8-Hydroxyefavirenz
Based on 1 Customer Validation
8-Hydroxyefavirenz (8-OH-EFV) is a primary metabolite of (HY-10572). 8-Hydroxyefavirenz induces apoptosis via a JNK- and BimEL-dependent mechanism in primary human hepatocytes. 8-Hydroxyefavirenz can be used in research of cancer. 8-Hydroxyefavirenz is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
For research use only. We do not sell to patients.
- Purity: 97.01%
- CAS No.: 205754-33-2
- Formula: C14H9ClF3NO3
- Molecular Weight:331.67
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
8-Hydroxyefavirenz (8-OH-EFV; 1-10 μM; 3-24 h; primary human hepatocytes) increases cell death in a time- and concentration-dependent manner and induces caspase-3 activity beginning at 6 h[1].
8-Hydroxyefavirenz (1-10 μM; 6-24 h) stimulates mitochondria ROS production in primary human hepatocytes[1].
8-Hydroxyefavirenz (10 μM; 3-24 h) activates JNK and increases the ratio of phosphorylated JNK to total JNK by 4.2-fold. 8-Hydroxyefavirenz increases the mRNA and protein expression of BimEL[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Primary human hepatocytes
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Concentration:1 and 10 μM
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Incubation Time:3, 6, 12 and 24 hours
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Result:Increased cell death in a time- and concentration-dependent manner and increased cell death by 3.4-fold at 6 h.
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Cell Line:Primary human hepatocytes
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Concentration:1 and 10 μM
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Incubation Time:3, 6, 12 and 24 hours
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Result:Increased the expression of cleaved caspase-3 in a time- and concentration-dependent manner.
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Cell Line:Primary human hepatocytes
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Concentration:1 and 10 μM
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Incubation Time:3, 6, 12 and 24 hours
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Result:Increased the phosphorylation of JNK and increased the mRNA and protein expression of BimEL.
Chemical Information
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CAS No. 205754-33-2
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Appearance Solid
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Molecular Weight 331.67
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Formula C14H9ClF3NO3
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Color White to off-white
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SMILES
O=C1NC2=C(O)C=C(Cl)C=C2[C@](C(F)(F)F)(C#CC3CC3)O1
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Synonyms
8-OH-EFV
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 250 mg/mL (753.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (279 KB)
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SDS (761 KB)
- English - EN (761 KB)
- Français - FR (761 KB)
- Deutsch - DE (761 KB)
- Norwegian - NO (761 KB)
- Español - ES (761 KB)
- Swedish - SV (761 KB)
- Italian - IT (761 KB)
- Portuguese - PT (761 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.0150 mL | 15.0752 mL | 30.1504 mL | 75.3761 mL |
| 5 mM | 0.6030 mL | 3.0150 mL | 6.0301 mL | 15.0752 mL | |
| 10 mM | 0.3015 mL | 1.5075 mL | 3.0150 mL | 7.5376 mL | |
| 15 mM | 0.2010 mL | 1.0050 mL | 2.0100 mL | 5.0251 mL | |
| 20 mM | 0.1508 mL | 0.7538 mL | 1.5075 mL | 3.7688 mL | |
| 25 mM | 0.1206 mL | 0.6030 mL | 1.2060 mL | 3.0150 mL | |
| 30 mM | 0.1005 mL | 0.5025 mL | 1.0050 mL | 2.5125 mL | |
| 40 mM | 0.0754 mL | 0.3769 mL | 0.7538 mL | 1.8844 mL | |
| 50 mM | 0.0603 mL | 0.3015 mL | 0.6030 mL | 1.5075 mL | |
| 60 mM | 0.0503 mL | 0.2513 mL | 0.5025 mL | 1.2563 mL | |
| 80 mM | 0.0377 mL | 0.1884 mL | 0.3769 mL | 0.9422 mL | |
| 100 mM | 0.0302 mL | 0.1508 mL | 0.3015 mL | 0.7538 mL |
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