2. Apoptosis
  3. PKD
  4. 3-IN-PP1

3-IN-PP1 is a protein kinase D (PKD) inhibitor. 3-IN-PP1 has potent pan-PKD inhibitory activity for PKD1, PKD2 and PKD3 with IC50 values of 108, 94 and 108 nM, respectively. 3-IN-PP1 also is a broad spectrum anticancer agent and has inhibition of several tumor cells growth. 3-IN-PP1 can be used for the research of cancer.

For research use only. We do not sell to patients.

3-IN-PP1 Chemical Structure

3-IN-PP1 Chemical Structure

CAS No. : 2227110-54-3

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 242 In-stock
Solution
10 mM * 1 mL in DMSO USD 242 In-stock
Solid
5 mg USD 220 In-stock
10 mg USD 360 In-stock
25 mg USD 720 In-stock
50 mg USD 1150 In-stock
100 mg USD 1850 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

3-IN-PP1 Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

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Description

3-IN-PP1 is a protein kinase D (PKD) inhibitor. 3-IN-PP1 has potent pan-PKD inhibitory activity for PKD1, PKD2 and PKD3 with IC50 values of 108, 94 and 108 nM, respectively. 3-IN-PP1 also is a broad spectrum anticancer agent and has inhibition of several tumor cells growth. 3-IN-PP1 can be used for the research of cancer[1].

IC50 & Target[1]

PKD1

108 nM (IC50)

Cellular PKD2

94 nM (IC50)

PKD3

108 nM (IC50)

In Vitro

3-IN-PP1 has potent pan-PKD inhibitory activity for PKD1, PKD2 and PKD3 with IC50 values of 108, 94 and 108 nM, respectively[1].
3-IN-PP1 (5 μM, 0-114 h) displays potent anti-proliferative activity against PANC-1 cells[1].
3-IN-PP1 (20 μM, 1 h) potently blocks PKD-dependent cortactin phosphorylation in PANC-1 cells[1].
3-IN-PP1 has inhibition of different tumor cell growth with IC50 values range from 1.6 to 39.2μM[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

3-IN-PP1 Related Antibodies

Cell Proliferation Assay[1]

Cell Line: PANC-1 cell
Concentration: 5 μM
Incubation Time: 0-114 h
Result: Significantly inhibited PANC-1 cell proliferation after incubation for 96 and 144 hours.

Western Blot Analysis[1]

Cell Line: PANC-1 cells
Concentration: 20 μM
Incubation Time: 1 h
Result: Reduced cortactin phosphorylation in PANC-1 cells.
Molecular Weight

306.37

Formula

C17H18N6
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

NC1=C2C(N(C(C)(C)C)N=C2C3=CNC4=C3C=CC=C4)=NC=N1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (326.40 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2640 mL 16.3201 mL 32.6403 mL
5 mM 0.6528 mL 3.2640 mL 6.5281 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (8.16 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (8.16 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.2640 mL 16.3201 mL 32.6403 mL 81.6007 mL
5 mM 0.6528 mL 3.2640 mL 6.5281 mL 16.3201 mL
10 mM 0.3264 mL 1.6320 mL 3.2640 mL 8.1601 mL
15 mM 0.2176 mL 1.0880 mL 2.1760 mL 5.4400 mL
20 mM 0.1632 mL 0.8160 mL 1.6320 mL 4.0800 mL
25 mM 0.1306 mL 0.6528 mL 1.3056 mL 3.2640 mL
30 mM 0.1088 mL 0.5440 mL 1.0880 mL 2.7200 mL
40 mM 0.0816 mL 0.4080 mL 0.8160 mL 2.0400 mL
50 mM 0.0653 mL 0.3264 mL 0.6528 mL 1.6320 mL
60 mM 0.0544 mL 0.2720 mL 0.5440 mL 1.3600 mL
80 mM 0.0408 mL 0.2040 mL 0.4080 mL 1.0200 mL
100 mM 0.0326 mL 0.1632 mL 0.3264 mL 0.8160 mL
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3-IN-PP1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

3-IN-PP12227110-54-3PKDProtein kinase Dprotein kinase D (PKD)antitumorPKD1PKD2PKD3broad spectrum anticancercancerInhibitorinhibitorinhibit

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