1. Metabolic Enzyme/Protease
  2. Proteasome
  3. 4'-Hydroxychalcone

4'-Hydroxychalcone 

Cat. No.: HY-N7056 Purity: >98.0%
Handling Instructions

4'-Hydroxychalcone is a chalcone isolated from licorice root, with hepatoprotective activity. 4'-Hydroxychalcone inhibits TNFα-induced NF-κB activation via proteasome inhibition. 4'-Hydroxychalcone induces a rapid potassium release from mitochondrial vesicles and causes deterioration of respiratory control and oxidative phosphorylation of isolated rat liver mitochondria.

For research use only. We do not sell to patients.

4'-Hydroxychalcone Chemical Structure

4'-Hydroxychalcone Chemical Structure

CAS No. : 2657-25-2

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10 mM * 1  mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
100 mg USD 60 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

4'-Hydroxychalcone is a chalcone isolated from licorice root, with hepatoprotective activity. 4'-Hydroxychalcone inhibits TNFα-induced NF-κB activation via proteasome inhibition. 4'-Hydroxychalcone induces a rapid potassium release from mitochondrial vesicles and causes deterioration of respiratory control and oxidative phosphorylation of isolated rat liver mitochondria[1][2][3].

In Vitro

4'-Hydroxychalcone (20-40 μM ; 2 hours) inhibits TNFα-induced (20 ng/mL; 6 hours) NF-kB pathway activation in a dose-dependent manner[3].
4'-Hydroxychalcone (0.1-25 μM ; 8 hours) inhibits proteasome activity in a dose-dependent manner but has no effect on IKK activity[3].
4'-Hydroxychalcone inhibits TNFα-dependent degradation of IkBα and subsequently prevents p50/p65 nuclear translocation leading to 4'-Hydroxychalcone-inhibited expression of NF-kB target genes[3].
4'-Hydroxychalcone affects cancer cell viability but has no significant effect on non-transformed cell viability[3].

Cell Viability Assay[3]

Cell Line: K562 cells, Jurkat cells, U937 cells, PBMCs
Concentration: 5 μM, 10 μM, 20 μM, 24 μM, 28 μM, 32 μM, 40 μM, 60 μM
Incubation Time: 24 hours
Result: Affected cancer cell viability but has no significant effect on non-transformed cell viability.

Western Blot Analysis[3]

Cell Line: Jurkat cells
Concentration: 60 μM (followed by TNFα 20 ng/mL )
Incubation Time: 2 hours
Result: Inhibited TNFα -dependent degradation of IkBα and prevents p50/p65 nuclear translocation.
In Vivo

4'-Hydroxychalcone has hepatoprotective activity against Acetaminophen induced hepatotoxicity in mice[2].

Animal Model: Male albino mice(5-30 g)[2]
Dosage: 25 mg/kg, 50 mg/kg, 100 mg/kg
Administration: Oral administration, 12h intervals, 4 doses
Result: Significantly reduced the mortality rate induced by Acetaminophen (1 g/kg).
Molecular Weight

224.25

Formula

C₁₅H₁₂O₂

CAS No.

2657-25-2

SMILES

O=C(C1=CC=C(O)C=C1)/C=C/C2=CC=CC=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (1114.83 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.4593 mL 22.2965 mL 44.5931 mL
5 mM 0.8919 mL 4.4593 mL 8.9186 mL
10 mM 0.4459 mL 2.2297 mL 4.4593 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (9.28 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (9.28 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (9.28 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Keywords:

4'-HydroxychalconeProteasomechalconehepatoprotectiveproteasomepotassiummitochondrialvesiclesoxidativephosphorylationInhibitorinhibitorinhibit

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Product name:
4'-Hydroxychalcone
Cat. No.:
HY-N7056
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