5.9471 mL	14.8677 mL
	15 mM	0.3965 mL	1.9824 mL	3.9647 mL	9.9118 mL
	20 mM	0.2974 mL	1.4868 mL	2.9735 mL	7.4338 mL
	25 mM	0.2379 mL	1.1894 mL	2.3788 mL	5.9471 mL
	30 mM	0.1982 mL	0.9912 mL	1.9824 mL	4.9559 mL
	40 mM	0.1487 mL	0.7434 mL	1.4868 mL	3.7169 mL
	50 mM	0.1189 mL	0.5947 mL	1.1894 mL	2.9735 mL
	60 mM	0.0991 mL	0.4956 mL	0.9912 mL	2.4779 mL
	80 mM	0.0743 mL	0.3717 mL	0.7434 mL	1.8585 mL
	100 mM	0.0595 mL	0.2974 mL	0.5947 mL	1.4868 mL

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2-Hydroxy-4-methoxybenzoic acid

Apoptosis Compound Library

Membrane Transporter/Ion Channel Compound Library

Metabolism/Protease Compound Library

Stem Cell Signaling Compound Library

Natural Product Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Reprogramming Compound Library

Anti-diabetic Compound Library

Anti-Cardiovascular Disease Compound Library

Medicine Food Homology Compound Library

Phenols Library

Orally Active Compound Library

Glutamine Metabolism Compound Library

Traditional Chinese Medicine Active Compound Library

Anti-Breast Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Anti-Parkinson's Disease Compound Library

Neurodegenerative Disease-related Compound Library

Mitochondria-Targeted Compound Library

Glucose Metabolism Compound Library

Food-Sourced Compound Library

Anti-Cancer Natural Product Library

Plant-Sourced Natural Product Library

Cuproptosis Compound Library

Cancer Stem Cells Compound Library

Mitochondrial Protection Compound Library

Metabolic Enzyme Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Cell Death Library

Multi-Target Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

RO5 Drug-like Natural Product Library

Lactic Acid Metabolic Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Mass Spectrometry Natural Product Library

All Monocarboxylate Transporter Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

2-Hydroxy-4-methoxybenzoic acid Related Classifications

Cat. No.: HY-75625